Identification and application of a neutralizing epitope within alpha-hemolysin using human serum antibodies elicited by vaccination

The nature of serum, secretory, and urinary antibodies reactive with indigenous bacteria was investigated. The immunoglobulin class of serum antibodies to Streptococcus mitis, Streptococcus salivarius, an enterococcus, Veillonella, and Escherichia coli was different from that of the parotid secretion, whole saliva, and urine of the same normal subject. While serum antibodies to these bacteria were primarily of the IgM class, those of the secretions and urine were associated almost exclusively with the secretory IgA and IgG immunoglobulins, respectively. These results suggest that secretory and urinary antibodies are synthesized independently of serum antibodies.

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The two-cross immunodiffusion technique: Diffusion coefficients and precipitating titers of IgG in human serum and rabbit serum antibodies

Analytical Biochemistry ◽

10.1016/s0003-2697(79)80123-2 ◽

1979 ◽

Vol 93 ◽

pp. 103-114 ◽

Cited By ~ 11

Author(s):

Biserka Pokrić ◽

Zvonimir Pučar

Keyword(s):

Human Serum ◽

Diffusion Coefficients ◽

Rabbit Serum ◽

Serum Antibodies

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SERUM ANTIBODIES IN STAPHYLOCOCCAL DISEASE

PEDIATRICS ◽

10.1542/peds.33.1.63 ◽

1964 ◽

Vol 33 (1) ◽

pp. 63-70

Author(s):

Paul G. Quie ◽

Lewis W. Wannamaker

Keyword(s):

Antibody Response ◽

Skin Infection ◽

Normal Population ◽

Serum Antibody ◽

Serum Antibodies ◽

Population Groups ◽

Alpha Hemolysin ◽

General Similarity ◽

Antibody Titers ◽

Staphylococcal Pneumonia

Serum antibody titers to the staphylococcal Muller factor and alpha-hemolysin were determined in population groups of various ages and in maternal-cord serum pairs. There was evidence for placental transmission of antibodies and after 2 years of age most persons demonstrated antibody to both of these staphylococcal extracellular products. Antibody titers in patients with staphylococcal lesions were usually in the upper range of those found in the normal population of similar age, but no striking or consistent elevation of titers was observed in association with or following staphylococcal disease. Exceptions, due in part perhaps to age, were found in patients with staphylococcal pneumonia whose sera often contained no measurable antibody and in certain patients with recurrent skin infection whose sera contained alpha-hemolysin antibodies but failed to demonstrate Muller factor antibodies. The general similarity of antibody titers in patients with staphylococcal lesions to those in normal persons suggests that the antibody response to staphylococcal Muller factor and to alpha-hemolysin is little different after a staphylococcal lesion than after nasal or cutaneous colonization. These findings emphasize that, in addition to antibodies to toxins, presently ill-defined cellular bactericidal mechanisms are necessary for complete defense against staphylococcal disease.

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