Effect of chitosan second layer on the gelation and controlled digestion of Citrem-chitosan bilayer emulsions

This research aimed to induce repulsive gelation in Citrem-stabilized O/W emulsions by creating a secondary layer of chitosan around the droplets. A range of chitosan concentration (0-0.25wt%) and degree of...

Palmarosa essential oil encapsulated in chitosan nanoparticles by ionotropic gelation: formulation and characterization

In this study, chitosan nanoparticles containing palmarosa essential oil (PEO-CNPs) were formed by ionotropic gelation, consisting of two parts: emulsion preparation followed by ionotropic gelation encapsulation with tripolyphosphate ions (TPP) as a crosslinker. The encapsulation method was optimized by varying three parameters, including chitosan concentration, initial oil loading in the emulsion and TPP concentration. The effects of these parameters on the encapsulation efficiency (EE) and loading capacity (LC) were analyzed. EE had an initial increase followed by a decrease in the range of three parameters. However, LC rose with varying initial oil content while it reduced with changing polymer and TPP concentration. The optimum experiment with the highest EE (10.0 g/L of chitosan, 5.0 g/L of TPP and 30.0 g/L PEO) was chosen to analyze the particle size using Dynamic Light Scanning method (DLS). With DLS measurement, the z-average diameter was 235.3 nm, and the particle size distribution was in the range of 100 – 500 nm.

The effect of chitosan concentration on flocculation efficiency microalgae Porphyridium cruentum (Rhodhophyta)

Porphyridium cruentum is a species of red microalgae belongs to the family Porphyridiophyceae, divisi Rhodophyta. P. cruentum contains a lot of nutrients which are very useful as functional food. The purpose of this research is to determine the effect of chitosan concentration on the flocculation efficiency of P. cruentum. In this study, there were two treatments, namely the concentration of chitosan and the time of flocculation with 3 replications. Chitosan used were 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 ppm. The flocculation efficiency were carried out at 10, 20, 30 and 40 minutes. The highest density was reached on the seventh day as amount 1720x104cell/mL. The results showed that there was an effect of Chitosan concentration on flocculation efficiency (Anova two way; Fcount = 4.109; df (9; 80); p=0.01). In addition, there was an effect of flocculation time on flocculation efficiency (Anova two way, Fcount = 4.498; df (3;80)). Furthermore, there was an interaction effect between the chitosan concentration and the time of flocculation. (Anova two way; Fcount = 26.635; df (2;80)). The greater the value of chitosan concentration given the greater the value of flocculation efficiency.

IMMOBILIZATION OF CELLULASES ON CHITOSAN: APPLICATION FOR SUGARCANE BAGASSE HYDROLYSIS

In this work, a commercial cellulolytic cocktail was immobilized on glutaraldehyde activated chitosan gel. The chitosan concentration in the gel preparation, pH, immobilization time and enzymatic loading were evaluated. Immobilized cellulases showed better hydrolysis performance when an enzyme loading of 134 mg protein/g carrier was used for immobilization at pH 9.0 for 30 minutes. Hydrolysates with a glucose content of 13.43 and 10.35 g/L were obtained when Avicel and pretreated sugarcane bagasse were used as substrate, respectively. Immobilized cellulase lost 60% of its hydrolysis performance after 8 cycles using Avicel, and 75% after 6 cycles for sugarcane bagasse. The hydrolysis performance associated with the reuse of the immobilized cellulases indicates that an improvement in the immobilization of cellulases, coupled with an improvement in the pretreatment of lignocellulosic biomass, will allow the development of a continuous hydrolysis system with the enzyme retained in the reactor.

ANALISIS MUTU IKAN PATIN (Pangasius sp.) SALAI DENGAN PEMBERIAN KITOSAN DAN LAMA PENGASAPAN SEBAGAI RANCANGAN LKPD BIOLOGI SMA

The aims to determine the effect of chitosan concentration and smoking time on the quality of smoked catfish (Pangasius sp.) and produce Student Worksheet design. This research was divided into two step, there is experimental step and the Student Worksheet design. At the experimental step, used a factorial Completely Randomized Design (CRD). Factor I is the concentration of chitosan, and factor II is duration of smoking time. This study consisted of 12 treatments with 3 replications so that there were 36 experimental units. Parameters observed were protein content, fat content, water content and organoleptic on appearance, scent, taste and texture. The results showed that the effect of chitosan concentration and smoking time had an effect on treated with 3% chitosan and 3 days of smoking showed the best results, with the total protein content was 35.89%, fat content was 29.72% and water content was 15.27%. Meanwhile, the organoleptic test results of smoked catfish on the aspects of appearance, scent, taste and texture, the best treatment was also found in smoked catfish with 3% chitosan treatment and 3 days of smoking time. The results can be used as a student worksheet design on Food Additives material for class XI high school.

Ability of chitosan membrane from blood clam (Anadara granosa) shells to removal iron level (Fe) in water

Clam shells are a food solid waste that increases and accumulates every day, causing environmental pollution. Clam shells (Anadora granosa) contain chitin. Chitin can be processed into chitosan, which effective in absorbing heavy metals. To increase absorption, chitosan modified into a membrane. The purpose of this study was to analyze the membrane's ability of chitosan waste from blood clam shells to reduce levels of iron in water. The design of this study was pure experimentation. The object of research used was iron-containing artificial water made from iron salt powder at a dose of 4.50 mg/L. Variations of Chitosan concentration to the membranes manufacture were 1%, 2%, 3%. Analysis of the data used was One Way Anova Test. Chitosan characterization analysis using XR Diffraction showed that chitosan was isolated from shells at peak angles at 17.99o and 34.04o. The highest percentage of iron reduction was given by membranes with a chitosan concentration of 3%, which have decreased of 94%. The results showed that the membrane of blood shells chitosan can reduce iron levels in water and can be an alternative material in the utilization of clamshell waste. The membrane can be applied in simple water treatment filtration systems in the community.

