scholarly journals Quantification of DNA repair capacity in whole blood of patients with head and neck cancer and healthy donors by comet assay

2008 ◽  
Vol 650 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Daniel T. Saha ◽  
Bruce J. Davidson ◽  
Antai Wang ◽  
Allison J. Pollock ◽  
Roy A. Orden ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Comet Assay ◽  
Head And Neck ◽  
Neck Cancer ◽  
Whole Blood ◽  
Repair Capacity ◽  
Dna Repair Capacity ◽  
Healthy Donors

scholarly journals DNA Repair Capacity and the Risk of Head and Neck Cancer

10.5772/31555 ◽  
2012 ◽  
Author(s):  
Marcin Szaumkessel ◽  
Wojciech Gawcki ◽  
Krzysztof Szyfter
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Repair Capacity ◽  
Dna Repair Capacity

DNA Damage and Repair of Head and Neck Cancer Cells after Radio- and Chemotherapy

2009 ◽  
Vol 64 (7-8) ◽  
pp. 601-610 ◽  
Author(s):  
Pawel Rusin ◽  
Anna Walczak ◽  
Anita Zwierzchlejska ◽  
Jurek Olszewski ◽  
Alina Morawiec-Bajda ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Cancer Cell Line ◽  
Repair Capacity ◽  
Dna Damage And Repair ◽  
Γ Radiation

DNA repair is critical for successful chemo- and radiotherapy of human tumours, because their genotoxic sensitivity may vary in different types of cancer cells. In this study we have compared DNA damage and the efficiency of its repair after genotoxic treatment with hydrogen peroxide, cisplatin and γ-radiation of head and neck squamous cell carcinoma (HNSCC). Lymphocytes and tissue cells from biopsies of 37 cancer patients and 35 healthy donors as well as the HTB-43 larynx cancer cell line were employed. The cell sensitivity to genotoxic treatment was estimated by the MTT survival assay. The extent of DNA damage and efficiency of its repair was examined by the alkaline comet assay. Among the examined treatments, we found that HNSCC cells were the most sensitive to γ-radiation and displayed impaired DNA repair. In particular, DNA damage was repaired less effectively in cells from HNSCC metastasis than healthy controls. In conclusion, our results suggest that the different genotoxic sensitivity of HNSCC cells may depend on their DNA repair capacity what in turn may be connected with the effectiveness of head and neck cancer therapy.

scholarly journals DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests.

2003 ◽  
Vol 50 (1) ◽  
pp. 181-190 ◽  
Author(s):  
Olena Palyvoda ◽  
Joanna Polańska ◽  
Andrzej Wygoda ◽  
Joanna Rzeszowska-Wolny
Keyword(s):  
Dna Damage ◽  
Head And Neck Cancer ◽  
Comet Assay ◽  
Cancer Patient ◽  
Head And Neck ◽  
Patient Group ◽  
Neck Cancer ◽  
Dna Damage And Repair ◽  
Damage Level ◽  
Healthy Donors

A population study is reported in which the DNA damage induced by g-radiation (2 Gy) and the kinetics of the subsequent repair were estimated by the comet and micronucleus assays in isolated lymphocytes of 82 healthy donors and patients with head and neck cancer before radiotherapy. The parameters of background and radiation-induced DNA damage, rate of repair, and residual non-repaired damage were measured by comet assay, and the repair kinetics for every donor were computer-fitted to an exponential curve. The level of background DNA damage before irradiation measured by comet assay as well as the level of micronuclei were significantly higher in the head and neck cancer patient group than in the healthy donors, while the parameters of repair were widely scattered in both groups. Cancer patient group contained significantly more individuals, whose irradiated lymphocytes showed high DNA damage, low repair rate and high non-repaired DNA damage level. Lymphocytes of donors belonging to this subgroup showed significantly lower inhibition of cell cycle after irradiation.

scholarly journals DNA Repair in Lymphoblastoid Cell Lines From Patients With Head and Neck Cancer

1999 ◽  
Vol 125 (2) ◽  
pp. 185 ◽  
Author(s):  
Erich M. Sturgis ◽  
Gary L. Clayman ◽  
Yongli Guan ◽  
Zhaozheng Guo ◽  
Qingyi Wei
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cell Lines ◽  
Neck Cancer ◽  
Lymphoblastoid Cell Lines

DNA repair gene polymorphisms and risk of head and neck cancer in the Tunisian population

2013 ◽  
Vol 43 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Rim Khlifi ◽  
Imen Kallel ◽  
Bouthaina Hammami ◽  
Amel Hamza-Chaffai ◽  
Ahmed Rebai
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Gene Polymorphisms ◽  
Tunisian Population ◽  
Repair Gene ◽  
Dna Repair Gene

DNA repair- and apoptosis-pathways to regulate response to chemo-radiotherapy (CRT) in patients (p) with locally advanced head and neck cancer (HNC).

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e17025-e17025
Author(s):  
Beatriz Cirauqui ◽  
Vanesa Quiroga ◽  
Imane Chaib ◽  
Belen Sanchez ◽  
Niki Karachaliou ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Locally Advanced ◽  
Advanced Head ◽  
Apoptosis Pathways

Role of impaired DNA repair in genotoxic susceptibility of patients with head and neck cancer

2008 ◽  
Vol 25 (5) ◽  
pp. 489-497 ◽  
Author(s):  
Pawel Rusin ◽  
Jurek Olszewski ◽  
Alina Morawiec-Bajda ◽  
Karolina Przybylowska ◽  
Dariusz Kaczmarczyk ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer

scholarly journals Genetic polymorphisms in key DNA repair genes and risk of head and neck cancer in a Chinese population

2012 ◽  
Vol 3 (4) ◽  
pp. 719-724 ◽  
Author(s):  
HUA YUAN ◽  
HUIZHANG LI ◽  
HONGXIA MA ◽  
YUMING NIU ◽  
YUNONG WU ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Genetic Polymorphisms ◽  
Neck Cancer ◽  
Chinese Population ◽  
Dna Repair Genes ◽  
Repair Genes

The Expression of Checkpoint and DNA Repair Genes in Head and Neck Cancer as Possible Predictive Factors

2016 ◽  
Vol 23 (2) ◽  
pp. 253-264 ◽  
Author(s):  
Orsolya Rusz ◽  
Margit Pál ◽  
Éva Szilágyi ◽  
László Rovó ◽  
Zoltán Varga ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Predictive Factors ◽  
Dna Repair Genes ◽  
Repair Genes
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