In vitro evaluation of actively targetable superparamagnetic nanoparticles to the folate receptor positive cancer cells

2016 ◽  
Vol 69 ◽  
pp. 1147-1158 ◽  
Author(s):  
Rozita Nasiri ◽  
Javad Hamzehalipour Almaki ◽  
Ani Binti Idris ◽  
Fadzilah Adibah Abdul Majid ◽  
Mahtab Nasiri ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Folate Receptor ◽  
Superparamagnetic Nanoparticles ◽  
In Vitro Evaluation

SYSTEMATIC IN VITRO EVALUATION OF SURVIVIN DIRECTED ANTISENSE OLIGODEOXYNUCLEOTIDES IN BLADDER CANCER CELLS

2004 ◽  
Vol 171 (6 Part 1) ◽  
pp. 2471-2476 ◽  
Author(s):  
SUSANNE FUESSEL ◽  
BERND KUEPPERS ◽  
SHUANGLI NING ◽  
MATTHIAS KOTZSCH ◽  
KAI KRAEMER ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Antisense Oligodeoxynucleotides ◽  
In Vitro Evaluation ◽  
Bladder Cancer Cells

scholarly journals Synthesis, Characterization and in Vitro Evaluation of Manganese Ferrite (MnFe2O4) Nanoparticles for Their Biocompatibility with Murine Breast Cancer Cells (4T1)

Molecules ◽  
2016 ◽  
Vol 21 (3) ◽  
pp. 312 ◽  
Author(s):  
Samikannu kanagesan ◽  
Sidek Aziz ◽  
Mansor Hashim ◽  
Ismayadi Ismail ◽  
Subramani Tamilselvan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Manganese Ferrite ◽  
In Vitro Evaluation ◽  
Mnfe2o4 Nanoparticles

In vitro evaluation of newly developed chalcone analogues in human cancer cells

2000 ◽  
Vol 46 (4) ