Highly-selective detection of EGFR mutation gene in lung cancer based on surface enhanced Raman spectroscopy and asymmetric PCR

2020 ◽  
Vol 190 ◽  
pp. 113522
Author(s):  
Ting Guo ◽  
Weimiao Li ◽  
Lu Qian ◽  
Xiaolong Yan ◽  
Daxiang Cui ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Egfr Mutation ◽  
Surface Enhanced Raman Spectroscopy ◽  
Selective Detection ◽  
Asymmetric Pcr ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Xiaowei Cao ◽  
Zhenyu Wang ◽  
Liyan Bi ◽  
Jie Zheng
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Cell Lung Cancer ◽  
Surface Enhanced Raman Spectroscopy ◽  
Small Cell ◽  
Label Free ◽  
Small Cell Lung ◽  
Surface Enhanced ◽  
Different Types ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) is a good candidate for the development of fast and easy-to-use diagnostic tools, possibly used on serum in screening tests. In this study, a potential label-free serum test based on SERS spectroscopy was developed to analyze human serum for the diagnosis of the non-small cell lung cancer (NSCLC). We firstly synthesized novel highly branched gold nanoparticles (HGNPs) at high yield through a one-step reduction of HAuCl4 with dopamine hydrochloride at 60°C. Then, HGNP substrates with good reproducibility, uniformity, and high SERS effect were fabricated by the electrostatically assisted (3-aminopropyl) triethoxysilane-(APTES-) functionalized silicon wafer surface-sedimentary self-assembly method. Using as-prepared HGNP substrates as a high-performance sensing platform, SERS spectral data of serum obtained from healthy subjects, lung adenocarcinoma patients, lung squamous carcinoma patients, and large cell lung cancer patients were collected. The difference spectra among different types of NSCLC were compared, and analysis result revealed their intrinsic difference in types and contents of nucleic acids, proteins, carbohydrates, amino acids, and lipids. SERS spectra were analyzed by principal component analysis (PCA), which was able to distinguish different types of NSCLC. Considering its time efficiency, being label-free, and sensitivity, SERS based on HGNP substrates is very promising for mass screening NSCLC and plays an important role in the detection and prevention of other diseases.

scholarly journals Sensitive and Selective Detection of New Red Colorant Based on Surface-Enhanced Raman Spectroscopy Using Molecularly Imprinted Hydrogels

2019 ◽  
Vol 9 (13) ◽  
pp. 2672 ◽  
Author(s):  
Jing Neng ◽  
Kaiyun Xu ◽  
Yan Wang ◽  
Kan Jia ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Selective Detection ◽  
Molecularly Imprinted ◽  
Au Nps ◽  
Relative Standard ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Active Substrate ◽  
Positively Charged

A polyacrylamide-based molecularly imprinted hydrogel (MIH) doped with positively charged gold nanoparticles (Au NPs) has been synthesized via a free radical polymerization of acrylamide (AM) aqueous solution containing positively charged Au NPs as a Raman active substrate, New Red colorant as a template molecule, N,N’-methylenebis(acrylamide) as a crosslinking agent, and potassium persulfate as an initiator. The Au NPs-doped MIHs were subsequently explored as a Raman active substrate for the sensitive and selective detection of New Red colorant via surface-enhanced Raman spectroscopy (SERS). The logarithmic intensity of the characteristic peak of New Red at 1572 cm−1 was proportional to the logarithmic concentration of New Red with a detection linear range of 1.64 × 10−6 to 1.64 × 10−4 M and a limit of detection (LOD) of 1.64 × 10−7 M. The recoveries ranged from 86.3% to 100.6% with a relative standard deviation (RSD) in the range of 2.3% to 7.7%. The RSD and recovery rates for the detection of New Red spiked in a sports drink sample were 1.8% to 7.7% and 91.0% to 97.1%, respectively. These results showed that SERS combined with MIHs as Raman active substrates could provide a sensitive, selective, and effective approach for the detection of the New Red colorant in beverage matrix.

Combination assay of lung cancer associated serum markers using surface-enhanced Raman spectroscopy

2016 ◽  
Vol 4 (10) ◽  
pp. 1811-1817 ◽  
Author(s):  
Chunyuan Song ◽  
Yanjun Yang ◽  
Boyue Yang ◽  
Linghua Min ◽  
Lianhui Wang
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Human Serum ◽  
Surface Enhanced Raman Spectroscopy ◽  
Serum Markers ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A highly sensitive and specific SERS-based combination assay of lung cancer associated serum markers is reported, and the LODs of CEA and NSE in human serum specimens are 1.48 pg mL−1 and 2.04 pg mL−1, respectively.

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