Copper(II) and iron(III) ions inhibit respiration and increase free radical-mediated phospholipid peroxidation in rat liver mitochondria: Effect of antioxidants

2017 ◽  
Vol 172 ◽  
pp. 94-99 ◽  
Author(s):  
Christian Saporito-Magriñá ◽  
Rosario Musacco-Sebio ◽  
Juan M. Acosta ◽  
Sofía Bajicoff ◽  
Paola Paredes-Fleitas ◽  
...  
Keyword(s):  
Free Radical ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Phospholipid Peroxidation

scholarly journals THE RATIO OF UBIQUINON REDOX FORMS IN THE RAT LIVER MITOCHONDRIA UNDER CONDITIONS OF DIFFERENT NUTRIENT SUPPLY

2020 ◽  
Vol 66 (6) ◽  
pp. 82-87
Author(s):  
O.M. Voloshchuk ◽  
◽  
G. P. Kopylchuk ◽  
М.S. Ursatyу ◽  
◽  
...  
Keyword(s):  
Free Radical ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Oxidative Modification ◽  
Mitochondrial Proteins ◽  
Rat Liver Mitochondria ◽  
Carbonyl Derivatives ◽  
High Sucrose Diet ◽  
Redox Forms ◽  
High Sucrose

The relationship between the quantitative ratio of redox forms of ubiquinone and the degree of free radical damage to mitochondrial proteins in rat liver against the background of nutritional imbalance was investigated. The animals were divided into the following experimental groups: I – animals receiving full-value semi-synthetic ration (control group); II – animals receiving high-sucrose diet; III – animals receiving low-protein high-sucrose diet. The content of total and oxidized ubiquinone was determined spectrophotometrically at 275 nm, the content of reduced ubiquinone was determined by the difference between the content of total and oxidized ubiquinone. The intensity of the oxidative modification of proteins was assessed by the accumulation of carbonyl derivatives in the reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH), the content of free SH-groups was assessed by using the Elman reagent. It was found that the most pronounced decrease in the content of total ubiquinone (almost twice) and the redistribution of its redox forms (reduction of the content of reduced ubiquinone by 7.2 times against the background of an increase in the level of oxidized ubiquinone by 2 times) in rat liver mitochondria is observed in animals that received a diet high in sucrose against the background of alimentary protein deprivation. In addition, the animals of this group showed the most pronounced free radical oxidation of mitochondrial proteins, as evidenced by a 3.5-fold increase in the content of carbonyl derivatives and a 2.6-fold decrease in the content of free protein SH- groups. It was shown that nutritional protein deficiency is a critical factor affecting the intensity of free radical processes in mitochondria. The established changes in the ratio of the redox forms of ubiquinone and the degree of oxidative modification of mitochondrial proteins in rat liver could be considered as prerequisites for deepening the energy imbalance and violation of the functional activity of mitochondria under conditions of nutritional imbalance.

scholarly journals Free-radical metabolism of carbon tetrachloride in rat liver mitochondria. A study of the mechanism of activation

1987 ◽  
Vol 246 (2) ◽  
pp. 313-317 ◽  
Author(s):  
A Tomasi ◽  
E Albano ◽  
S Banni ◽  
B Botti ◽  
F Corongiu ◽  
...  
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Radical Species ◽  
Hypoxic Conditions ◽  
Free Radical Species ◽  
Transport Chain

