Molecular discrimination of Bacillus cereus group species in foods (lettuce, spinach, and kimbap) using quantitative real-time PCR targeting groEL and gyrB

2018 ◽  
Vol 115 ◽  
pp. 312-320 ◽  
Author(s):  
Shuai Wei ◽  
Ramachandran Chelliah ◽  
Byung-Jae Park ◽  
Joong-Hyun Park ◽  
Fereidoun Forghani ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Bacillus Cereus Group ◽  
Pcr Targeting ◽  
Group Species

scholarly journals Detection and quantification of viable Bacillus cereus group species in milk by propidium monoazide quantitative real-time PCR

2016 ◽  
Vol 99 (4) ◽  
pp. 2617-2624 ◽  
Author(s):  
Fernanda Cattani ◽  
Valdir C. Barth ◽  
Jéssica S.R. Nasário ◽  
Carlos A.S. Ferreira ◽  
Sílvia D. Oliveira
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Propidium Monoazide ◽  
Quantitative Real Time Pcr ◽  
Bacillus Cereus Group ◽  
Group Species ◽  
Detection And Quantification

Evaluation of a real-time PCR assay for the differentiation of Bacillus cereus group species

Food Control ◽  
2021 ◽  
Vol 120 ◽  
pp. 107530
Author(s):  
Hendrik Frentzel ◽  
Ylanna Kelner-Burgos ◽  
Carlus Deneke
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Bacillus Cereus Group ◽  
Group Species

Evaluation of a Real-Time PCR Assay for the Detection and Quantification of Bacillus cereus Group Spores in Food

2010 ◽  
Vol 73 (8) ◽  
pp. 1480-1485 ◽  
Author(s):  
JUAN FRANCISCO MARTÍNEZ-BLANCH ◽  
GLORIA SÁNCHEZ ◽  
ESPERANZA GARAY ◽  
ROSA AZNAR
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Isolation ◽  
Food Samples ◽  
Maximum Amount ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Bacillus Cereus Group ◽  
Detection And Quantification

A procedure based on quantitative real-time PCR was evaluated for the detection and quantification of Bacillus cereus spores. Several methods for DNA isolation, such as various heat treatments and germination solutions, were evaluated on spore suspensions of representative strains of the B. cereus group. Overall, the commercially available DNeasy tissue kit yielded the maximum amount of DNA. The procedure also was used to construct calibration curves for different food matrices, with a wide spore quantification range of 5 log units using serial dilutions of spore suspensions of B. cereus CECT 148T. The detection limit for B. cereus in artificially contaminated liquid egg and reconstituted infant formula was about 4 spores per reaction or 60 spores per ml. The newly developed methodology based on the DNeasy tissue kit and an SYBR Green quantitative real-time PCR assay is very suitable for the rapid and accurate detection and quantification of B. cereus group strains and their spores in food samples.

Quantification and differentiation of Bacillus cereus group species in spices and herbs by real-time PCR

Food Control ◽  
2018 ◽  
Vol 83 ◽  
pp. 99-108 ◽  
Author(s):  
Hendrik Frentzel ◽  
Mai Dinh Thanh ◽  
Gladys Krause ◽  
Bernd Appel ◽  
Anneluise Mader
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Bacillus Cereus Group ◽  
Group Species

Development of Rapid Real-Time PCR and Most-Probable-Number Real-Time PCR Assays To Quantify Enterotoxigenic Strains of the Species in the Bacillus cereus Group

2007 ◽  
Vol 70 (12) ◽  
pp. 2774-2781 ◽  
Author(s):  
I-CHEN YANG ◽  
DANIEL YANG-CHIH SHIH ◽  
JAN-YI WANG ◽  
TZU-MING PAN
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Food Samples ◽  
Most Probable Number ◽  
Pcr Assay ◽  
Bacillus Cereus Group ◽  
Probable Number ◽  
Cooked Rice ◽  
Group Detection

Members of the Bacillus cereus group may produce diarrheal enterotoxins and could be potential hazards if they enter the food chain. Therefore, a method capable of detecting all the species in the B. cereus group rather than B. cereus alone is important. We selected nhe as the target and developed a real-time PCR assay to quantify enterotoxigenic strains of the B. cereus group. The real-time PCR assay was evaluated with 60 B. cereus group strains and 28 others. The assay was also used to construct calibration curves for different food matrices and feces. The assay has an excellent quantification capacity, as proved by its linearity (R2 > 0.993), wide dynamic quantification range (102 to 107 CFU/g for cooked rice and chicken, 103 to 107 CFU/ml for milk, and 104 to 107 CFU/g for feces), and adequate relative accuracy (85.5 to 101.1%). For the low-level contaminations, a most-probable-number real-time PCR assay was developed that could detect as low as 100 CFU/ml. Both assays were tested with real food samples and shown to be considerably appropriate for B. cereus group detection and quantification.

scholarly journals Use of the tna Operon as a New Molecular Target for Escherichia coli Detection

2007 ◽  
Vol 73 (19) ◽  
pp. 6321-6325 ◽  
Author(s):  
Camilla Bernasconi ◽  
Giorgio Volponi ◽  
Claudia Picozzi ◽  
Roberto Foschino
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Molecular Target ◽  
Molecular Organization ◽  
Quantitative Real Time Pcr ◽  
High Similarity ◽  
E Coli ◽  
Pcr Targeting

ABSTRACT A quantitative real-time PCR targeting the tnaA gene was studied to detect Escherichia coli and distinguish E. coli from Shigella spp. These microorganisms revealed high similarity in the molecular organization of the tna operon.

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