scholarly journals Protein dynamics of [Cu-Zn] superoxide dismutase (SOD1): How protein motions at the global and local levels impact the reactivity of SOD1

2020 ◽  
Vol 210 ◽  
pp. 111161
Author(s):  
Eamonn F. Healy ◽  
Rafael Flores ◽  
Vincent M. Lynch ◽  
Santiago Toledo
2019 ◽  
Vol 3 (1) ◽  
pp. 53-81
Author(s):  
Julia McClure ◽  
Amitava Chowdhury ◽  
Sarah Easterby-Smith ◽  
Norberto Ferreras ◽  
Omar Gueye ◽  
...  

The following is an edited transcript of a roundtable that took place at the University of Glasgow in September 2018. The roundtable was organized by Dr. Julia McClure in conjunction with the Poverty Research Network’s conference - Beyond Development: The Local Visions of Global Poverty. That conference brought into focus the ways in which the global and local levels meet at the site of poverty and highlighted the different conceptions on the global are generated from the perspective of poverty. The roundtable brought together leading scholars from Europe, Africa, Asia and North and South America to take stock of global history as a field, to consider the role of existing centres of knowledge production, and to assess new directions for the field.


Author(s):  
Petros Ieromonachou ◽  
Nola Stair

Technology is always advancing; furthermore, the economy and demographics, both at global and local levels dictate changes that businesses need to consider for future planning. Like all businesses, most academic institutions need to follow trends and offer new and up-to-date learning initiatives. This chapter discusses the assessment and evaluation of Web-based postgraduate and undergraduate programmes, at the Business School of a London-based university. The study reviews relevant literature, investigates student and staff views on the use of technology in learning, and reflects on the planning and management of two Supported Open Learning (SOL) programmes. It also offers suggestions for future programmes and courses designed for use with e-learning technologies.


2020 ◽  
Vol 7 ◽  
Author(s):  
Lorea Alejaldre ◽  
Claudèle Lemay-St-Denis ◽  
Carles Perez Lopez ◽  
Ferran Sancho Jodar ◽  
Victor Guallar ◽  
...  

The evolution of new protein functions is dependent upon inherent biophysical features of proteins. Whereas, it has been shown that changes in protein dynamics can occur in the course of directed molecular evolution trajectories and contribute to new function, it is not known whether varying protein dynamics modify the course of evolution. We investigate this question using three related ß-lactamases displaying dynamics that differ broadly at the slow timescale that corresponds to catalytic turnover yet have similar fast dynamics, thermal stability, catalytic, and substrate recognition profiles. Introduction of substitutions E104K and G238S, that are known to have a synergistic effect on function in the parent ß-lactamase, showed similar increases in catalytic efficiency toward cefotaxime in the related ß-lactamases. Molecular simulations using Protein Energy Landscape Exploration reveal that this results from stabilizing the catalytically-productive conformations, demonstrating the dominance of the synergistic effect of the E014K and G238S substitutions in vitro in contexts that vary in terms of sequence and dynamics. Furthermore, three rounds of directed molecular evolution demonstrated that known cefotaximase-enhancing mutations were accessible regardless of the differences in dynamics. Interestingly, specific sequence differences between the related ß-lactamases were shown to have a higher effect in evolutionary outcomes than did differences in dynamics. Overall, these ß-lactamase models show tolerance to protein dynamics at the timescale of catalytic turnover in the evolution of a new function.


Author(s):  
Sayan Sakhakarmi ◽  
Jee Woong Park

A traditional structural analysis of scaffolding structures requires loading conditions that are only possible during design, but not in operation. Thus, this study proposes a method that can be used during operation to make an automated safety prediction for scaffolds. It implements a divide-and-conquer technique with deep learning. As a test scaffolding, a four-bay, three-story scaffold model was used. Analysis of the model led to 1411 unique safety cases for the model. To apply deep learning, a test simulation generated 1,540,000 datasets for pre-training, and an additional 141,100 datasets for testing purposes. The cases were then sub-divided into 18 categories based on failure modes at both global and local levels, along with a combination of member failures. Accordingly, the divide-and-conquer technique was applied to the 18 categories, each of which were pre-trained by a neural network. For the test datasets, the overall accuracy was 99%. The prediction model showed that 82.78% of the 1411 safety cases showed 100% accuracy for the test datasets, which contributed to the high accuracy. In addition, the higher values of precision, recall, and F1 score for the majority of the safety cases indicate good performance of the model, and a significant improvement compared with past research conducted on simpler cases. Specifically, the method demonstrated improved performance with respect to accuracy and the number of classifications. Thus, the results suggest that the methodology could be reliably applied for the safety assessment of scaffolding systems that are more complex than systems tested in past studies. Furthermore, the implemented methodology can easily be replicated for other classification problems.


2011 ◽  
Vol 125 (1) ◽  
pp. 165-172 ◽  
Author(s):  
Tejpal Gill ◽  
Vivek Dogra ◽  
Sanjay Kumar ◽  
Paramvir Singh Ahuja ◽  
Yelam Sreenivasulu

1994 ◽  
Vol 72 (7) ◽  
pp. 897-914 ◽  
Author(s):  
Isabelle Goulet ◽  
Denis Barabé ◽  
Luc Brouillet

The inflorescence structure of Begoniaceae was analyzed taking into account their degree of symmetry. Qualitative and quantitative analyses of 71 species led to the recognition of nine architectural models. When the whole plant is considered, the inflorescence of Begoniaceae is a thyrse. Comparative analyses of the models allowed to determine at what level symmetry or asymmetry is found. There is a correlation between the total lengths of axes and between flower numbers on the best and least developed sides of an inflorescence. For the whole family, the difference between the two sides of the inflorescence is significant for the former, and not for the latter. Divergence angles on the best developed side do not differ from those on the least developed one for a same ramification, and likewise between successive levels of ramification. Thus, the inflorescence appears asymmetrical if total length of axes is considered. These results tend to support the hypothesis that the Begoniaceae are fundamentally asymmetrical. Inflorescence symmetry is found in some groups by stabilization and compensation of different asymmetric components at the global and local levels. Nevertheless, inflorescence asymmetry does not seem to be related to plant architecture, although it seems to be related to the degree of foliar asymmetry. Key words: Begoniaceae, inflorescence, cyme, inflorescential architecture, structural asymmetry.


1999 ◽  
Vol 566 ◽  
Author(s):  
J. Mendonca ◽  
C. Dang ◽  
S. Selinidis ◽  
M. Angyal ◽  
B. Boeck ◽  
...  

Various options that afford control of the TEOS, W, and Cu/barrier polishes were explored in the building of multilevel dual inlaid structures. Improved tool performance that enables more sophisticated down pressure control with higher resolution backpressure adjustments was employed for the oxide module to control the interlevel capacitances. Planarity at both the global and local levels at the oxide polish affords a good starting point for successive builds without metal pooling. In W CMP, small and controllable oxide erosion and plug recess was obtained with harder polishing pads. In Cu/barrier CMP, the tight overpolish/underpolish margin was maintained by head control and appropriate endpoint algorithms. A six-level build with tight and low sheet resistances and leakages was demonstrated.


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