In vitro evaluation of Zeolite-nHA blended PCL/PLA nanofibers for dental tissue engineering

Dentin tissue engineering is one of innovative and effective regenerative method for treatment of dental pulp and dental tissue, and our research concentrated on preparing an effective scaffold substrate containing carbon Nano tube in polycaprolactone Nano fiber matrix. Our characterization showed benefits of this complex structure and its effect on odontoblast cell interaction. The results indicated that the prepared nanofiber polymer contains 4% CNT have a great potential as biocompatible materials for use in dental tissue engineering and odontoblast cell interaction.

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Study on Culture of Human Dental Pulp Stem Cells to Apply in Tissue Engineering

Journal of Biomimetics Biomaterials and Tissue Engineering ◽

10.4028/www.scientific.net/jbbte.11.13 ◽

2011 ◽

Vol 11 ◽

pp. 13-20 ◽

Cited By ~ 5

Author(s):

Tran Le Bao Ha ◽

Doan Nguyen Vu ◽

To Minh Quan ◽

Ngoc Phan Kim ◽

Hung Hoang Tu ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Dental Tissue ◽

Dentin Surface ◽

Human Dental Pulp ◽

Dental Tissue Engineering ◽

High Level

Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.

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In vitro evaluation of novel Zeolite-hydroxyapatite blended scaffold for dental tissue engineering

10.21203/rs.2.20452/v1 ◽

2020 ◽

Cited By ~ 1

Author(s):

Sanam Mohandesnezhad ◽

Effat Alizadeh ◽

Yunes Pilehvar-Soltanahmadi ◽

Soodabeh Davaran ◽

Arash Goodarzi ◽

...

Keyword(s):

Tissue Engineering ◽

Dental Pulp ◽

Pulp Tissue ◽

Dental Tissue ◽

Electrospun Scaffolds ◽

Th Cell ◽

Dental Pulp Tissue ◽

Dental Tissue Engineering ◽

Poly Caprolactone

Abstract Main purpose of tissue engineering is creating appropriate conditions for the regeneration of tissues. Dental pulp-derived stem cells due to differentiation capacity and angiogenic properties have potential to regenerate dental pulp tissue. In the current experimental study poly caprolactone and poly L-lactic acid were synthesized by ring-opening polymerization method. The nano-hydroxyapatite and Zeolite were obtained by hydrothermal method. Morphological features and crystals properties of nHA and Zeolite were studied by X-ray diffraction. Nanofibers were fabricated using electrospinning method and investigated by FT-IR spectroscopy. DPSCs obtained from human source and proliferation and viability of them on electrospun scaffolds were evaluated by MTT assay. Also, the adhesion and proliferation of hDPSCs were investigated by SEM. The results showed that hDPSCs have the most viability and proliferation on the 1 st , 7 th , 14 th days on PCL-PLA/Zeolite scaffolds and maximum on the 3 rd day on PCL-PLA/nHA scaffolds. On the days of 7 th and 14 th , cell growth on scaffolds containing both nHA and Zeolite is better than sample that nHA is used alone with PCL-PLA. Briefly, by these results can be understand that Zeolite is a good agent in bone and tooth tissue engineering applications. More studies requires to investigate Zeolite effect on scaffold properties.

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