A whole genome screening and RNA interference identify a juvenile hormone esterase-like gene of the diamondback moth, Plutella xylostella

2015 ◽  
Vol 80 ◽  
pp. 81-87 ◽  
Author(s):  
Xiaojun Gu ◽  
Sunil Kumar ◽  
Eunjin Kim ◽  
Yonggyun Kim
Keyword(s):  
Rna Interference ◽  
Juvenile Hormone ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Whole Genome ◽  
Juvenile Hormone Esterase ◽  
Genome Screening

Effect of Treatment With 3-Octylthio-1,1,1-Trifluoropropan-2-One in the Diamondback Moth (Lepidoptera: Plutellidae) to the Toxicity of Diafenthiuron, Indoxacarb, and Bacillus thuringiensis

2020 ◽  
Vol 113 (3) ◽  
pp. 1419-1425 ◽  
Author(s):  
Jingfei Huang ◽  
Sufen Tian ◽  
Ke Ren ◽  
Yong Chen ◽  
Shuo Lin ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Juvenile Hormone ◽  
Combination Treatment ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Treatment Method ◽  
Juvenile Hormone Esterase ◽  
Trifluoromethyl Ketones ◽  
Insecticide Synergist ◽  
Almost All

Abstract The diamondback moth, Plutella xylostella (L.), is a worldwide insect pest of cruciferous crops. Although insecticides have long been used for its control, diamondback moth rapidly evolves resistance to almost any insecticide. In insects, juvenile hormone (JH) is critically involved in almost all biological processes. The correct activity of JH depends on the precise regulation of its titer, and juvenile hormone esterase (JHE) is the key regulator. Thus, JH and JHE have become important targets for new insecticide development. Trifluoromethyl ketones are specific JHE inhibitors, among which 3-octylthio-1,1,1-trifluoropropan-2-one (OTFP) has the highest activity. The interaction effects between pretreatment with or combination of OTFP and the insecticides diafenthiuron, indoxacarb, and Bacillus thuringiensis (Bt) were investigated in diamondback moth larvae to determine OTFP’s potential as an insecticide synergist. In third-instar larvae, both pretreatment and combination treatment with OTFP decreased or antagonized the toxicities of diafenthiuron, indoxacarb, and Bt at all set concentrations. In fourth-instar larvae, combination treatment with OTFP decreased or antagonized the toxicities of diafenthiuron and indoxacarb at all set concentrations. However, it increased or synergized the toxicity of Bt at lower concentrations despite the limited effect at higher concentrations. Our results indicated that the effect of OTFP on the toxicities of insecticides varied with the type and concentration, larval stage, and treatment method. These findings contribute to the better use of OTFP in diamondback moth control.

scholarly journals Functional Characterization of a Juvenile Hormone Esterase Related Gene in the Moth Sesamia nonagrioides through RNA Interference

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73834 ◽  
Author(s):  
Dimitrios Kontogiannatos ◽  
Luc Swevers ◽  
Katsumi Maenaka ◽  
Enoch Y. Park ◽  
Kostas Iatrou ◽  
...  
Keyword(s):  
Rna Interference ◽  
Juvenile Hormone ◽  
Functional Characterization ◽  
Sesamia Nonagrioides ◽  
Related Gene ◽  
Juvenile Hormone Esterase

scholarly journals Identification of a novel cytochrome P450 gene,CYP321E1from the diamondback moth,Plutella xylostella(L.) and RNA interference to evaluate its role in chlorantraniliprole resistance

2014 ◽  
Vol 104 (6) ◽  
pp. 716-723 ◽  
Author(s):  
Z. Hu ◽  
Q. Lin ◽  
H. Chen ◽  
Z. Li ◽  
F. Yin ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rna Interference ◽  
Plutella Xylostella ◽  
Developmental Stages ◽  
Diamondback Moth ◽  
Fourth Instar ◽  
Cytochrome P450 Monooxygenases ◽  
Functional Link ◽  
Cytochrome P450 Gene ◽  
P450 Gene

AbstractInsect cytochrome P450 monooxygenases (P450s) play an important role in catalysis of many reactions leading to insecticides resistance. Our previous studies on transcriptome analysis of chlorantraniliprole-resistant development in the diamondback moth,Plutella xylostellarevealed that up-regulation of cytochrome P450s are one of the main factors leading to the development of chlorantraniliprole resistance. Here, we report for the first time a novel cytochrome P450 geneCYP321E1, which belongs to the cytochrome P450 gene family CYP321. Real-time quantitative PCR (RT–qPCR) analyses indicated thatCYP321E1was expressed at all developmental stages ofP. xylostellabut was highest in the fourth-instar larvae; furthermore, the relatively high expression was observed in the midgut of the fourth-instar larvae, followed by fat bodies and epidermis. The expression ofCYP321E1inP. xylostellawas differentially affected by three representative insecticides, including alphamethrin, abamectin and chlorantraniliprole. Among them, the exposure to chlorantraniliprole resulted in the largest transcript level of this cytochrome P450 gene. The findings suggested potential involvement ofCYP321E1in chlorantraniliprole resistance ofP. xylostella. To assess the functional link ofCYP321E1to chlorantraniliprole resistance, RNA interference (RNAi)-mediated gene silencing by double stranded RNA (dsRNA) injecting was used. Results revealed that injection delivery of dsRNA can greatly reduce gene expression after 24 h. As a consequence of RNAi, a significant increment in mortality of larvae injectedCYP321E1dsRNA was observed after 24 h of exposure to chlorantraniliprole. These results strongly support our notion that this novel cytochrome P450 gene plays an important role in chlorantraniliprole detoxification in the diamondback moth and is partly responsible for its resistance.

Sign in / Sign up

Export Citation Format

Share Document