In this chapter, the authors introduce three typical applications of electronic nose, including the diagnosis of lung cancer and gastrointestinal diseases and food freshness detection. Firstly, an electronic nose system intended for lung cancer screening employs semiconductor and electrochemical sensors to compose an array in a chamber to conduct breath analysis with pre-concentration and multiple desorption procedures. Secondly, a design of virtual electronic nose was presented to detect the concentration of hydrogen, methane, and carbon dioxide in breath for the diagnosis of small intestinal bacterial overgrowth. Finally, combined with the metal oxide semiconductor sensors and electrochemical sensors array, the small size electronic nose system is to identify the freshness and classify different kinds of food stored in the refrigerator.