Disrupting effects of lithium chloride in the rat ovary: Involves impaired formation and function of corpus luteum

The distribution of gelatinases/matrix metalloproteinases-2 and -9 (MMP-2 and MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in neonatal and gonadotropin-primed immature rat ovaries was studied by immunofluorescent microscopy. Immature female Long-Evans rats were primed with 15 IU pregnant mare's serum gonadotropin (PMSG) in 100 microliters PBS. Two days later, to induce ovulation, the rats were injected with human chorionic gonadotropin (hCG, 5 IU/100 microliters PBS). The animals were killed at appropriate times and the ovaries removed and processed for cryostat or paraffin sectioning. Ovaries were also obtained from 7-day-old neonatal rats and processed as above. In the neonatal rat ovary, MMP-2 was present in the follicle and in the ovarian surface epithelium. MMP-9 was not detectable in the neonatal ovary. TIMP-1 was present in the oocyte and in the surface epithelium. In the PMSG-primed ovary, MMP-2 was present in the granulosa and thecal cells of the ovary. MMP-9 distribution, however, was restricted to the interstitial and thecal cells. TIMP-1 was mainly present in the blood vessels and thecal cells, with minor staining in the granulosa cells. In the developing corpus luteum, luteal and endothelial cells were positive for MMP-2. MMP-9 localization was restricted to the plasma membrane of the luteal and interstitial cells. TIMP-1 was clearly observed in the luteal capillaries and, to a lesser extent, in the luteal cell plasma membrane. This distribution of MMP-2, MMP-9, and TIMP-1 in the corpus luteum persisted throughout the life span of the corpus luteum. The spatial and temporal distribution of the gelatinases and TIMP-1 suggests unique roles for these proteins in the rat ovary.

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Spatial distribution of progesterone in bovine uterus in relation to corpus luteum location and function

Theriogenology ◽

10.1016/0093-691x(94)90831-3 ◽

1994 ◽

Vol 41 (8) ◽

pp. 1663-1671 ◽

Cited By ~ 6

Author(s):

W.A. Cerbito ◽

F.V. Quero ◽

C.R. Balagapo ◽

K. Miyazawa ◽

K. Sato

Keyword(s):

Spatial Distribution ◽

Corpus Luteum ◽

And Function

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THE GROWTH AND FUNCTION OF THE CORPUS LUTEUM

Physiological Reviews ◽

10.1152/physrev.1928.8.3.313 ◽

1928 ◽

Vol 8 (3) ◽

pp. 313-345 ◽

Cited By ~ 24

Author(s):

S. A. Asdell

Keyword(s):

Corpus Luteum ◽

And Function

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Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

Reproduction ◽

10.1530/rep-15-0085 ◽

2015 ◽

Vol 150 (3) ◽

pp. 217-225 ◽

Cited By ~ 35

Author(s):

Koumei Shirasuna ◽

Haruka Matsumoto ◽

Shuichi Matsuyama ◽

Koji Kimura ◽

Heinrich Bollwein ◽

...

Keyword(s):

Corpus Luteum ◽

Neutrophil Migration ◽

Peripheral Circulation ◽

Interferon Tau ◽

Bovine Corpus Luteum ◽

Activated Neutrophils ◽

Endocrine Factor ◽

Uterine Environment ◽

And Function

When pregnancy is established, interferon tau (IFNT), a well-known pregnancy recognition signal in ruminants, is secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT acts as an endocrine factor on the corpus luteum (CL) to induce refractory ability against the luteolytic action of PGF2α. Hypothesising that IFNT may influence not only the uterine environment but also the CL in cows via local or peripheral circulation, we investigated qualitative changes in the CL of pregnant cows during the maternal recognition period (day 16) and the CL of non-pregnant cows. The CL of pregnant animals had a higher number of neutrophils, and the expression of interleukin 8 (IL8) mRNA and its protein was higher as well as compared with the CL of non-pregnant animals. Although IFNT did not affect progesterone (P4) secretion and neutrophil migration directly, it stimulated IL8 mRNA expression on luteal cells (LCs), influencing the neutrophils, resulting in the increased migration of IFNT-activated neutrophils. Moreover, both IFNT-activated neutrophils and IL8 increased P4 secretion from LCs in vitro. Our novel finding was the increase in neutrophils and IL8 within the CL of pregnant cows, suggesting the involvement of IFNT function within the CL toward establishment of pregnancy in cows. The present results suggest that IFNT upregulates neutrophil numbers and function via IL8 on LCs in the CL of early pregnant cows and that both neutrophils and IL8, stimulated by IFNT, are associated with an increase in P4 concentrations during the maternal recognition period in cows.

