Study of sodium tripolyphosphate-crosslinked chitosan beads entrapped with Pseudomonas putida for phenol degradation

2008 ◽  
Vol 43 (1) ◽  
pp. 83-92 ◽  
Author(s):  
Feng-Ming Hsieh ◽  
Chih Huang ◽  
Tsair-Fuh Lin ◽  
Yan-Min Chen ◽  
Jui-Che Lin
2002 ◽  
Vol 184 (24) ◽  
pp. 6957-6965 ◽  
Author(s):  
Signe Saumaa ◽  
Andres Tover ◽  
Lagle Kasak ◽  
Maia Kivisaar

ABSTRACT Stationary-phase mutations occur in populations of stressed, nongrowing, and slowly growing cells and allow mutant bacteria to overcome growth barriers. Mutational processes in starving cells are different from those occurring in growing bacteria. Here, we present evidence that changes in mutational processes also take place during starvation of bacteria. Our test system for selection of mutants based on creation of functional promoters for the transcriptional activation of the phenol degradation genes pheBA in starving Pseudomonas putida enables us to study base substitutions (C-to-A or G-to-T transversions), deletions, and insertions. We observed changes in the spectrum of promoter-creating mutations during prolonged starvation of Pseudomonas putida on phenol minimal plates. One particular C-to-A transversion was the prevailing mutation in starving cells. However, with increasing time of starvation, the importance of this mutation decreased but the percentage of other types of mutations, such as 2- to 3-bp deletions, increased. The rate of transversions was markedly elevated in the P. putida MutY-defective strain. The occurrence of 2- to 3-bp deletions required the stationary-phase sigma factor RpoS, which indicates that some mutagenic pathway is positively controlled by RpoS in P. putida.


2001 ◽  
Vol 266 (2) ◽  
pp. 199-206 ◽  
Author(s):  
L. Petruschka ◽  
G. Burchhardt ◽  
C. Müller ◽  
C. Weihe ◽  
H. Herrmann

1993 ◽  
Vol 229 ◽  
pp. 181-192 ◽  
Author(s):  
Ch. Motzkus ◽  
G. Welge ◽  
I. Lamprecht

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