Optimization of mixotrophic medium components for biomass production and biochemical composition biosynthesis by Chlorella vulgaris using response surface methodology

2012 ◽  
Vol 43 (3) ◽  
pp. 360-367 ◽  
Author(s):  
Wei-Bao Kong ◽  
Shao-Feng Hua ◽  
Hai Cao ◽  
Yu-Wen Mu ◽  
Hong Yang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Biochemical Composition ◽  
Medium Components

scholarly journals Growth and biochemical composition of Chlorella vulgaris in different growth media

2013 ◽  
Vol 85 (4) ◽  
pp. 1427-1438 ◽  
Author(s):  
MATHIAS A. CHIA ◽  
ANA T. LOMBARDI ◽  
MARIA DA GRACA G. MELAO
Keyword(s):  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Biochemical Composition ◽  
Physiological Response ◽  
Low Cost ◽  
Cost Effective ◽  
Growth Conditions ◽  
Growth Media ◽  
Continuous Cultures ◽  
The Cost

The need for clean and low-cost algae production demands for investigations on algal physiological response under different growth conditions. In this research, we investigated the growth, biomass production and biochemical composition of Chlorella vulgaris using semi-continuous cultures employing three growth media (LC Oligo, Chu 10 and WC media). The highest cell density was obtained in LC Oligo, while the lowest in Chu medium. Chlorophyll a, carbohydrate and protein concentrations and yield were highest in Chu and LC Oligo media. Lipid class analysis showed that hydrocarbons (HC), sterol esthers (SE), free fatty acids (FFA), aliphatic alcohols (ALC), acetone mobile polar lipids (AMPL) and phospholipids (PL) concentrations and yields were highest in the Chu medium. Triglyceride (TAG) and sterol (ST) concentrations were highest in the LC Oligo medium. The results suggested that for cost effective cultivation, LC Oligo medium is the best choice among those studied, as it saved the cost of buying vitamins and EDTA associated with the other growth media, while at the same time resulted in the best growth performance and biomass production.

Optimising of Scenedesmus sp. biomass production in chicken slaughterhouse wastewater using response surface methodology and potential utilisation as fish feeds

2019 ◽  
Vol 26 (12) ◽  
pp. 12089-12108 ◽  
Author(s):  
Maizatul Azrina Yaakob ◽  
Radin Maya Saphira Radin Mohamed ◽  
Adel Al-Gheethi ◽  
Athirah Tiey ◽  
Amir Hashim Mohd Kassim
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Production ◽  
Slaughterhouse Wastewater ◽  
Fish Feeds

Optimization of Medium Components for β-Glucosidase Production from Aspergillus Niger by Response Surface Methodology

2013 ◽  
Vol 419 ◽  
pp. 328-333 ◽  
Author(s):  
Chao Zhang ◽  
Rui Huang ◽  
Hui Tian ◽  
Ru Ming Zhao ◽  
Fa Shun Yu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Influence Factors ◽  
Mutual Effect ◽  
Tween 80 ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Malt Extract ◽  
Medium Components

β-Glucosidase is the key enzyme for the utilization of lignocellulose.But the commercial β-glucosidase can’t be produced. This paper focuses on the study of the β-glucosidase fermentation process.The fermentation medium components for β-glucosidase production from Aspergil lusniger was optimized by response surface methodology (RSM). Firstly, the three of the most important influence factors yeast extract, MnSO4•H2O and MgSO4•7H2O was obtained from Plackett-Burman design screening. Then the path of steepest ascent experiment was adopted to approach the optimal region of the medium composition. Lastly, the optimal concentration and mutual effect of three factors were predicted by RSM. The results showed that the best medium composition was Malt extract 18g/L, Yeast extract 3.22g/L, KH2PO4 3g/L, MnSO4•H2O 0.58mM, Tween-80 0.5mL/L and MgSO4•7H2O 0.23g/L. Under these fermentation conditions, the activity of β-glucosidase was up to 7.33IU/mL with increasing 23.2% than before.

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