fluorescence biosensor
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2022 ◽  
Vol 199 ◽  
pp. 113863
Author(s):  
Yuan Cheng ◽  
Hongliang Wang ◽  
Yuxin Zhuo ◽  
Dan Song ◽  
Chunsheng Li ◽  
...  

2022 ◽  
Author(s):  
Armando G Salinas ◽  
Jeong Oen Lee ◽  
Shana M Augustin ◽  
Shiliang Zhang ◽  
Tommaso Patriarchi ◽  
...  

Fast-scan cyclic voltammetry (FSCV) is an electrochemical method used to detect dopamine on a subsecond time scale. Recordings using FSCV in freely behaving animals revolutionized the study of behaviors associated with motivation and learning. Despite this advance, FSCV cannot distinguish between catecholamines, which limits its use to brain regions where dopamine is the predominant neurotransmitter. It has also been difficult to detect dopamine in vivo in some striatal subregions with FSCV. Recently, fluorescent biosensors for dopamine were developed, allowing for discrimination between catecholamines. However, the performance of these biosensors relative to FSCV has not been determined. Thus, we compared fluorescent photometry responses of the dopamine biosensor, dLight, with FSCV. We also used dLight photometry to assess changes in tonic and phasic dopamine, which has not been possible with FSCV. Finally, we examined dopamine dynamics during Pavlovian conditioning in striatal subregions, including the dorsolateral striatum where dopamine measurements are challenging with FSCV.


LWT ◽  
2022 ◽  
pp. 113099
Author(s):  
Jie Chen ◽  
Zhuqin Liu ◽  
Jinxin Fang ◽  
Yuanxiu Wang ◽  
Yi Cao ◽  
...  

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2608
Author(s):  
Yuanyuan Gao ◽  
Hong Yu ◽  
Jingjing Tian ◽  
Botao Xiao

Based on a fluorescence “on-off-on” strategy, we fabricated a simple and highly sensitive DNA-based fluorescence biosensor for the detection of micro (mi)RNA from carbon dots (CDs) and graphene oxide (GO) without complicated and time-consuming operations. CDs were successfully synthesized and conjugated to the end of a single-stranded fuel DNA that was adsorbed onto the surface of GO through π-π stacking, resulting in fluorescence quenching. In the presence of the target miRNA let-7a, the fuel DNA was desorbed from the GO surface, and fluorescence was restored through two successive toehold-mediated strand displacement reactions on double-stranded DNA-modified gold nanoparticles. The target miRNA let-7a was recycled, leading to signal amplification. The concentration of let-7a was proportional to the degree of fluorescence recovery. Under optimal conditions, there was a good linear relationship between the relative fluorescence intensity and let-7a concentration in the range of 0.01–1 nM, with a detection limit of 7.8 pM. With its advantages of signal amplification and high biocompatibility, this fluorescence sensing strategy can be applied to the detection of a variety of target miRNAs and can guide the design of novel biosensors with improved properties.


2021 ◽  
Vol 356 ◽  
pp. 129668
Author(s):  
Qingyi Wei ◽  
Peiyao Zhang ◽  
Ting Liu ◽  
Hongbin Pu ◽  
Da-Wen Sun

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Y. Ren ◽  
H. Zhou ◽  
X. Wang ◽  
Q. W. Liu ◽  
X. D. Hou ◽  
...  

ZnS materials have been widely used in fluorescence biosensors to characterize different types of stem cells due to their excellent fluorescence effect. In this study, ZnS was prepared by vulcanizing nano-Zn particles synthesized using a DC arc plasma. The composition and structure of the ZnS materials were studied by X-ray diffraction (XRD), and their functional group information and optical properties were investigated by using IR spectrophotometry and UV-vis spectrophotometry. It has been found that the synthesized materials consist of Zn, cubic ZnS, and hexagonal ZnS according to the vulcanization parameters. Crystalline ZnS was gradually transformed from a cubic to a hexagonal structure, and the cycling properties first increase, then decrease with increasing sulfurization temperature. There is an optimal curing temperature giving the best cycling performance and specific capacity: the material sulfurized thereat mainly consists of cubic β-ZnS phase with a small quantity of Zn and hexagonal α-ZnS. The cubic phase ZnS has better conductivity than hexagonal ZnS, as evinced by electrochemical impedance spectroscopy (EIS). The ZnS (as prepared) shows board absorption, which can be used in fluorescence biosensors in cell imaging systems.


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