Annual ryegrass-associated bacteria with potential for plant growth promotion

One of the ways to increase the competitive survivability of rhizobial biofertilizers and thus achieve better plant growth under such conditions is by modifying the rhizospheric environment or community by addition of nonrhizobial nodule-associated bacteria (NAB) that cause better nodulation and plant growth when coinoculated with rhizobia. A study was performed to investigate the most commonly associated nodule-associated bacteria and the rhizospheric microorganisms associated with theFenugreek(Trigonella foenum-graecum) plant. Isolation of nonrhizobial isolates from root nodules ofFenugreekwas carried out along with the rhizospheric isolates. About 64.7% isolates obtained fromFenugreeknodules were gram-negative coccobacilli, 29.41% were gram-positive bacilli, and all rhizospheric isolates except one were gram-positive bacilli. All the isolates were characterized for their plant growth promoting (PGP) activities. Two of the NAB isolates M2N2c and B1N2b (Exiguobacterium sp.) showed maximum positive PGP features. Those NAB isolates when coinoculated with rhizobial strain—S. meliloti, showed plant growth promotion with respect to increase in plant’s root and shoot length, chlorophyll content, nodulation efficiency, and nodule dry weight.

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Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from Lotus parviflorus

Microorganisms ◽

10.3390/microorganisms8040499 ◽

2020 ◽

Vol 8 (4) ◽

pp. 499 ◽

Cited By ~ 2

Author(s):

Ricardo Soares ◽

Jesús Trejo ◽

Maria J. Lorite ◽

Etelvina Figueira ◽

Juan Sanjuán ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Root Nodules ◽

Nifh Gene ◽

Bacterial Strains ◽

Lytic Enzymes ◽

Taxonomic Assignment ◽

Pgp Traits ◽

Associated Bacteria

Lotus spp. are widely used as a forage to improve pastures, and inoculation with elite rhizobial strains is a common practice in many countries. However, only a few Lotus species have been studied in the context of plant-rhizobia interactions. In this study, forty highly diverse bacterial strains were isolated from root nodules of wild Lotus parviflorus plants growing in two field locations in Portugal. However, only 10% of these isolates could nodulate one or more legume hosts tested, whereas 90% were thought to be opportunistic nodule associated bacteria. Phylogenetic studies place the nodulating isolates within the Bradyrhizobium genus, which is closely related to B. canariense and other Bradyrhizobium sp. strains isolated from genistoid legumes and Ornithopus spp. Symbiotic nodC and nifH gene phylogenies were fully consistent with the taxonomic assignment and host range. The non-nodulating bacteria isolated were alpha- (Rhizobium/Agrobacterium), beta- (Massilia) and gamma-proteobacteria (Pseudomonas, Lysobacter, Luteibacter, Stenotrophomonas and Rahnella), as well as some bacteroidetes from genera Sphingobacterium and Mucilaginibacter. Some of these nodule-associated bacteria expressed plant growth promotion (PGP) traits, such as production of lytic enzymes, antagonistic activity against phytopathogens, phosphate solubilization, or siderophore production. This argues for a potential beneficial role of these L. parviflorus nodule-associated bacteria.

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Isolation and Characterization of Nodule Associated Bacteria from Chickpea and their Potential for Plant Growth Promotion

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2017.605.223 ◽

2017 ◽

Vol 6 (5) ◽

pp. 1992-2004 ◽

Cited By ~ 3

Author(s):

Deepak Kumar Koli ◽

Karivaradharajan Swarnalakshmi

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Associated Bacteria ◽

Isolation And Characterization

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Non-Rhizobial Nodule Associated Bacteria (NAB) From Blackgram (Vigna mungo L.) and their possible role in plant growth promotion

Madras Agricultural Journal ◽

10.29321/maj.2019.000291 ◽

2019 ◽

Vol 106 (7-9) ◽

Author(s):

Raja SRT ◽

Sugitha Thangappan ◽

Sivakumar Uthandi

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Vigna Mungo ◽

Associated Bacteria

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Plant Growth Promotion and Biocontrol Mediated by Plant-Associated Bacteria

Plant Microbiome: Stress Response - Microorganisms for Sustainability ◽

10.1007/978-981-10-5514-0_3 ◽

2018 ◽

pp. 45-80 ◽

Cited By ~ 4

Author(s):

Miguel A. Matilla ◽

Tino Krell

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Associated Bacteria

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Epiphytic and endophytic bacteria that promote growth of ethnomedicinal plants in the subtropical forests of Meghalaya, India

Revista de Biología Tropical ◽

10.15517/rbt.v62i4.12138 ◽

2014 ◽

Vol 62 (4) ◽

pp. 1295 ◽

Cited By ~ 14

Author(s):

Fenella Mary War Nongkhla ◽

S. R. Joshi

Keyword(s):

Plant Growth ◽

Plant Growth Promotion ◽

Host Plants ◽

Growth Promotion ◽

Antagonistic Activity ◽

Growth Promoters ◽

Associated Bacteria ◽

Subtropical Forests ◽

Ethnomedicinal Plants ◽

Sacred Forests

The present study was aimed to investigate the endophytic and epiphytic bacteria associated with selected ethnomedicinal plants from the pristine subtropical forests of Meghalaya and analyse them for plant growth promotion and antagonistic ability. This study is an attempt to explore plant associated bacteria which are beneficial to host plants, and thus aid in the conservation of ethnomedicinal plants of the studied subtropical forests, which are dwindling due to exploitation. The plant growth promotion parameters like indole acetic acid (IAA) production, mineral phosphate solubilisation, acid phosphatase activity, presence of 1-aminocyclopropane-1-carboxylic acid deaminase (ACC) gene, nitrogen fixation, cellulose digestion, chitin and pectin were screened among the isolates. The study revealed significant differences in bacterial population not only between the epiphytic and endophytic microhabitats, but also amongst the host plants. Out of the 70 isolated plant associated bacteria, Bacillus sp., Serratia sp., Pseudomonas sp., Pantoea sp., and Lysinibacillus sp. showed potent plant growth promotion properties. Bacillus siamensis C53 and B. subtilis cenB showed significant antagonistic activity against the tested pathogens. This study indicated the isolates inhabiting the plants prevalent in the subtropical sacred forests that could be explored for use as plant growth promoters while practising the cultivation and conservation of ethnomedicinal plants.

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