scholarly journals In vitro evaluation of novel Zeolite-hydroxyapatite blended scaffold for dental tissue engineering

2020 ◽  
Author(s):  
Sanam Mohandesnezhad ◽  
Effat Alizadeh ◽  
Yunes Pilehvar-Soltanahmadi ◽  
Soodabeh Davaran ◽  
Arash Goodarzi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Dental Tissue ◽  
Electrospun Scaffolds ◽  
Th Cell ◽  
Dental Pulp Tissue ◽  
Dental Tissue Engineering ◽  
Poly Caprolactone

Abstract Main purpose of tissue engineering is creating appropriate conditions for the regeneration of tissues. Dental pulp-derived stem cells due to differentiation capacity and angiogenic properties have potential to regenerate dental pulp tissue. In the current experimental study poly caprolactone and poly L-lactic acid were synthesized by ring-opening polymerization method. The nano-hydroxyapatite and Zeolite were obtained by hydrothermal method. Morphological features and crystals properties of nHA and Zeolite were studied by X-ray diffraction. Nanofibers were fabricated using electrospinning method and investigated by FT-IR spectroscopy. DPSCs obtained from human source and proliferation and viability of them on electrospun scaffolds were evaluated by MTT assay. Also, the adhesion and proliferation of hDPSCs were investigated by SEM. The results showed that hDPSCs have the most viability and proliferation on the 1 st , 7 th , 14 th days on PCL-PLA/Zeolite scaffolds and maximum on the 3 rd day on PCL-PLA/nHA scaffolds. On the days of 7 th and 14 th , cell growth on scaffolds containing both nHA and Zeolite is better than sample that nHA is used alone with PCL-PLA. Briefly, by these results can be understand that Zeolite is a good agent in bone and tooth tissue engineering applications. More studies requires to investigate Zeolite effect on scaffold properties.

Study on Culture of Human Dental Pulp Stem Cells to Apply in Tissue Engineering

2011 ◽  
Vol 11 ◽  
pp. 13-20 ◽  
Author(s):  
Tran Le Bao Ha ◽  
Doan Nguyen Vu ◽  
To Minh Quan ◽  
Ngoc Phan Kim ◽  
Hung Hoang Tu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Dental Tissue ◽  
Dentin Surface ◽  
Human Dental Pulp ◽  
Dental Tissue Engineering ◽  
High Level

Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.

Dental Pulp Tissue Engineering Using Mesenchymal Stem Cells: a Review with a Protocol

2018 ◽  
Vol 14 (5) ◽  
pp. 668-676 ◽  
Author(s):  
Tomoatsu Kaneko ◽  
Bin Gu ◽  
Phyo Pyai Sone ◽  
Su Yee Myo Zaw ◽  
Hiroki Murano ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Dental Pulp Tissue

Dental pulp tissue engineering of pulpotomized rat molars with bone marrow mesenchymal stem cells

Odontology ◽  
2016 ◽  
Vol 105 (4) ◽  
pp. 392-397 ◽  
Author(s):  
Takafumi Ito ◽  
Tomoatsu Kaneko ◽  
Yukiko Sueyama ◽  
Reika Kaneko ◽  
Takashi Okiji
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Marrow Mesenchymal Stem Cells ◽  
Dental Pulp Tissue ◽  
Rat Molars

Suitability of Different Natural and Synthetic Biomaterials for Dental Pulp Tissue Engineering

2018 ◽  
Vol 24 (3-4) ◽  
pp. 234-244 ◽  
Author(s):  
Kerstin M. Galler ◽  
Ferdinand P. Brandl ◽  
Susanne Kirchhof ◽  
Matthias Widbiller ◽  
Andreas Eidt ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Natural And Synthetic

scholarly journals Cell-free Approaches for Dental Pulp Tissue Engineering

2014 ◽  
Vol 40 (4) ◽  
pp. S41-S45 ◽  
Author(s):  
Kerstin M. Galler ◽  
Andreas Eidt ◽  
Gottfried Schmalz
Keyword(s):  
Tissue Engineering ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Dental Pulp Tissue

scholarly journals Dental pulp tissue engineering

2011 ◽  
Vol 22 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Flávio Fernando Demarco ◽  
Marcus Cristian Muniz Conde ◽  
Bruno Neves Cavalcanti ◽  
Luciano Casagrande ◽  
Vivien Thiemy Sakai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Dental Pulp ◽  
Regeneration Capacity ◽  
Pulp Tissue ◽  
Regenerative Endodontics ◽  
Mesenchymal Tissue ◽  
Dental Pulp Tissue ◽  
Anatomical Arrangement ◽  
Exciting Time ◽  
The Ideal

Dental pulp is a highly specialized mesenchymal tissue that has a limited regeneration capacity due to anatomical arrangement and post-mitotic nature of odontoblastic cells. Entire pulp amputation followed by pulp space disinfection and filling with an artificial material cause loss of a significant amount of dentin leaving as life-lasting sequelae a non-vital and weakened tooth. However, regenerative endodontics is an emerging field of modern tissue engineering that has demonstrated promising results using stem cells associated with scaffolds and responsive molecules. Thereby, this article reviews the most recent endeavors to regenerate pulp tissue based on tissue engineering principles and provides insightful information to readers about the different aspects involved in tissue engineering. Here, we speculate that the search for the ideal combination of cells, scaffolds, and morphogenic factors for dental pulp tissue engineering may be extended over future years and result in significant advances in other areas of dental and craniofacial research. The findings collected in this literature review show that we are now at a stage in which engineering a complex tissue, such as the dental pulp, is no longer an unachievable goal and the next decade will certainly be an exciting time for dental and craniofacial research.

scholarly journals Dental Pulp Regeneration: Insights from Biological Processes

2017 ◽  
Vol 20 (1) ◽  
pp. 10-16
Author(s):  
Cristina Retana-Lobo DDS, MSD
Keyword(s):  
Tissue Engineering ◽  
Dental Pulp ◽  
Short Review ◽  
Biological Processes ◽  
Pulp Tissue ◽  
Pulp Regeneration ◽  
Dental Tissues ◽  
Current State ◽  
Signaling Mechanisms ◽  
Dental Pulp Tissue

One of the major approaches on dentistry research in this century is the development of biological strategies (tissue engineering) to regenerate/ biomineralize lost dental tissues. During dentin-pulp regeneration, the interaction between stem cells, signaling molecules, biomaterials and the microenvironment in the periapical area drives the process for dental pulp tissue engineering.  Understanding the signaling mechanisms and interactions involved with the biological process for the formation of a new tissue, is essential. The knowledge of the micro-environment is the key for the application of tissue engineering.  The present article is a short review of the current state of this topic, with the purpose of showing insights of pulp regeneration.

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