Tilt-series and electron microscope alignment for the correction of the non-perpendicularity of beam and tilt-axis

2006 ◽  
Vol 154 (2) ◽  
pp. 195-205 ◽  
Author(s):  
Daniel Castaño Díez ◽  
Anja Seybert ◽  
Achilleas S. Frangakis
Keyword(s):  
Electron Microscope ◽  
Tilt Axis ◽  
Tilt Series

Transform-based backprojection for volume reconstruction of large format electron microscope tilt series

2006 ◽  
Vol 154 (2) ◽  
pp. 144-167 ◽  
Author(s):  
Albert Lawrence ◽  
James C. Bouwer ◽  
Guy Perkins ◽  
Mark H. Ellisman
Keyword(s):  
Electron Microscope ◽  
Volume Reconstruction ◽  
Large Format ◽  
Tilt Series

scholarly journals Automated Functions in Electron Microscopy

2004 ◽  
Vol 12 (6) ◽  
pp. 14-19
Author(s):  
Bill Tivol
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Image Feature ◽  
Tilt Axis ◽  
Rotation Center ◽  
Computer Controlled ◽  
Electron Microscopes ◽  
Automated Procedures

The newest generation of computer-controlled electron microscopes incorporates the ability to perform adjustments to microscopy conditions by comparing pairs of images and altering the conditions accordingly. Automation of electron microscope adjustments offers the advantages of accuracy, precision, efficiency, the ability to incorporate the adjustments into other automated procedures, and, for radiation-sensitive specimens, minimal exposure to the beam.At present, automated functions include determination of eucentric height, focus, astigmatism, orientation and location of the stage tilt axis, centering of an image feature, and rotation center alignment. It is possible to automate other functions, so this list may be incomplete. In general, these functions are accomplished by induced image shifts, and, in many cases, the automated functions are completely analogous to the corresponding manual ones.

A Method for Observing Silver-Stained Osteocytes In Situ in 3-μm Sections Using Ultra-High Voltage Electron Microscopy Tomography

2009 ◽  
Vol 15 (5) ◽  
pp. 377-383 ◽  
Author(s):  
Hiroshi Kamioka ◽  
Sakhr A. Murshid ◽  
Yoshihito Ishihara ◽  
Naoko Kajimura ◽  
Toshiaki Hasegawa ◽  
...  
Keyword(s):  
Electron Microscope ◽  
High Voltage ◽  
Morphological Data ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Specimen Holder ◽  
High Voltage Electron ◽  
Tilt Series ◽  
Ultra High Voltage

AbstractOsteocytes are surrounded by hard bone matrix, and it has not been possible previously to directly observe the in situ architecture of osteocyte morphology in bone. Electron microscope tomography, however, is a technique that has the unique potential to provide three-dimensional (3D) visualization of cellular ultrastructure. This approach is based on reconstruction of 3D volumes from a tilt series of electron micrographs of cells, and resolution at the nanometer level has been achieved. We applied electron microscope tomography to thick sections of silver-stained osteocytes in bone using a Hitachi H-3000 ultra-high voltage electron microscope equipped with a 360° tilt specimen holder, at an accelerating voltage of 2 MeV. Osteocytes with numerous processes and branches were clearly seen in the serial tilt series acquired from 3-μm-thick sections. Reconstruction of young osteocytes showed the 3D topographic morphology of the cell body and processes at high resolution. This morphological data on osteocytes should provide useful information to those who study osteocyte physiology and the several models used to explain their mechanosensory properties.

Fast auto-acquisition tomography tilt series by using HD video camera in ultra-high voltage electron microscope

Microscopy ◽  
2014 ◽  
Vol 63 (suppl 1) ◽  
pp. i25.1-i25 ◽  
Author(s):  
Ryuji Nishi ◽  
Meng Cao ◽  
Atsuko Kanaji ◽  
Tomoki Nishida ◽  
Kiyokazu Yoshida ◽  
...  
Keyword(s):  
Electron Microscope ◽  
High Voltage ◽  
Video Camera ◽  
Voltage Electron ◽  
High Voltage Electron Microscope ◽  
High Voltage Electron ◽  
Tilt Series ◽  
Ultra High Voltage

Electron Cytochemical Study of Carbon Dioxide Laser Effect on Rhesus Monkey Cornea

1974 ◽  
Vol 32 ◽  
pp. 224-225
Author(s):  
K. C. Tsou ◽  
J. Morris ◽  
P. Shawaluk ◽  
B. Stuck ◽  
E. Beatrice
Keyword(s):  
Carbon Dioxide ◽  
Electron Microscope ◽  
Rhesus Monkey ◽  
Carbon Dioxide Laser ◽  
Cytochemical Study ◽  
Laser Effect

While much is known regarding the effect of lasers on the retina, little study has been done on the effect of lasers on cornea, because of the limitation of the size of the material. Using a combination of electron microscope and several newly developed cytochemical methods, the effect of laser can now be studied on eye for the purpose of correlating functional and morphological damage. The present paper illustrates such study with CO2 laser on Rhesus monkey.

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