In vitro degradation of novel bioactive polycaprolactone—20% tricalcium phosphate composite scaffolds for bone engineering

In order to improvedβ-TCP biocompatibility and cell growth, was chosen to modify β-TCP matrices to produce a γ-PGA/β-TCP composite biomaterial. Then, the morphology, water uptake and retention abilities,in vitrodegradation property in the simulated medium, cytotoxicity of this novel γ-PGA/β-TCP composite is investigated. SEM shows that the γ-PGA/β-TCP composite has a porous structure. By increasing the percentage ofγ-PGA from 0% to 50%, the swelling ratio of the composite s was enhanced from 9.0%to 297%. These data suggested that the surface hydrophilicity, water absorption rate, and swelling ratio were improved by adding γ-PGA to the composite. In the cytocompatibility test, the density of MC3T3-E1 preosteoblasts cells on the PTCP1:1 leachates was almost 110% higher than that on the controls on day 3. Therefore, the γ-PGA/β-TCP composite scaffolds, due to their better hydrophilicity, cytocompatibility, and porous structure, are very promising biomaterials for tissure engineering applications.

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Three-Dimensional Hierarchical Composite Scaffolds Consisting of Polycaprolactone, β-Tricalcium Phosphate, and Collagen Nanofibers: Fabrication, Physical Properties, and In Vitro Cell Activity for Bone Tissue Regeneration

Biomacromolecules ◽

10.1021/bm1013052 ◽

2011 ◽

Vol 12 (2) ◽

pp. 502-510 ◽

Cited By ~ 89

Author(s):

MyungGu Yeo ◽

Hyeongjin Lee ◽

GeunHyung Kim

Keyword(s):

Physical Properties ◽

Bone Tissue ◽

Tissue Regeneration ◽

Tricalcium Phosphate ◽

Cell Activity ◽

Three Dimensional ◽

Bone Tissue Regeneration ◽

Composite Scaffolds

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2F31 In vitro study on collagen/tricalcium phosphate composite scaffolds for bone tissue regeneration

The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME ◽

10.1299/jsmebio.2014.26.497 ◽

2014 ◽

Vol 2014.26 (0) ◽

pp. 497-498

Author(s):

Takaaki ARAHIRA ◽

Mitsugu TODO

Keyword(s):

Bone Tissue ◽

Tissue Regeneration ◽

Tricalcium Phosphate ◽

In Vitro Study ◽

Vitro Study ◽

Bone Tissue Regeneration ◽

Composite Scaffolds

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In vitro bone engineering based on polycaprolactone and polycaprolactone–tricalcium phosphate composites

Polymer International ◽

10.1002/pi.2138 ◽

2007 ◽

Vol 56 (3) ◽

pp. 333-342 ◽

Cited By ~ 61

Author(s):

Yefang Zhou ◽

Dietmar W Hutmacher ◽

Sae-Lim Varawan ◽

Tit Meng Lim

Keyword(s):

Tricalcium Phosphate ◽

Bone Engineering

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In Vitro Degradation of Poly(caprolactone)/nHA Composites

Journal of Nanomaterials ◽

10.1155/2014/802435 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 51

Author(s):

Esperanza Díaz ◽

Iban Sandonis ◽

María Blanca Valle

Keyword(s):

Mechanical Properties ◽

Degradation Products ◽

Degradation Process ◽

Porous Scaffolds ◽

Degradation Behavior ◽

Compressive Properties ◽

Composite Scaffolds ◽

In Vitro Degradation ◽

Poly Caprolactone

The degradation behavior and mechanical properties of polycaprolactone/nanohydroxyapatite composite scaffolds are studied in phosphate buffered solution (PBS), at 37°C, over 16 weeks. Under scanning electron microscopy (SEM), it was observed that the longer the porous scaffolds remained in the PBS, the more significant the thickening of the pore walls of the scaffold morphology was. A decrease in the compressive properties, such as the modulus and the strength of the PCL/nHA composite scaffolds, was observed as the degradation experiment progressed. Samples with high nHA concentrations degraded more significantly in comparison to those with a lower content. Pure PCL retained its mechanical properties comparatively well in the study over the period of degradation. After the twelfth week, the results obtained by GPC analysis indicated a significant reduction in their molecular weight. The addition of nHA particles to the scaffolds accelerated the weight loss of the composites and increased their capacity to absorb water during the initial degradation process. The addition of these particles also affected the degradation behavior of the composite scaffolds, although they were not effective at compensating the decrease in pH prompted by the degradation products of the PCL.

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