Application of the VPp1 bacteriophage combined with a coupled enzyme system in the rapid detection of Vibrio parahaemolyticus

2014 ◽  
Vol 98 ◽  
pp. 99-104 ◽  
Author(s):  
Yong Peng ◽  
Yanqiu Jin ◽  
Hong Lin ◽  
Jingxue Wang ◽  
Muhammad Naseem Khan
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Rapid Detection ◽  
Enzyme System

Development and evaluation of a sensitive recombinase aided amplification assay for rapid detection of Vibrio parahaemolyticus

2022 ◽  
pp. 106404
Author(s):  
Zhi-shan Feng ◽  
Jing-yi Li ◽  
Jing-yun Zhang ◽  
Feng-yu Li ◽  
Hong-xia Guan ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Rapid Detection ◽  
Amplification Assay

Rapid Detection of Vibrio parahaemolyticus in Shellfish by Real-Time Recombinase Polymerase Amplification

2018 ◽  
Vol 11 (8) ◽  
pp. 2076-2084 ◽  
Author(s):  
Peng Zhu ◽  
Weifang Gao ◽  
Hailong Huang ◽  
Jinpo Jiang ◽  
Xianfeng Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Vibrio Parahaemolyticus ◽  
Rapid Detection ◽  
Recombinase Polymerase Amplification

scholarly journals Development of a Loop-Mediated Isothermal Amplification Assay for Sensitive and Rapid Detection of the tdh and trh Genes of Vibrio parahaemolyticus and Related Vibrio Species

2009 ◽  
Vol 76 (3) ◽  
pp. 820-828 ◽  
Author(s):  
Wataru Yamazaki ◽  
Yuko Kumeda ◽  
Naoaki Misawa ◽  
Yoshitsugu Nakaguchi ◽  
Mitsuaki Nishibuchi
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Rapid Detection ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Virulence Determinants ◽  
Bacterial Strains ◽  
Phenotypic Differences ◽  
Loop Mediated Isothermal Amplification ◽  
Vibrio Mimicus ◽  
Thermostable Direct Hemolysin

ABSTRACT Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are the major virulence determinants of Vibrio parahaemolyticus. TRH is further differentiated into TRH1 and TRH2 on the basis of genetic and phenotypic differences. We developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for sensitive and rapid detection of the tdh, trh1, and trh2 genes of V. parahaemolyticus. The LAMP assay was designed for both combined and individual detection of the tdh, trh1, and trh2 genes and combined detection of the trh1 and trh2 genes. Our results showed that it gave the same results as DNA probes and conventional PCR assays for 125 strains of V. parahaemolyticus, 3 strains of Grimontia hollisae, and 2 strains of Vibrio mimicus carrying the tdh, trh1, and trh2 genes in various combinations. No LAMP products were detected for any of the 20 bacterial strains lacking the tdh, trh1, and trh2 genes. The sensitivities of the LAMP assay for detection of tdh-, trh1-, and trh2-carrying V. parahaemolyticus strains in spiked shrimp samples were 0.8, 21.3, and 5.0 CFU per LAMP reaction tube, respectively. Starting with DNA extraction from a single colony and from spiked shrimp samples, the LAMP assay required only 27 to 60 min and less than 80 min, respectively. This is the first report of a rapid and specific LAMP assay for detection and differentiation of the tdh, trh1, and trh2 genes of V. parahaemolyticus and related Vibrio species.

scholarly journals Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus

2019 ◽  
Vol 42 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Sara Hash ◽  
M. Pilar Martinez-Viedma ◽  
Fred Fung ◽  
Jee Eun Han ◽  
Paul Yang ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Vibrio Parahaemolyticus ◽  
Rapid Detection ◽  
Nuclear Magnetic
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