First report of the protozoan parasite Perkinsus marinus in South America, infecting mangrove oysters Crassostrea rhizophorae from the Paraíba River (NE, Brazil)

2013 ◽  
Vol 113 (1) ◽  
pp. 96-103 ◽  
Author(s):  
Patricia Mirella da Silva ◽  
Rogério Tubino Vianna ◽  
Cristhiane Guertler ◽  
Liana Pinho Ferreira ◽  
Lucas Nunes Santana ◽  
...  
Keyword(s):  
South America ◽  
Protozoan Parasite ◽  
Perkinsus Marinus ◽  
First Report ◽  
Crassostrea Rhizophorae

Parasites infecting the cultured oyster Crassostrea gasar (Adanson, 1757) in Northeast Brazil

Parasitology ◽  
2015 ◽  
Vol 142 (6) ◽  
pp. 756-766 ◽  
Author(s):  
FERNANDO RAMOS QUEIROGA ◽  
ROGÉRIO TUBINO VIANNA ◽  
CAIRÉ BARRETO VIEIRA ◽  
NATANAEL DANTAS FARIAS ◽  
PATRICIA MIRELLA DA SILVA
Keyword(s):  
Local Population ◽  
Protozoan Parasite ◽  
The Other ◽  
Histopathological Analysis ◽  
Northeast Brazil ◽  
Perkinsus Marinus ◽  
Molecular Analyses ◽  
First Report ◽  
Oyster Species ◽  
Symbiotic Organisms

SUMMARYThe oyster Crassostrea gasar is a species widely used as food and a source of income for the local population of the estuaries of Northeast Brazil. Perkinsus marinus and Perkinsus olseni are deleterious parasites for oyster farming and were recently detected in Brazil. In this study, a histopathologic survey of the oyster C. gasar cultured in the estuary of the River Mamanguape (Paraíba State) was performed. Adult oysters were collected in December 2011 and March, May, August and October 2012 and processed for histology and Perkinsus sp. identification by molecular analyses. Histopathological analysis revealed the presence of parasitic organisms including viral gametocytic hypertrophy, prokaryote-like colonies, protozoans (Perkinsus sp. and Nematopsis sp.) and metazoans (Tylocephalum sp. and cestodes). Other commensal organisms were also detected (the protozoan Ancistrocoma sp. and the turbellarian Urastoma sp.). The protozoan parasite Perkinsus sp. had the highest overall prevalence among the symbiotic organisms studied (48·9%), followed by Nematopsis sp. (36·3%). The other organisms were only sporadically observed. Only the protozoan Perkinsus sp. caused alterations in the oysters’ infected organs. Molecular analyses confirmed the presence of P. marinus, P. olseni and Perkinsus beihaiensis infecting the oyster C. gasar. This is the first report of P. beihaiensis in this oyster species.

Host oyster tissue extracts modulate in vitro protease expression and cellular differentiation in the protozoan parasite, Perkinsus marinus

Parasitology ◽  
2003 ◽  
Vol 126 (4) ◽  
pp. 293-302 ◽  
Author(s):  
E. A. MACINTYRE ◽  
C. G. EARNHART ◽  
S. L. KAATTARI
Keyword(s):  
Serine Proteases ◽  
Protozoan Parasite ◽  
Eastern Oyster ◽  
Defined Medium ◽  
Tissue Homogenate ◽  
Oyster Tissue ◽  
Perkinsus Marinus ◽  
Tissue Extracts

Perkinsus marinus is responsible for a chronic disease (Dermo) of the Eastern oyster, Crassostrea virginica. In order to simulate the in vivo environment more closely, a chemically defined medium (JL-ODRP-3) was supplemented with tissue homogenate extracts or plasma from oysters possessing varying degrees of susceptibility to P. marinus infection. In media supplemented with extracts from highly susceptible oysters (C. virginica), P. marinus cells secreted elevated amounts of a set of low molecular weight serine proteases (LMP: 30–45 kDa) as assessed by enhanced digestion within gelatin-substrate SDS–PAGE gels. Oyster species of low susceptibility (C. gigas and C. ariakensis) did not exhibit this ability to upregulate P. marinus LMP expression. Oyster extract supplementation also led to pronounced changes in P. marinus cellular morphology, such that the cells were comparable to those observed within naturally infected oysters.

First record of Stenostomum sphagnetorum (Platyhelminthes, Catenulida) from India

Zootaxa ◽  
2020 ◽  
Vol 4816 (3) ◽  
pp. 392-396
Author(s):  
DAIZY BHARTI ◽  
FRANCISCO BRUSA ◽  
SANTOSH KUMAR ◽  
KAILASH CHANDRA
Keyword(s):  
North America ◽  
South America ◽  
Marine Species ◽  
First Record ◽  
Freshwater Ecosystems ◽  
Ganga River ◽  
Knowledge Gap ◽  
Free Living ◽  
First Report ◽  
The World

