Wide color-varying visualization of sulfide with a dual emissive ratiometric fluorescence assay using carbon dots and gold nanoclusters

2020 ◽  
Vol 157 ◽  
pp. 104960 ◽  
Author(s):  
Mohammad Amin Farahmand Nejad ◽  
Arafeh Bigdeli ◽  
M. Reza Hormozi-Nezhad
Keyword(s):  
Carbon Dots ◽  
Gold Nanoclusters ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence

Visual and ratiometric fluorescence detection of Hg2+ based on a dual-emission carbon dots-gold nanoclusters nanohybrid

2018 ◽  
Vol 259 ◽  
pp. 1082-1089 ◽  
Author(s):  
Huizhi Xie ◽  
Jing Dong ◽  
Junling Duan ◽  
Geoffrey I.N. Waterhouse ◽  
Juying Hou ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Carbon Dots ◽  
Gold Nanoclusters ◽  
Ratiometric Fluorescence ◽  
Dual Emission

scholarly journals Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1313
Author(s):  
Pushap Raj ◽  
Seon-yeong Lee ◽  
Tae Yoon Lee
Keyword(s):  
Bladder Cancer ◽  
Hyaluronic Acid ◽  
Carbon Dots ◽  
Early Recognition ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Normal Human ◽  
Fluorescence Biosensor

Bladder cancer is the leading cause of death in patients with genitourinary cancer. An elevated level of hyaluronidase (HAase) was found in bladder cancer, which acts as an important biomarker for the early diagnosis of bladder cancer. Hence, there is a need to develop a simple enzymatic assay for the early recognition of HAase. Herein, we report a simple, sensitive, and ratiometric fluorescence assay for HAase detection under physiological conditions. The fluorescence assay was constructed by the adsorption of cationic carbon dots and positively charged naphthalimide on negatively charged hyaluronic acid and the development of a Förster resonance energy transfer (FRET) mechanism from carbon dots to a naphthalimide fluorophores. The hyaluronidase enzyme cleaves the hyaluronic acid in this assay, and breaking down the FRET mechanism induces ratiometric changes. A detection limit of 0.09 U/mL was achieved, which is less than the HAase level found in normal human body fluids. Moreover, this assay may be used for diagnosing HAase-related diseases.

Cationic Carbon Dots for Modification-Free Detection of Hyaluronidase via an Electrostatic-Controlled Ratiometric Fluorescence Assay

2017 ◽  
Vol 89 (16) ◽  
pp. 8384-8390 ◽  
Author(s):  
Weiqiang Yang ◽  
Jiancong Ni ◽  
Fang Luo ◽  
Wen Weng ◽  
Qiaohua Wei ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence
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