Novel bone graft model using bead-cell sheets composed of tricalcium phosphate beads and bone marrow cells

Abstract The availability of cloned lines of bone marrow stromal cells could facilitate the analysis of their role in hemopoietic cell development. The 266AD cell line was isolated from a colony of lipid-accumulating bone marrow cells growing in a collagen gel. 266AD cells have subsequently been maintained by passage in tissue culture plastic flasks about every 10 days for greater than 10 mo. Subconfluent cultures of cells are fibroblast-appearing, but in confluent cell sheets, prominent foci of lipid-containing cells develop in both uncloned and four separate cloned cell lines. Supernatants from confluent cultures containing lipid-laden cells contain granulocyte- macrophage colony-stimulating activity (GM-CSA) for normal bone marrow cells and can induce differentiation of Abelson virus transformed murine promonocytic leukemia cells. 266AD cells were originally isolated in the presence of hydrocortisone, but hydrocortisone is not necessary for lipogenesis to occur. Growth of bone marrow cells in a collagen gel matrix provided a way to isolate stromal cells, and the 266AD cell line provides a means to examine the relationships between stromal cell lipogenesis and regulation of granulopoiesis.

Download Full-text

Bone tissue engineering based on bead–cell sheets composed of calcium phosphate beads and bone marrow cells

Materials Science and Engineering C ◽

10.1016/j.msec.2003.11.010 ◽

2004 ◽

Vol 24 (3) ◽

pp. 437-440 ◽

Cited By ~ 16

Author(s):

Katsuko S. Furukawa ◽

Shunsuke Miyauchi ◽

Daisuke Suzuki ◽

Yoshikazu Umezu ◽

Tsuneo Shinjo ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Marrow ◽

Calcium Phosphate ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Marrow Cells ◽

Cell Sheets ◽

Marrow Cells

Download Full-text

Lipid accumulation and production of colony-stimulating activity by the 266AD cell line derived from mouse bone marrow

Blood ◽

10.1182/blood.v61.2.397.bloodjournal612397 ◽

1983 ◽

Vol 61 (2) ◽

pp. 397-402

Author(s):

DL Hines

Keyword(s):

Bone Marrow ◽

Cell Line ◽

Stromal Cells ◽

Bone Marrow Cells ◽

Collagen Gel ◽

Mouse Bone Marrow ◽

Cell Sheets ◽

Colony Stimulating Activity ◽

Confluent Cell ◽

Marrow Cells

The availability of cloned lines of bone marrow stromal cells could facilitate the analysis of their role in hemopoietic cell development. The 266AD cell line was isolated from a colony of lipid-accumulating bone marrow cells growing in a collagen gel. 266AD cells have subsequently been maintained by passage in tissue culture plastic flasks about every 10 days for greater than 10 mo. Subconfluent cultures of cells are fibroblast-appearing, but in confluent cell sheets, prominent foci of lipid-containing cells develop in both uncloned and four separate cloned cell lines. Supernatants from confluent cultures containing lipid-laden cells contain granulocyte- macrophage colony-stimulating activity (GM-CSA) for normal bone marrow cells and can induce differentiation of Abelson virus transformed murine promonocytic leukemia cells. 266AD cells were originally isolated in the presence of hydrocortisone, but hydrocortisone is not necessary for lipogenesis to occur. Growth of bone marrow cells in a collagen gel matrix provided a way to isolate stromal cells, and the 266AD cell line provides a means to examine the relationships between stromal cell lipogenesis and regulation of granulopoiesis.

Download Full-text

Nanomaterial N-CP/DLPLG as potent1onal tissue graft in osteoreparation in combination with bone marrow cells on subcutaneous implantation model

Hemijska industrija ◽

10.2298/hemind0803205j ◽

2008 ◽

Vol 62 (3) ◽

pp. 205-210 ◽

Cited By ~ 3

Author(s):

Jelena Janicijevic ◽

Stevo Najman ◽

Nenad Ignjatovic ◽

Vojin Savic ◽

Jelena Kocic ◽

...

Keyword(s):

Bone Marrow ◽

Calcium Phosphate ◽

Bone Graft ◽

Bone Marrow Cells ◽

Subcutaneous Implantation ◽

Tissue Scaffold ◽

Tissue Graft ◽

Good Biocompatibility ◽

Implantation Model ◽

Marrow Cells

The need for bone graft materials in osteoreparation is tremendous. Many researches have shown that calcium-phosphate bioceramics have good biocompatibility and osteoconductivity. We used nanocomposite biomaterial calcium phosphate coated with poly (dl-lactide-co-glycolide) or N-CP/DLPLG. The goal of this investigation was to examine weather N-CP/DLPLG has ability to sustain growth of bone marrow cells after subcutaneous implantation in Balb/c mice. For that purpose N-CP/DLPLG implants with and without bone marrow cells (control) were made. Implants were extracted after eight days and eight weeks. In implants loaded with bone marrow cells after eight days and eight weeks we observed fields rich in cells, angiogenesis and collagen genesis. These results showed that N-CP/DLPLG has property of tissue scaffold which sustain bone marrow cells growth and collagen production. This represents a good way for further examination of N-CP/DLPLG as potentional tissue scaffold in osteoreparation.

Download Full-text