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Expression, Regulation, and Function of Proprotein Convertase Subtilisin/Kexin Type3 (Furin) in the Peri-Ovulatory Rat Ovary.

Biology of Reproduction ◽

10.1093/biolreprod/81.s1.48 ◽

2009 ◽

Vol 81 (Suppl_1) ◽

pp. 48-48

Author(s):

Brian P. Kelty ◽

Thomas E. Curry

Keyword(s):

Expression Regulation ◽

Proprotein Convertase ◽

Rat Ovary ◽

And Function

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Hormonal regulation and function of an RNA helicase, Ddx5 in corpus luteum of adult Wistar rats

Reproductive Biology ◽

10.1016/j.repbio.2019.05.004 ◽

2019 ◽

Vol 19 (2) ◽

pp. 179-188

Author(s):

Aparamita Pandey ◽

Rudraiah Medhamurthy ◽

Swati Rao ◽

Killivalavan Asaithambi

Keyword(s):

Corpus Luteum ◽

Hormonal Regulation ◽

Wistar Rats ◽

Rna Helicase ◽

And Function

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Does prostacyclin (PGI2) support porcine corpus luteum function? – data from in vitro study

Problems of Endocrinology ◽

10.14341/probl201662549 ◽

2016 ◽

Vol 62 (5) ◽

pp. 49

Author(s):

Magdalena Julia Szymańska ◽

Agnieszka Blitek

Keyword(s):

Endothelial Cells ◽

Mrna Expression ◽

Corpus Luteum ◽

Estrous Cycle ◽

In Vitro Study ◽

Luteal Cells ◽

Enzymatic Isolation ◽

Effective Doses ◽

And Function

Background. Prostacyclin (PGI2) of luteal origin is involved in the control of corpus luteum (CL) development and function in cattle. PGI2 may regulate the process of angiogenesis and may stimulate progesterone (P4) secretion by luteal cells via its specific receptors, PTGIR. In contrast to cattle, the role of PGI2 in the pig CL has not yet been described.Aim. The present study aimed to investigate the effect of PGI2 on 1) P4 secretion by luteal cells, and 2) the expression of angiogenesis-related genes in endothelial cells of the porcine CL.Methods. CL collected from gilts on day 5-7 of the estrous cycle were used for enzymatic isolation of luteal (Experiment 1) and endothelial (Experiment 2) cells. In Exp. 1, cultured luteal cells were incubated with increasing (0, 0.01, 0.1, 1, 5 µM) doses of PGI2 analogues: iloprost (ILO) and carbaprostacyclin (cPGI2) for 8 h. To determine the effective doses of PGI2 analogues, P4 concentration in culture medium was examined by RIA. Thereafter, luteal cells were treated with ILO and cPGI2 at the concentration of 1 and 5 µM in the presence or absence of PTGIR antagonist (CAY10441). After 8 h of incubation the medium was collected for P4 determination. In Exp. 2, isolated endothelial cells were treated for 24 h with ILO and cPGI2 at doses of 1 and 5 µM. Then, cells were collected for analysis of Ang-1 and -2 mRNA expression using qPCR.Results. Both, ILO and cPGI2 affected P4 secretion by luteal cells. Elevated levels of P4 were observed in medium after treatment of luteal cells with 1 µM of ILO and 0.1, 1 and 5 µM of cPGI2 compared with control values (p<0.05). The addition of CAY10441 inhibited the stimulatory effect of ILO on P4 secretion, while did not change P4 production by luteal cells incubated with cPGI2. Moreover, PGI2 analogues differentially affected (p<0.05) the expression of proangiogenic factors. ILO stimulated Ang-2, whereas cPGI2 positively affected Ang-1 mRNA expression in endothelial cells at concentrations of 1 µM and 5 µM, respectively.Conclusion. PGI2 affects P4 secretion during luteal phase of the estrous cycle and may regulate the process of angiogenesis in the porcine CL.

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