Modification of crosslinked chitosan beads with histidine and Saccharomyces cerevisiae for enhanced Ni(II) biosorption

2015 ◽  
Vol 56 ◽  
pp. 96-102 ◽  
Author(s):  
My Linh Nguyen ◽  
Ruey-Shin Juang
2015 ◽  
Vol 126 ◽  
pp. 97-107 ◽  
Author(s):  
Shu-Huei Yu ◽  
Shao-Jung Wu ◽  
Jui-Yu Wu ◽  
De-Yu Wen ◽  
Fwu-Long Mi

Catalysts ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1158
Author(s):  
Zhanyong Wang ◽  
Tingting Su ◽  
Jingjing Zhao

Genipin was used as a crosslinking agent to prepare magnetic genipin-crosslinked chitosan beads, which were then used as a carrier for immobilizing recombinant cutinase from Fusarium solani (FSC) to obtain immobilized FSC. The optimal temperature for the immobilized FSC was 55 °C, which was 5 °C higher than that of the free enzyme, whereas its optimal pH was increased from 8.0 to 9.0; this indicates that the immobilized FSC had improved pH and thermal stability. After repeated use for 10 cycles, the activity of the immobilized FSC remained at more than 50%; after being stored at 4 °C for 30 days, its activity was still approximately 88%. We also found that the Km of the immobilized FSC was higher than that of the free enzyme. These results indicate that the performance of FSC was improved after immobilization, which is an important basis for the subsequent application of FSC in industrial production.


2008 ◽  
Vol 43 (1) ◽  
pp. 83-92 ◽  
Author(s):  
Feng-Ming Hsieh ◽  
Chih Huang ◽  
Tsair-Fuh Lin ◽  
Yan-Min Chen ◽  
Jui-Che Lin

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