scholarly journals Reproducibility of intravascular ultrasound radiofrequency data analysis (virtual histology) with a 45-MHz rotational imaging catheter in ex vivo human coronary arteries

2015 ◽  
Vol 65 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Takashi Muramatsu ◽  
Hector M. García-García ◽  
Salvatore Brugaletta ◽  
Jung Ho Heo ◽  
Yoshinobu Onuma ◽  
...  
Keyword(s):  
Data Analysis ◽  
Intravascular Ultrasound ◽  
Coronary Arteries ◽  
Ex Vivo ◽  
Virtual Histology

Impact of Intramural Thrombus in Coronary Arteries on the Accuracy of Tissue Characterization by In Vivo Intravascular Ultrasound Radiofrequency Data Analysis

2008 ◽  
Vol 101 (8) ◽  
pp. 1079-1083 ◽  
Author(s):  
Kenya Nasu ◽  
Etsuo Tsuchikane ◽  
Osamu Katoh ◽  
D. Geoffrey Vince ◽  
Pauliina M. Margolis ◽  
...  
Keyword(s):  
Data Analysis ◽  
Intravascular Ultrasound ◽  
Coronary Arteries ◽  
Tissue Characterization

A Novel Experimental Method to Estimate Stress-Strain Behavior of Intact Coronary Arteries Using Intravascular Ultrasound (IVUS)

2003 ◽  
Vol 125 (1) ◽  
pp. 120-123 ◽  
Author(s):  
Azita Tajaddini ◽  
Deborah L. Kilpatrick ◽  
D. Geoffrey Vince
Keyword(s):  
Intravascular Ultrasound ◽  
Coronary Arteries ◽  
Vascular Tone ◽  
Ex Vivo ◽  
Pressure Fluctuations ◽  
Coronary Vessels ◽  
Local Pressure ◽  
Strain Behavior ◽  
Further Development

Most arterial mechanics studies have focused on excised non-coronary vessels, with few studies validating the application of ex-vivo results to in-vivo conditions. A method was developed for testing the mechanical properties of intact left anterior descending coronary arteries under a variety of conditions. Vascular deformation and pressure were simultaneously measured with intravascular ultrasound and a pressure transducer guidewire, respectively. Results suggest the importance of understanding in-vivo factors such as myocardial support, vascular tone and local pressure fluctuations when applying ex-vivo coronary characterization data. With further development, this method can more accurately characterize the true in-vivo constitutive behavior in normal and atherosclerotic coronaries.

VIRTUAL HISTOLOGY–INTRAVASCULAR ULTRASOUND IN ASSESSMENT OF CAROTID PLAQUES

Neurosurgery ◽  
2009 ◽  
Vol 65 (1) ◽  
pp. 146-152 ◽  
Author(s):  
Tomohito Hishikawa ◽  
Koji Iihara ◽  
Hatsue Ishibashi-Ueda ◽  
Kazuyuki Nagatsuka ◽  
Naoaki Yamada ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Ex Vivo ◽  
Necrotic Core ◽  
Interquartile Range ◽  
Intraplaque Hemorrhage ◽  
Virtual Histology ◽  
Carotid Plaques ◽  
Virtual Histology Intravascular Ultrasound ◽  
Histological Sections ◽  
Histological Images

Abstract OBJECTIVE Virtual histology–intravascular ultrasound (VH-IVUS) has been reported to be useful in detecting the components of coronary plaques in vivo. Recently, the application of VH-IVUS to peripheral interventions has been evaluated. The aim of this study was to examine the extent to which the necrotic core of carotid plaques could be assessed accurately by VH-IVUS compared with histopathology. METHODS A total of 37 carotid plaques underwent ex vivo VH-IVUS within 24 hours after endarterectomy. Ninety-five segments of virtual histological images were matched to histological sections. The area of the necrotic core on histological sections was compared with that on virtual histological images. Intraplaque hemorrhage (IPH) was histopathologically graded by its severity using immunohistochemical staining for glycophorin A as a marker. The relationship of the severity of the IPH to the necrotic core was histopathologically evaluated. The correlation between the necrotic core or IPH with symptomatology was also evaluated. RESULTS The area of the necrotic core on virtual histological images (median, 8.0%; interquartile range, 5.0%–13%) was significantly smaller compared with that of the histological sections (median, 50%; interquartile range, 40%–63%) (P < 0.0001). The Bland-Altman analysis showed poor agreement in the necrotic core measurement between virtual histological images and histological sections (mean difference, 39.8%; 95% confidence interval, 35.8%–43.8%). Severe IPH was significantly associated with a larger necrotic core and symptomatology (P < 0.0001 and P = 0.0039, respectively). The area of necrotic core on the virtual histological analysis did not correlate with symptomatology (P = 0.70), but that on pathological analysis tended to correlate with symptomatology (P = 0.059). CONCLUSION In the present virtual histological algorithm, the underestimation of the necrotic core was revealed. The lack of a hemorrhage component in the virtual histological algorithm is a leading cause of its underestimation.

Abstract 1984: Histopathologic Validation of Virtual Histology Intravascular Ultrasound Analysis of Peri-calcific Necrotic Core Area

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Sang-Wook Kim ◽  
Gary S Mintz ◽  
Wang-Soo Lee ◽  
Hyoung-Joong Kim ◽  
Tae-Jin Lee ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Intravascular Ultrasound ◽  
Coronary Arteries ◽  
Smooth Muscle Actin ◽  
Empty Space ◽  
Necrotic Core ◽  
Core Area ◽  
Virtual Histology ◽  
Virtual Histology Intravascular Ultrasound ◽  
Collagenous Tissue

The effect of calcium-associated acoustic shadowing on Virtual Histology intravascular ultrasound (VH-IVUS) plaque component analysis is unknown. Methods. We harvested 87 coronary arteries from 30 autopsied specimens. IVUS imaging was performed 50mm from the LMCA ostium. We then selected 10 calcified segments for comparative VH-IVUS vs pathologic comparisons. Results. Pt age was 46±8.9yrs and 90% were males. VH-IVUS analysis of the calcified plaque showed 11±3.8% necrotic core, 8±1.9% fibrofatty plaque, 69±9.9% fibrotic plaque, and 10±6.6% dense calcium. Dense calcium was correlated with the size of the necrotic core (r=0.774, p=0.009). Three coronary arteries with moderate to severe calcium were examined histopathologically. VH-IVUS analysis showed fibrotic tissue behind the calcium, but histopathology showed an acellular sclerotic area (dense collagenous tissue) just beneath the calcium with a relatively well-preserved vascular smooth muscle layer. Within the necrotic core, VH-IVUS showed prominent red necrotic core area combined with calcium; however, histopathology showed empty space of decalcification surrounded by just collagenous tissue with outer smooth muscle actin positive cell layer. More importantly, no active inflammation was present around calcium. Conclusion. VH-IVUS necrotic core surrounding areas of dense calcium correlated with dense collagenous tissue histopathologically. It is important not to overinterpret VH IVUS studies of dense calcium with prominent necrotic core. More precise classification/validation for necrotic core is necessary.

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