Quantitative proteomic analysis of Neosartorya pseudofischeri ascospores subjected to heat treatment

2021 ◽  
pp. 104446
Si Chen ◽  
Lihua Fan ◽  
Jun Song ◽  
Hong Zhang ◽  
Craig Doucette ◽  
2021 ◽  
Vol 135 ◽  
pp. 204-216
Dingding Lü ◽  
Ping Xu ◽  
Chengxiang Hou ◽  
Ruilin Li ◽  
Congwu Hu ◽  

2021 ◽  
Vol 19 (1) ◽  
Huiyi Song ◽  
Ni Lou ◽  
Jianjun Liu ◽  
Hong Xiang ◽  
Dong Shang

Abstract Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance. This study employed proteomic analysis to identify differentially expressed proteins after coculture of E. coli with Lactobacillus rhamnosus GG (LGG) microcapsules. Methods To explore the relevant protein abundance changes after E. coli and LGG coculture, label-free quantitative proteomic analysis and qRT-PCR were applied to E. coli and LGG microcapsule groups before and after coculture, respectively. Results The proteomic analysis characterised a total of 1655 proteins in E. coli K12MG1655 and 1431 proteins in the LGG. After coculture treatment, there were 262 differentially expressed proteins in E. coli and 291 in LGG. Gene ontology analysis showed that the differentially expressed proteins were mainly related to cellular metabolism, the stress response, transcription and the cell membrane. A protein interaction network and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. Conclusions These findings indicated that LGG microcapsules may inhibit E. coli biofilm formation by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

2013 ◽  
Vol 71 (Suppl 3) ◽  
pp. 160.3-160
P. Fernández-Puente ◽  
L. Lourido ◽  
V. Calamia ◽  
J. Mateos ◽  
C. Ruiz-Romero ◽  

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