Intercontinental spread of Asian-origin H7 avian influenza viruses by captive bird trade in 1990's

Abstract Background H7N9 avian influenza virus (AIV) including highly and low pathogenic viruses have been detected in China since 2013. H7N9 AIV has a high mortality rate after infection in humans, and most human cases have close contacted with poultry in the live poultry market. Therefore, it is necessary to develop a rapid point-of-care testing (POCT) technique for H7N9 AIV detection. Methods The H7N9 AIV was inactivated and purified, and was used as the antigen to immunize BALB/c. Twelve H7-HA specific monoclonal antibodies (McAbs) were produced through the hybridoma technique. The McAb 10A8 was conjugated with colloid gold as detecting antibody; McAb 9B6 was dispensed on the nitrocellulose membran as the capture test line and the Goat-anti mouse IgG antibody was dispensed as control line respectively. The immunochromatographic strip was prepared. Results The analysis of ELISA and virus neutralization test showed that the obtained McAbs specifically recognized H7 HA. Based on the prepared strip, the detection of H7 AIV was achieved within 10 min. No cross-reaction occurred between H7 AIVs and other tested viruses. The detection limit of the strip for H7 was 2.4 log10EID50/0.1 mL for chicken swab samples. Conclusion The McAbs were specific for H7 and the immunochromatographic strip developed in this study was convenient, rapid and reliable for the detection of H7 AIV. The strip could provide an effective method for the rapid and early detection of H7 AIV.

Download Full-text

A proof-of-principle study to identify suitable vaccine seed candidates to combat introductions of Eurasian lineage H5 and H7 subtype avian influenza viruses

Avian Pathology ◽

10.1080/03079457.2010.513376 ◽

2010 ◽

Vol 39 (5) ◽

pp. 375-382 ◽

Cited By ~ 7

Author(s):

Maria Serena Beato ◽

Isabella Monne ◽

Marzia Mancin ◽

Elena Bertoli ◽

Ilaria Capua

Keyword(s):

Avian Influenza ◽

Influenza Viruses ◽

Avian Influenza Viruses ◽

Eurasian Lineage ◽

H7 Subtype ◽

Proof Of Principle

Download Full-text

Heterologous Humoral Response against H5N1, H7N3, and H9N2 Avian Influenza Viruses after Seasonal Vaccination in a European Elderly Population

Vaccines ◽

10.3390/vaccines5030017 ◽

2017 ◽

Vol 5 (3) ◽

pp. 17 ◽

Cited By ~ 2

Author(s):

Ivan Sanz ◽

Silvia Rojo ◽

Sonia Tamames ◽

José Eiros ◽

Raúl Ortiz de Lejarazu

Keyword(s):

Avian Influenza ◽

Elderly Population ◽

Humoral Response ◽

Influenza Viruses ◽

Avian Influenza Viruses

Download Full-text

Antibody responses to avian influenza viruses in wild birds broaden with age

Proceedings of The Royal Society B Biological Sciences ◽

10.1098/rspb.2016.2159 ◽

2016 ◽

Vol 283 (1845) ◽

pp. 20162159 ◽

Cited By ~ 11

Author(s):

Sarah C. Hill ◽

Ruth J. Manvell ◽

Bodo Schulenburg ◽

Wendy Shell ◽

Paul S. Wikramaratna ◽

...

Keyword(s):

Avian Influenza ◽

Haemagglutination Inhibition ◽

Wild Birds ◽

Influenza Viruses ◽

Antibody Responses ◽

Immune Recognition ◽

Avian Influenza Viruses ◽

Highly Pathogenic ◽

Age Related ◽

Pathogenic H5n1

For viruses such as avian influenza, immunity within a host population can drive the emergence of new strains by selecting for viruses with novel antigens that avoid immune recognition. The accumulation of acquired immunity with age is hypothesized to affect how influenza viruses emerge and spread in species of different lifespans. Despite its importance for understanding the behaviour of avian influenza viruses, little is known about age-related accumulation of immunity in the virus's primary reservoir, wild birds. To address this, we studied the age structure of immune responses to avian influenza virus in a wild swan population ( Cygnus olor ), before and after the population experienced an outbreak of highly pathogenic H5N1 avian influenza in 2008. We performed haemagglutination inhibition assays on sampled sera for five avian influenza strains and show that breadth of response accumulates with age. The observed age-related distribution of antibody responses to avian influenza strains may explain the age-dependent mortality observed during the highly pathogenic H5N1 outbreak. Age structures and species lifespan are probably important determinants of viral epidemiology and virulence in birds.

Download Full-text