Re: The Effects of Male Age on Sperm Analysis by Motile Sperm Organelle Morphology Examination (MSOME)

Sixty semen ejaculates from 10 mature bulls, 5 each of Jafarabadi and Mehsana breed, were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The mean values of motile and progressively motile spermatozoa observed in fresh semen of Jafarabadi and Mehsana bulls (79.77±1.62 and 61.80±1.85, and 78.90±1.22 and 61.37±1.58%) were highly significantly (P<0.01) reduced (51.20±1.57 and 33.20±1.45, and 52.10±1.70 and 34.30±1.54 %, respectively) in post-thawed semen. The average path velocity, straight line velocity and curvilinear velocity (μm/sec) of spermatozoa of Jafarabadi and Mehsana bulls noted in fresh semen were also reduced highly significantly (P<0.01) in frozen-thawed semen. Among the other velocity parameters, amplitude of lateral head displacement (μm), elongation (%) and medium motile sperm (%) increased, while beat-cross frequency (Hz), straightness (%), linearity (%), sperm area (μm<sup>2</sup>) and rapidly motile sperm (%) decreased significantly in post-thawed sperms when compared with the fresh sperm of both Jafarabadi and Mehsana bulls. The initial motility and live sperm per cent were significantly correlated with CASA traits of fresh and frozen-thawed semen, and all the sperm motility and velocity traits of fresh and frozen-thawed semen assessed by CASA were significantly interrelated among both the breeds. The interrelationships were stronger in Mehsana bulls as compared to Jafarabadi bulls.

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Relationship between conventional sperm parameters and motile sperm organelle morphology examination (MSOME)

International Journal of Andrology ◽

10.1111/j.1365-2605.2012.01249.x ◽

2012 ◽

Vol 35 (4) ◽

pp. 491-498 ◽

Cited By ~ 36

Author(s):

A. Perdrix ◽

R. Saïdi ◽

J. F. Ménard ◽

E. Gruel ◽

J. P. Milazzo ◽

...

Keyword(s):

Sperm Parameters ◽

Motile Sperm ◽

Organelle Morphology

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Design, Fabrication, and Testing of a Microfluidic Device for Thermotaxis and Chemotaxis Assays of Sperm

SLAS TECHNOLOGY Translating Life Sciences Innovation ◽

10.1177/2472630318783948 ◽

2018 ◽

Vol 23 (6) ◽

pp. 507-515 ◽

Cited By ~ 2

Author(s):

Yong-Jun Ko ◽

Joon-Ho Maeng ◽

Seung Yong Hwang ◽

Yoomin Ahn

Keyword(s):

Microfluidic Device ◽

Low Cost ◽

Motile Sperm ◽

Mouse Sperm ◽

Sperm Analysis ◽

Mammalian Sperm ◽

Fundamental Understanding ◽

Sperm Migration ◽

First Time ◽

Sperm Motion

Infertile couples needing assisted reproduction are increasing, so a fundamental understanding of motile sperm migration is required. This paper presents an advanced microfluidic device for sperm motion analysis utilizing chemotaxis and thermotaxis simultaneously for the first time. The proposed device is a transparent polydimethylsiloxane- and glass-based microfluidic chip system providing a low-cost, useful, and disposable platform for sperm analysis. The concentration gradient of the chemoattractant (acetylcholine) and the temperature difference are formed along the microchannel. The temperature gradient is generated and controlled by a microheater and microsensor. Thermotactic and chemotactic responses of mouse sperm were examined using the proposed device. Experimental results show that motile mouse sperm are attracted more sensitively under integrated conditions of chemotaxis and thermotaxis rather than individual conditions of chemotaxis and thermotaxis. This sperm analysis device is expected to be a useful tool for the study of mammalian sperm migration and the improvement of artificial insemination techniques.

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Post-testicular sperm maturation and identification of an epididymal protein in the Japanese quail (Coturnix coturnix japonica)

Reproduction ◽

10.1530/rep-13-0566 ◽

2014 ◽

Vol 147 (3) ◽

pp. 265-277 ◽

Cited By ~ 18

Author(s):

Brett Nixon ◽

Katherine A Ewen ◽

Klara M Krivanek ◽

John Clulow ◽

Grahame Kidd ◽

...

Keyword(s):

Computer Assisted ◽

Testicular Development ◽

Major Protein ◽

Motile Sperm ◽

Testicular Sperm ◽

Sperm Analysis ◽

Testicular Spermatozoa ◽

Mammalian Sperm ◽

Epididymal Maturation ◽

Sperm Development

The role of the avian epididymis in post-testicular development and capacitation was examined to assess whether avian spermatozoa undergo any processes similar to those characteristic of mammalian sperm development. We found no evidence of a need for quail sperm to undergo capacitation and 90% of testicular sperm could bind to a perivitelline membrane and acrosome react. However, computer-assisted sperm analysis showed that 20% of testicular sperm from the quail were capable of movement and only about 12% of the motile sperm would have a curvilinear velocity greater than the mean for sperm from the distal epididymis. Nevertheless, epididymal transit was associated with increases in mean sperm velocity and the proportion of motile sperm. Together, these findings explain why earlier workers have achieved some fertilizations following inseminations of testicular spermatozoa and also demonstrate the need for some epididymal maturation of avian spermatozoa. Analysis of the electrophoretic profile of quail epididymal luminal proteins revealed that only one major protein (∼16 kDa) is secreted by the epididymis and it was virtually the only protein secreted by the ipsilateral epididymis following unilateral orchidectomy. Mass spectrometry showed that this protein is hemoglobin; this finding was confirmed using anti-hemoglobin antibodies. It is suggested that hemoglobin may support sperm metabolism in the quail epididymis, aid in motility, and/or serve as an antioxidant.

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