Performance of cowpea weevil Callosobruchus maculatus (F.) infesting seeds of different Vigna unguiculata (L.) Walpers genotypes: The association between bruchid resistance and chitin binding proteins

2022 ◽  
Vol 95 ◽  
pp. 101925
Author(s):  
Kayan Eudorico Ventury ◽  
Sarah Rodrigues Ferreira ◽  
Maurisrael de Moura Rocha ◽  
Geraldo do Amaral Gravina ◽  
André Teixeira da Silva Ferreira ◽  
...  
2014 ◽  
Vol 13 (1) ◽  
pp. 2323-2332 ◽  
Author(s):  
M.X. Vila Nova ◽  
N.G.A. Leite ◽  
L.M. Houllou ◽  
L.V. Medeiros ◽  
A.C. Lira Neto ◽  
...  

2017 ◽  
Vol 10 (1) ◽  
pp. 19-25
Author(s):  
Westerllanya Rodrigues Medeiros ◽  
Jayara Dayany da Costa Silva ◽  
Paulo Roberto Ramalho Silva ◽  
José Edmir Girão Filho ◽  
Luiz Evaldo de Moura Padua ◽  
...  

Resumo. O caruncho Callosobruchus maculatus (Fabr.) é considerado a principal praga durante o armazenamento de grãos de feijão-caupi [Vigna unguiculata(L.) Walp.]. Devido aos prejuízos econômicos causados por este inseto, é de suma importância que se desenvolvam estudos que selecionem variedades resistentes a essa praga. Neste trabalho avaliou-se o efeito de genótipos de V. unguiculata sobre o comportamento e o desenvolvimento de C. maculatus em duas gerações consecutivas.  O trabalho foi conduzido em Laboratório com condições de temperatura e umidade relativa monitoradas, em delineamento inteiramente casualizado utilizando os genótipos BRS Epace 10, Capela, BRS Urubuquara, BRS Pajeu, Itaim, BRS Rouxinol, Pingo de ouro, BRS Corujinha, IT85 F-2687 e BR 17 Gurguéia com oito repetições. Em cada genótipo foram confinados 10 adultos de C. maculatus para que fosse realizada a oviposição. Após um período de cinco dias, os adultos foram retirados dos potes contendo os genótipos. Aguardou-se a emergência dos adultos (primeira geração) e realizou-se nova infestação nos mesmos acessos para obtenção da segunda geração. Os parâmetros avaliados foram número de ovos total, viabilidade de ovos, número de adultos emergidos, viabilidade da fase imatura, período médio de desenvolvimento e peso seco dos adultos.  O genótipo IT85 F2687 apresentou resistência do tipo não-preferência para oviposição e os genótipos BR 17 Gurguéia e BRS Urubuquara apresentaram resistência do tipo antibiose em relação à C. maculatus. O genótipo BR 17 Gurguéia foi o mais resistente dentre os genótipos estudados em relação à C. maculatus. Os genótipos Capela e Itaim foram caracterizados como suscetíveis a C. maculatus.Resistance of cowpea [Vigna unguiculata (L.) Walp.] genotypes to weevil attack Callosobruchus maculatus (Fabr.) (Coleoptera: Chrysomelidae)Abstract. The Cowpea weevil Callosobruchus maculatus (Fabr.) is considered the main Prague during storage of grains of Cowpea [Vigna unguiculata (L.) Walp.]. Due to the economic losses caused by this insect, is of paramount importance to develop studies to select varieties resistant to this pest. In this study assessed the effect of genotypes of V. unguiculata about the behavior and the development of C. maculatus in two consecutive generations. The study was conducted in laboratory with conditions of temperature and relative humidity monitored, in completely randomized design using BRS Epace 10, Capela, BRS Urubuquara, BRS Pajeu, Itaim, BRS Rouxinol, Pingo de ouro, BRS Corujinha, IT85 F-2687 and BR 17 Gurguéia genotypes with eight repetitions. In each genotype were confined 10 adults of c. maculatus for it was held the oviposition. After a period of five days, the adults were removed from the pots containing the genotypes. Waited-if the emergence of adults (first generation) and new infestation was held in the same access to obtain the second generation. The parameters evaluated were number of eggs, egg viability, total number of adults emergency, viability of immature phase, average period of development and dry weight of adults. IT85 F2687 genotype presented resistance of non-preference for oviposition and the genotypes BR 17 Gurguéia and BRS Urubuquara presented antibiose type resistance in relation to C. maculatus. BR 17 Gurguéia genotype was the strongest among the genotypes studied in relation to c. maculatus. The genotypes Capela and Itaim were characterized as susceptible to C. maculatus.</p


Author(s):  
Maria Lígia R. Macedo ◽  
Lúcia Betânia Da S. Andrade ◽  
Rosana A. Moraes ◽  
Jose Xavier-Filho

2002 ◽  
Vol 45 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Antônio Chagas Mota ◽  
Kátia Valevski Sales Fernandes ◽  
Maurício Pereira Sales ◽  
Victor Martin Quintana Flores ◽  
José Xavier-Filho

Vicilins (7S storage globulins) isolated from cowpea (Vigna unguiculata L.) seeds which were susceptible (S) and resistant (R) to the cowpea weevil (Callosobruchus maculatus F., Coleoptera: Bruchidae) were denatured by urea and fractionated by ion-exchange chromatography. Isolated fractions were incorporated in artificial seeds for assessment of their toxicity to C. maculatus. The most acidic fractions of both susceptible (CE-31 cultivar) and resistant (IT81D-1045 line) seeds were shown to affect development and survival of the bruchid. Results indicated that vicilin polypeptides of toxic nature were expressed in both types of storage globulins although at different levels.


1983 ◽  
Vol 34 (6) ◽  
pp. 697 ◽  
Author(s):  
R Redden

Seed from F3 generation plants of two reciprocal crosses were evaluated for bruchid resistance, over 28 F3 families of four plants each plus an additional five plants each in 10-11 families. The variables, percentage adult emergence for a specified period and mean time to emergence, agreed with respect to describing segregation in the resistant, intermediate and susceptible categories. Resistance was expressed as a recessive character. No differences between families from reciprocal crosses were detected. There was evidence for digenic control of resistance in one cross and for monogenic control in the other, in conjunction with one, or more, modifier or minor gene loci. There was general agreement between the results for the F3 generation study and a previous study on the F2 generation.


Author(s):  
Olusola Michael Obembe ◽  
Joshua Kayode

A laboratory study was conducted to investigate the efficacy of powder and extract of C. alata on Callosobruchus maculatus Fab. damage in stored cowpea (Vigna unguiculata L.) seeds. The powder and the extract were evaluated on C. maculatus for mortality, oviposition and adult emergence effects. The long-term storage of the treated seeds was also investigated. All treatments recorded higher significant (p < 0.05) mortality than the experimental control. Highest mortality of 100 % was recorded in seeds treated with C. alata extract at the dosage level of 4.0 % v/w while highest mortality of 80.30 % was also recorded in seed treated with 4.0 g of C. alata powder. The powder and the extract significantly reduced oviposition and adult emergence in the treated seed. Adult emergence was totally suppressed in seeds treated with 3.0 % v/w and 4.0 % v/w. The powder and the extract also reduced infestation and subsequence damage of the treated seeds. No damage was recorded in seeds treated with 3.0 % v/w and 4.0 % v/w of the extract. The result obtained revealed that extract and powder of C. alata were effective in controlling C. maculatus and could be recommended as an alternative to synthetic insecticides.


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