Optimization of Chitosan Concentration on The Quality and Shelf Life of Frozen Rohu (Labeo rohita) Fillets

A natural product such as chitosan promotes health benefits and extends the shelf life of the processed products. Chitosan has been used as a food additive due to its antioxidant and antimicrobial activities. This study was conducted to optimize the chitosan concentration as a coating agent on the quality and shelf life of Labeo rohita fillets during frozen storage. Chitosan (CH) solutions were prepared with 0.5 %, 1 %, and 2 % (w/v) CH in 1 % (v/v) glacial acetic acid for coating application. The control (0% CH) and CH treated fish fillets were stored at -18 °C for 14 weeks and assessed for chemical (pH, thiobarbituric acid reactive substances (TBARS), total volatile basic nitrogen (TVB-N), and K-value), microbiological (aerobic plate count (APC)), and sensory properties. The pH, TBARS, and K-value of 1 % and 2 % CH treated fish fillets were acceptable up to 14th week of storage, while TVB-N value was permissible up to 12th week of storage. After 14th week of storage, the APC of control, 0.5 %, 1 %, and 2 % CH treated fillets were 7.18 log CFU/g, 5.02 log CFU/g, 4.13 log CFU/g, and 3.21 log CFU/g, respectively. The fish fillets treated with 1 % and 2 % CH had acceptable sensory attributes up to 12th week, while control fillets were unacceptable after the 6th week of storage. This study demonstrated that 1% CH could be used as a natural additive for keeping the quality and extending the shelf life of L. rohita fillets during frozen storage.

The effect of plasticizers and chitosan concentration on the structure and properties of Gracilaria sp.-based thin films for food packaging purpose

Gracilaria sp. is well known as one kind of species of red algae. The major component of polysaccharide in this alga is agar that mostly used for making thin film. In this study, the Gracilaria sp.-based thin film had been prepared using two plasticizers (glycerol and sorbitol, 0.1, 0.2, and 0.3 wt %), and chitosan (1, 2, and 3 wt %). The FT-IR analysis confirmed the interaction that happened among the component of the mixture of Gracilaria sp., plasticizers, and chitosan was based on hydrogen bonding due to the presence of -OH and -NH2 groups. The plasticizers and chitosan concentration have significant role to the mechanical properties of Gracilaria sp.-based thin film. The optimum concentration of plasticizers and chitosan based on mechanical testing result was found at 0.2 and 3.0 wt %, respectively. At those concentrations, the thin film that prepared with sorbitol showed the highest mechanical properties. Other characterizations, i.e. TGA (Thermogravimetric Analysis), SEM (Scanning Electron Microscopy), and WVP (Water Vapor Permeability) also brought the same result. The antimicrobial properties of the as prepared thin film in the presence of chitosan on agar medium and as a packaging on selected bread showed the Gracilaria sp.-based thin films was able to inhibit the growth of microbes. This antimicrobial activity can be used to declare the potential of Gracilaria sp.-based thin film as a new active food packaging.

Pemanfaatan Kitosan untuk Menurunkan Kadar Logam Pb dalam Perairan yang Tercemar Minyak Bumi

Kitosan telah banyak dimanfaatkan dalam berbagai bidang kehidupan manusia, salah satunya dijadikan sebagai adsorben logam berat. Logam berat timbal (Pb) merupakan polutan yang mencemari perairan dan bersifat toksik. Penelitian ini bertujuan untuk menguji kapasitas dan daya adsorpsi larutan kitosan komersial dan non komersial (produk sendiri) dalam konsentrasi berbeda terhadap logam Pb. Metode penelitian yang digunakan yaitu eksperimental laboratoris dengan rancangan percobaan Faktorial 2 Aras dengan menggunakan 1 kontrol (0 %) dan 4 perlakuan yaitu konsentrasi 0,5% (A), 1% (B), 1,5% (C) dan 2% (D). Hasil penelitian menunjukkan bahwa terdapat interaksi antara sumber kitosan dengan konsentrasi kitosan untuk menurunkan kandungan Pb. Dosis terbaik yang mampu menurunkan logam Pb untuk kitosan komersial yaitu pada konsentrasi 1% dengan kapasitas penyerapan sebesar 0,228 mg/g dan kemampuan penyerapan sebesar 87,870 %. Sedangkan kitosan non komersial mampu menurunkan logam Pb pada konsentrasi 1,5% dengan kapasitas penyerapan sebesar 0,143 mg/g dan kemampuan penyerapan sebesar 82,660 %. Chitosan has been widely used in various fields of human life, one of which is used as a heavy metal adsorbent. Lead heavy metals (Pb) is a pollutants that pollutes the waters and is toxic. This study aims to examine the capacity and adsorption capacity of commercial and non-commercial chitosan solutions (own products) in different concentrations of Pb metal. The research method used is an experimental laboratories with chitosan from the isolation itself and commercial chitosan. The research method used is an experimental laboratory with 2 Aras factorial experimental design using 1 control (0%) and 4 treatments namely a concentrations of 0.5% (A), 1% (B), 1.5% (C) and 2% (D). The results showed that there was an interaction between chitosan sources and chitosan concentration to reduce Pb content. The best dose that can reduce Pb metal for commercial chitosan is at a concentration of 1% with an adsorption capacity of 0.228 mg/g, and an adsorption ability of 87.870 %. while non-commercial chitosan can reduce Pb metal at a concentration of 1.5 % with an adsorption capacity of 0.143 mg/g and an adsorption ability of 82.660 %.