A spectrophotometric procedure for sialic acid determination in milk employing a flow-batch analysis system with direct heating

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Elisabeth Rey ◽  
Christian Lombart

Abstract The method in which thiobarbituric acid is used for sialic acid determination was adapted to a fully automated procedure, in which the chromogen need not be extracted with an organic solvent. The entire procedure, including hydrolysis, requires 35 min and 25 µl of serum for one analysis, but 30 samples can be analyzed per hour. The procedure, slightly modified, can be used to measure as little as 10 mg of bound sialic acid per liter.


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