Fractionated illumination after topical application of 5-aminolevulinic acid on normal skin of hairless mice: The influence of the dark interval

Aim. To determine whether 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is effective in combating ultraviolet A- (UVA-) induced oxidative photodamage of hairless mice skin in vivo and human epidermal keratinocytes in vitro. Methods. In in vitro experiments, the human keratinocyte cell line (HaCaT cells) was divided into two groups: the experimental group was treated with ALA-PDT and the control group was left untreated. Then, the experimental group and the control group of cells were exposed to 10 J/m2 of UVA radiation. ROS, O2− species, and MMP were determined by fluorescence microscopy; p53, OGG1, and XPC were determined by Western blot analysis; apoptosis was determined by flow cytometry; and 8-oxo-dG was determined by immunofluorescence. Moreover, HaCaT cells were also treated with ALA-PDT. Then, SOD1 and SOD2 were examined by Western blot analysis. In in vivo experiments, the dorsal skin of hairless mice was treated with ALA-PDT or saline-PDT, and then, they were exposed to 20 J/m2 UVA light. The compound 8-oxo-dG was detected by immunofluorescence. Conclusion. In human epidermal keratinocytes and hairless mice skin, UVA-induced oxidative damage can be prevented effectively with ALA-PDT pretreatment.

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Quantitative and qualitative analysis of endogenous porphyrin accumulation in epithelial tumors after topical application of delta-aminolevulinic acid in vivo

Journal of Dermatological Science ◽

10.1016/s0923-1811(98)84345-4 ◽

1998 ◽

Vol 16 ◽

pp. S225

Author(s):

Sonja Radakovic ◽

Reinhard Konschitzky ◽

Herbert Hönigsmann ◽

Adrian Tanew

Keyword(s):

Qualitative Analysis ◽

Topical Application ◽

Aminolevulinic Acid ◽

Epithelial Tumors

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Study of Protoporphyrin IX (PpIX) Pharmacokinetics After Topical Application of 5-Aminolevulinic Acid in Urethral Condylomata Acuminata

Photochemistry and Photobiology ◽

10.1111/j.1751-1097.2007.00178.x ◽

2007 ◽

Vol 83 (5) ◽

pp. 1069-1073 ◽

Cited By ~ 25

Author(s):

Xiu-Li Wang ◽

Hong-Wei Wang ◽

Zheng Huang ◽

Herbert Stepp ◽

Reinhold Baumgartner ◽

...

Keyword(s):

Topical Application ◽

Aminolevulinic Acid ◽

Protoporphyrin Ix ◽

Condylomata Acuminata

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Use of digital photography to identify neoplastic skin lesions after labelling by ALA-derived protoporphyrin

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424621500309 ◽

2021 ◽

pp. A-G

Author(s):

Hyunseon Yu ◽

Tien Son Ho ◽

Heesung Kang ◽

Youngwoo Bae ◽

Eung Ho Choi ◽

...

Keyword(s):

Actinic Keratosis ◽

Aminolevulinic Acid ◽

Imaging Modality ◽

Normal Skin ◽

Protoporphyrin Ix ◽

Image Data ◽

Skin Area ◽

Clinical Images ◽

Actinic Keratosis Lesion ◽

Fluorescence Images

Actinic keratosis is a premalignant skin lesion that develops into non-melanoma skin cancer. Various imaging techniques have been developed to find the actinic keratosis lesion. In this clinical study, the feasibility of a nonspectroscopic fluorescence imaging system is investigated for spatial assessment of the actinic keratosis lesion. Six patients between the ages of 70 and 80 years old are diagnosed with actinic keratosis by a board-certified dermatologist to obtain biopsy-proven clinical images. The patients were treated with 5-aminolevulinic acid, which is transformed into the protoporphyrin IX. After illuminating ultraviolet-A light on facial lesions, the protoporphyrin IX produces the exogenous fluorescence. The fluorescence is measured using both a hyperspectral camera and an RGB color camera to obtain spectroscopic and nonspectroscopic fluorescence images, respectively. It is found that fluorescence intensity of the actinic keratosis lesion is higher than that of normal skin. Based on combined fluorescence and physiological characteristics, the actinic keratosis lesion is distinguished from the adjacent normal skin area. For delineation of the actinic keratosis lesion, a linear unmixing algorithm is applied to spectroscopic image data and an erythema index is calculated from nonspectroscopic image data. Then, two extracted actinic keratosis lesions are compared for cross-validation. As a result, both spectroscopic and nonspectroscopic fluorescence images demarcate an identical lesion of actinic keratosis. Given the affordability and simplicity, an RGB camera and a 5-ALA photosensitizer can be used as a cost-effective nonspectroscopic imaging modality for accurate assessment of actinic keratosis margins.

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