Catenulida are mostly inhabitants of freshwater ecosystems, like ponds, streams, though the marine species are few (Larsson and Willems, 2010). About 110 species of catenulids are known worldwide, with most of the studies conducted in South America (Marcus, 1945a, 1945b; Noreña et al., 2005), North America (Kepner and Carter 1931; Nuttycombe and Waters, 1938) and Scandinavian Peninsula (Luther, 1960, Larsson and Willems, 2010; Larsson et al., 2008). The diversity of catenulids from India has not been studied intensively; however some reports on other turbellaria exists for the country (Annandale, 1912; Whitehouse, 1913; Kapadia, 1947; Basil and Fernando, 1975; Apte and Pitale, 2011; Kalita and Goswami, 2012; Venkataraman et al., 2015). The genus Stenostomum, however, has been studied extensively around the world with identification of over 60 species (Tyler et al., 2006-2016). This is first report of the genus from India. The present study was part of the project to catalogue the diversity of free living protozoan ciliates from the Hooghly stretch of the Ganga River during which the flatworms were found. The worms were studied based on the live observations, with recognition of characters which led to its identification. This study serves to fill knowledge gap in the freshwater flatworms from India. 

scholarly journals Hemorrhagic cerebral metastasis as a first manifestation of a hepatocellular carcinoma: case report

1998 ◽  
Vol 56 (3B) ◽  
pp. 658-660 ◽  
Author(s):  
MARIO FERNANDO PRIETO PERES ◽  
NORA MANOUKIAN FORONES ◽  
SUZANA MARIA FLEURY MALHEIROS ◽  
HENRIQUE BALLALAI FERRAZ ◽  
JOÃO NORBERTO STÁVALE ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Case Report ◽  
South America ◽  
Cerebral Metastasis ◽  
Initial Manifestation ◽  
First Report ◽  
Rare Instance

We report herein a rare instance in which a patient presented with a hemorrhagic cerebral metastasis as the initial manifestation of a hepatocellular carcinoma (HCC). A few cases of cerebral metastasis from HCC have been reported in the literature, mainly from eastern countries. This is the first report from South America of a cerebral metastasis from hepatocellular carcinoma.

First report of plasmid-mediated fluoroquinolone efflux pump QepA in Escherichia coli clinical isolate ST68, in South America

2014 ◽  
Vol 79 (1) ◽  
pp. 70-72 ◽  
Author(s):  
Giovanna Rincón ◽  
Marcela Radice ◽  
Marta Giovanakis ◽  
Jose A. Di Conza ◽  
Gabriel Gutkind
Keyword(s):  
Escherichia Coli ◽  
South America ◽  
Clinical Isolate ◽  
Efflux Pump ◽  
First Report

scholarly journals First report of Cryptosporidium spp. oocysts in oysters (Crassostrea rhizophorae) and cockles (Tivela mactroides) in Brazil

2008 ◽  
Vol 6 (4) ◽  
pp. 527-532 ◽  
Author(s):  
Diego Averaldo Guiguet Leal ◽  
Mirna Aparecida Pereira ◽  
Regina Maura Bueno Franco ◽  
Nilson Branco ◽  
RomeuCantusio Neto
Keyword(s):  
Hepatitis A Virus ◽  
Cryptosporidium Oocyst ◽  
Coastal Region ◽  
Tween 80 ◽  
Public Health Importance ◽  
First Report ◽  
Cryptosporidium Oocysts ◽  
Thermotolerant Coliforms ◽  
Seawater Quality ◽  
Crassostrea Rhizophorae

The consumption of oysters and cockles, which are usually eaten raw or lightly-cooked, can cause outbreaks of human diseases, especially if these shellfish are harvested from polluted areas. In Brazil data about the occurrence of pathogens, like hepatitis A virus, in shellfish have been reported but research on natural contamination for pathogenic protozoa is still non-existent. Cryptosporidium oocyst contamination of oysters (Crassostrea rhizophorae) and cockles (Tivela mactroides) was evaluated during two different periods in a coastal area from São Paulo, Brazil. From June to November 2005, and from July to December 2006, 180 mollusks were harvested for tissue examination. The gills and gastrointestinal tract (n = 36 pools) were carefully extracted from the animals and homogenized in a tissue homogenizer by adding surfactant Tween 80 (0.1%). Immunofluorescence assays were performed and Cryptosporidium oocysts were detected in 50.0% of gill pools of cockles and 10.0% of gill pools of oysters. In order to evaluate seawater quality in shellfish growing areas, total levels of thermotolerant coliforms, Escherichia coli and enterococci were determined. This is the first time that Cryptosporidium oocysts were found in shellfish from the coastal region of Brazil, and to the best of our knowledge it is also the first report in Latin America and the case might be of public health importance, reflecting the extension of the contamination on seafood, requiring a need for quality control standards.

First report of equine Pegivirus in South America, Brazil

Acta Tropica ◽  
2015 ◽  
Vol 152 ◽  
pp. 56-59 ◽  
Author(s):  
Alex Junior Souza de Souza ◽  
Andreza Pinheiro Malheiros ◽  
Erika Rocha Paraense de Sousa ◽  
Alba Cristina Negrão Moreira ◽  
Andrea Lima Silva ◽  
...  
Keyword(s):  
South America ◽  
First Report
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