Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe

The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/µL of DNA.

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64: Rapid, Species-Specific Detection of Uropathogens from Clinical Urine Specimens Using an Electrochemical Microsensor Array

The Journal of Urology ◽

10.1016/s0022-5347(18)34329-5 ◽

2005 ◽

Vol 173 (4S) ◽

pp. 18-18

Author(s):

Joseph C. Liao ◽

Mitra Mastali ◽

David A. Haake ◽

Bernard M. Churchill

Keyword(s):

Specific Detection ◽

Species Specific ◽

Urine Specimens

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Optimization of droplet digital PCR assays for the type specific detection and quantification of five HPV genotypes, also including additional data on viral load of nine different HPV genotypes in cervical carcinomas

Journal of Virological Methods ◽

10.1016/j.jviromet.2021.114193 ◽

2021 ◽

pp. 114193

Author(s):

Kaliff Malin ◽

Bohr Mordhorst Louise ◽

Helenius Gisela ◽

Karlsson G. Mats ◽

Lillsunde-Larsson Gabriella

Keyword(s):

Viral Load ◽

Additional Data ◽

Digital Pcr ◽

Droplet Digital Pcr ◽

Specific Detection ◽

Cervical Carcinomas ◽

Hpv Genotypes ◽

Pcr Assays ◽

Detection And Quantification

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Back-trajectory modelling and DNA-based species-specific detection methods allow tracking of fungal spore transport in air masses

The Science of The Total Environment ◽

10.1016/j.scitotenv.2016.07.034 ◽

2016 ◽

Vol 571 ◽

pp. 658-669 ◽

Cited By ~ 10

Author(s):

Agnieszka Grinn-Gofroń ◽

Magdalena Sadyś ◽

Joanna Kaczmarek ◽

Aleksandra Bednarz ◽

Sylwia Pawłowska ◽

...

Keyword(s):

Fungal Spore ◽

Detection Methods ◽

Back Trajectory ◽

Specific Detection ◽

Air Masses ◽

Species Specific ◽

Trajectory Modelling

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Development and Application of a New PCR Method for Detection of Blumeria graminis f. sp. tritici

Journal of Molecular Microbiology and Biotechnology ◽

10.1159/000494432 ◽

2018 ◽

Vol 28 (3) ◽

pp. 137-146 ◽

Cited By ~ 1

Author(s):

Adam Kuzdraliński ◽

Hubert Szczerba ◽

Anna Kot ◽

Agnieszka Ostrowska ◽

Michał Nowak ◽

...

Keyword(s):

Dna Sequences ◽

Puccinia Triticina ◽

Blumeria Graminis ◽

Pyrenophora Tritici Repentis ◽

Field Samples ◽

Expected Length ◽

Pcr Assays ◽

Primer Sets ◽

Wheat Leaves ◽

Species Specific

We developed new PCR assays that target beta-tubulin (TUB2) and 14 alpha-demethylase (CYP51) genes and used them for the species-specific detection of Blumeria graminis f. sp. tritici (Bgt). Based on fungi DNA sequences available in the NCBI (National Center for Biotechnology Information) GenBank database we developed simplex and duplex PCR assays. The specificities of the primer sets were evaluated using environmental samples of wheat leaves collected during the 2015/2016 growing season across Poland. Primer sets LidBg17/18 and LidBg21/22 strongly amplified fragments of the expected length for all 67 tested samples. Primer specificity was confirmed using field samples of Zymoseptoria tritici, Puccinia triticina (syn. P. recondita f. sp. tritici), P. striiformis f. sp. tritici, and Pyrenophora tritici-repentis.

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