Real-time PCR detection and quantification of vector trichodorid nematodes and Tobacco rattle virus

2006 ◽  
Vol 20 (3-4) ◽  
pp. 203-211 ◽  
Author(s):  
R. Holeva ◽  
M.S. Phillips ◽  
R. Neilson ◽  
D.J.F. Brown ◽  
V. Young ◽  
...  
2021 ◽  
Vol 9 (4) ◽  
pp. 800
Author(s):  
Francesca Servadei ◽  
Silvestro Mauriello ◽  
Manuel Scimeca ◽  
Bartolo Caggiano ◽  
Marco Ciotti ◽  
...  

The aim of this study was to investigate the persistence of SARS-CoV-2 in post-mortem swabs of subjects who died from SARS-CoV-2 infection. The presence of the virus was evaluated post-mortem from airways of 27 SARS-CoV-2 positive patients at three different time points (T1 2 h; T2 12 h; T3 24 h) by real-time PCR. Detection of antibodies to SARS-CoV-2 was performed by Maglumi 2019-nCoV IgM/IgG chemiluminescence assay. SARS-CoV-2 viral RNA was still detectable in 70.3% of cases within 2 h after death and in 66,6% of cases up to 24 h after death. Our data showed an increase of the viral load in 78,6% of positive individuals 24 h post-mortem (T3) in comparison to that evaluated 2 h after death (T1). Noteworthy, we detected a positive T3 post-mortem swab (24 h after death) from 4 subjects who were negative at T1 (2 h after death). The results of our study may have an important value in the management of deceased subjects not only with a suspected or confirmed diagnosis of SARS-CoV-2, but also for unspecified causes and in the absence of clinical documentation or medical assistance.


2007 ◽  
Vol 8 (6) ◽  
pp. 803-809 ◽  
Author(s):  
CECILE FRANÇOIS ◽  
CHANTAL CASTAGNONE ◽  
NEIL BOONHAM ◽  
JENNY TOMLINSON ◽  
REBECCA LAWSON ◽  
...  

Apmis ◽  
2009 ◽  
Vol 117 (11) ◽  
pp. 856-860 ◽  
Author(s):  
SARA THULIN HEDBERG ◽  
PER OLCÉN ◽  
HANS FREDLUND ◽  
PAULA MÖLLING

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