Alterations in liver mitochondria as consequence of rat poisoning with carbon tetrachloride (CCl4) have been reported over many years, but the mechanisms responsible for causing such damage are still largely unknown. Isolated rat liver mitochondria incubated under hypoxic conditions with succinate and ADP were found able to activate CCl4 to a free-radical species identified as trichloromethyl free radical (CCl3) by e.s.r. spectroscopy coupled with the spin-trapping technique. The incubation of mitochondria in air decreased free-radical production, indicating that a reductive reaction was involved in the activation of CCl4. However, in contrast with liver microsomes (microsomal fractions), mitochondria did not require the presence of NADPH, and the process was not significantly influenced by inhibitors of cytochrome P-450. The addition of inhibitors of the respiratory chain such as antimycin A and KCN decreased free-radical formation by only 30%, whereas rotenone displayed a greater effect (approx. 84% inhibition), but only when preincubated for 15 min with mitochondria not supplemented with succinate. These findings suggest that the mitochondrial electron-transport chain is responsible for the activation of CCl4. A conjugated-diene band was observed in the lipids extracted from mitochondria incubated with CCl4 under anaerobic conditions, indicating that stimulation of lipid peroxidation was occurring as a result of the formation of free-radical species.

Free radical scavenging action of the natural polyamine spermine in rat liver mitochondria

2006 ◽  
Vol 41 (8) ◽  
pp. 1272-1281 ◽  
Author(s):  
Irina G. Sava ◽  
Valentina Battaglia ◽  
Carlo A. Rossi ◽  
Mauro Salvi ◽  
Antonio Toninello
Keyword(s):  
Free Radical ◽  
Rat Liver ◽  
Radical Scavenging ◽  
Liver Mitochondria ◽  
Free Radical Scavenging ◽  
Rat Liver Mitochondria

Electromagnetic pulse reduces free radical generation in rat liver mitochondria invitro

2013 ◽  
Vol 47 (4) ◽  
pp. 276-282 ◽  
Author(s):  
C. Wang ◽  
H. Zhou ◽  
R. Peng ◽  
L. Wang ◽  
Z. Su ◽  
...  
Keyword(s):  
Free Radical ◽  
Rat Liver ◽  
Electromagnetic Pulse ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Free Radical Generation ◽  
Radical Generation

Effects of 5-5'-Diphenyl-2-thiohydantoin (DPTH) and Thyroxine on the Ultrastructure and Chemical Composition of Rat Liver

1971 ◽  
Vol 29 ◽  
pp. 570-571
Author(s):  
E. A. Elfont ◽  
R. B. Tobin ◽  
D. G. Colton ◽  
M. A. Mehlman
Keyword(s):  
Chemical Composition ◽  
Rat Liver ◽  
Future Study ◽  
Mode Of Action ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Effective Inhibitor ◽  
Biochemical Effects ◽  
Glycerophosphate Dehydrogenase ◽  
Stimulation Of

Summary5,-5'-diphenyl-2-thiohydantoin (DPTH) is an effective inhibitor of thyroxine (T4) stimulation of α-glycerophosphate dehydrogenase in rat liver mitochondria. Because this finding indicated a possible tool for future study of the mode of action of thyroxine, the ultrastructural and biochemical effects of DPTH and/or thyroxine on rat liver mere investigated.Rats were fed either standard or DPTH (0.06%) diet for 30 days before T4 (250 ug/kg/day) was injected. Injection of T4 occurred daily for 10 days prior to sacrifice. After removal of the liver and kidneys, part of the tissue was frozen at -50°C for later biocheailcal analyses, while the rest was prefixed in buffered 3.5X glutaraldehyde (390 mOs) and post-fixed in buffered 1Z OsO4 (376 mOs). Tissues were embedded in Araldlte 502 and the sections examined in a Zeiss EM 9S.Hepatocytes from hyperthyroid rats (Fig. 2) demonstrated enlarged and more numerous mitochondria than those of controls (Fig. 1). Glycogen was almost totally absent from the cytoplasm of the T4-treated rats.

Energy and Antioxidant Status of Rat Liver Mitochondria during Hypoxia- Reoxygenation of Different Duration

2016 ◽  
Vol 7 (4) ◽  
pp. 349-361
Author(s):  
Olga A. Gonchar ◽  
Valentina I. Nosar ◽  
Larisa. V. Bratus ◽  
I. N. Tymchenko ◽  
N. N. Steshenko ◽  
...  
Keyword(s):  
Rat Liver ◽  
Antioxidant Status ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Hypoxia Reoxygenation
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