The flavonoid quercetin reduces cell migration and increases NIS and E-cadherin mRNA in the human thyroid cancer cell line BCPAP

Author(s):  
Carlos Frederico Lima Gonçalves ◽  
Fabio Hecht ◽  
Juliana Cazarin ◽  
Rodrigo Soares Fortunato ◽  
Mario Vaisman ◽  
...  
2009 ◽  
Vol 50 (8) ◽  
pp. 1364-1370 ◽  
Author(s):  
O. Prante ◽  
S. Maschauer ◽  
V. Fremont ◽  
J. Reinfelder ◽  
R. Stoehr ◽  
...  

2010 ◽  
Vol 110 (6) ◽  
pp. 1495-1503 ◽  
Author(s):  
Meng-Ching Chen ◽  
Ching-Han Yu ◽  
Shyi-Wu Wang ◽  
Hsiao-Fung Pu ◽  
Shu-Fen Kan ◽  
...  

2002 ◽  
Vol 175 (2) ◽  
pp. 459-465 ◽  
Author(s):  
ST Chen ◽  
JD Lin ◽  
KH Lin

The expression of TSH receptor (TSHR) gene is frequently lost in thyroid cancers during the process of dedifferentiation that involves perturbation of several nuclear transcription factors. We have established that thyroid hormone receptor beta1 (TRbeta1) is associated with the loss of TSHR gene expression in an anaplastic human thyroid cancer cell line, ARO. To demonstrate that TRbeta1 regulates TSHR gene expression, we performed electrophoresis mobility shift and 3,5,3'-triiodothyronine (T3) transactivation assays. As expected, TRbeta1 bound the synthesized oligomer containing TSHR promoter sequence by heterodimerizing with retinoid X receptor. When a chimeric reporter pTRCAT5'-146 enclosing the minimal TSHR promoter was applied for T3 transactivation assay, two TRbeta1-overexpressing transfectants of ARO cells (ARO1 and ARO2) demonstrated higher basal activity than their parental cells. Consequentially, T3 suppressed the reporter gene activity only in ARO1 and ARO2, but not in ARO cells. A point mutation creating a cAMP response element (CRE) in the reporter pTRCAT5'-146 CRE led to T3-induced suppression of the reporter gene in ARO cells without changing the basal or T3-induced activities in ARO1 and ARO2 cells. We conclude that the regulatory effect of T3 on TSHR gene expression is TR- and promoter DNA sequence-determined.


1992 ◽  
Vol 28 (11) ◽  
pp. 1904-1909 ◽  
Author(s):  
Noritaka Onoda ◽  
Eiji Ohmura ◽  
Toshio Tsushima ◽  
Yoshito Ohba ◽  
Naoya Emoto ◽  
...  

2021 ◽  
Vol 123 (3) ◽  
pp. 151700
Author(s):  
Marjan Ghorbani-Anarkooli ◽  
Sara Dabirian ◽  
Adib Zendedel ◽  
Hasan Moladoust ◽  
Mohammad hadi Bahadori

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