Intracellular signaling mechanisms mediating the antiproliferative and apoptotic effects of γ-tocotrienol in neoplastic mammary epithelial cells

2005 ◽  
Vol 162 (7) ◽  
pp. 803-810 ◽  
Author(s):  
Paul W. Sylvester ◽  
Sumit J. Shah ◽  
Ganesh V. Samant
Keyword(s):  
Epithelial Cells ◽  
Intracellular Signaling ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Signaling Mechanisms ◽  
Apoptotic Effects

Antiproliferative and Apoptotic Effects of Tocopherols and Tocotrienols on Preneoplastic and Neoplastic Mouse Mammary Epithelial Cells

2000 ◽  
Vol 224 (4) ◽  
pp. 292-301 ◽  
Author(s):  
Barry S. McIntyre ◽  
Karen P. Briski ◽  
Abdul Gapor ◽  
Paul W. Sylvester
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Apoptotic Effects

Antiproliferative and apoptotic effects of tocopherols and tocotrienols on normal mouse mammary epithelial cells

Lipids ◽  
2000 ◽  
Vol 35 (2) ◽  
pp. 171-180 ◽  
Author(s):  
Barry S. McIntyre ◽  
Karen P. Briski ◽  
Mark A. Tirmenstein ◽  
Marc W. Fariss ◽  
Abdul Gapor ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Normal Mouse ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Apoptotic Effects

Antiproliferative and Apoptotic Effects of Tocopherols and Tocotrienols on Preneoplastic and Neoplastic Mouse Mammary Epithelial Cells

2008 ◽  
Vol 224 (4) ◽  
pp. 292-301 ◽  
Author(s):  
Barry S. McIntyre ◽  
Karen P. Briski ◽  
Abdul Gapor ◽  
Paul W. Sylvester
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Apoptotic Effects

scholarly journals Transcriptional Repression of MFG-E8 Causes Disturbance in the Homeostasis of Cell Cycle Through DOCK/ZP4/STAT Signaling in Buffalo Mammary Epithelial Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Arvind K. Verma ◽  
Syed A. Ali ◽  
Parul Singh ◽  
Sudarshan Kumar ◽  
Ashok K. Mohanty
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Epithelial Cells ◽  
Intracellular Signaling ◽  
Molecular Mechanisms ◽  
Mammary Epithelial Cells ◽  
Bovine Milk ◽  
Mammary Epithelial ◽  
Directed Network ◽  
Ideal System

The mammary gland is a unique apocrine gland made up of a branching network of ducts that end in alveoli. It is an ideal system to study the molecular mechanisms associated with cell proliferation, differentiation, and oncogenesis. MFG-E8, also known as Lactadherin, is a vital glycoprotein related to the milk fat globule membrane and initially identified to get secreted in bovine milk. Our previous report suggests that a high level of MFG-E8 is indicative of high milk yield in dairy animals. Here, we showed that MFG-E8 controls the cell growth and morphology of epithelial cells through a network of regulatory transcription factors. To understand the comprehensive action, we downregulated its expression in MECs by MFG-E8 specific shRNA. We generated a knockdown proteome profile of differentially expressed proteins through a quantitative iTRAQ experiment on a high-resolution mass spectrometer (Q-TOF). The downregulation of MFG-E8 resulted in reduced phagocytosis and cell migration ability, whereas it also leads to more lifespan to knockdown vis-a-vis healthy cells, which is confirmed through BrdU, MTT, and Caspase 3/7. The bioinformatics analysis revealed that MFG-E8 knockdown perturbs a large number of intracellular signaling, eventually leading to cessation in cell growth. Based on the directed network analysis, we found that MFG-E8 is activated by CX3CL1, TP63, and CSF2 and leads to the activation of SOCS3 and CCL2 for the regulation of cell proliferation. We further proved that the depletion of MFG-E8 resulted in activated cytoskeletal remodeling by MFG-E8 knockdown, which results in the activation of three independent pathways ZP4/JAK-STAT5, DOCK1/STAT3, and PIP3/AKT/mTOR. Overall, this study suggests that MFG-E8 expression in mammary epithelial cells is an indication of intracellular deterioration in cell health. To date, to the best of our knowledge, this is the first study that explores the downstream targets of MFG-E8 involved in the regulation of mammary epithelial cell health.

scholarly journals Integrin Signaling in Mammary Epithelial Cells and Breast Cancer

ISRN Oncology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Arthur W. Lambert ◽  
Sait Ozturk ◽  
Sam Thiagalingam
Keyword(s):  
Breast Cancer ◽  
Epithelial Cells ◽  
Intracellular Signaling ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Integrin Signaling ◽  
Normal Mammary Gland ◽  
Integrin Receptors ◽  
Sense And Respond

Cells sense and respond to the extracellular matrix (ECM) by way of integrin receptors, which facilitate cell adhesion and intracellular signaling. Advances in understanding the mammary epithelial cell hierarchy are converging with new developments that reveal how integrins regulate the normal mammary gland. But in breast cancer, integrin signaling contributes to the development and progression of tumors. This paper highlights recent studies which examine the role of integrin signaling in mammary epithelial cells and their malignant counterparts.

Human mammary epithelial cells are sensitized to ionizing radiation by knockdown of caveolin-1

2010 ◽  
Vol 34 (8) ◽  
pp. S7-S7
Author(s):  
Nan Zhang ◽  
Jian Che ◽  
Zheng Wu ◽  
Yang Wang ◽  
Liwei Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ionizing Radiation ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Caveolin 1 ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial

Microphysiometry to Evaluate Real-Time Response of Mammary Epithelial Cells to IGF-I

2000 ◽  
Vol 28 (1-2) ◽  
pp. 209-211 ◽  
Author(s):  
Rose Marie Robinson ◽  
R. Michael Akers ◽  
Kimberly E. Forsten
Keyword(s):  
Epithelial Cells ◽  
Real Time ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Time Response ◽  
Igf I
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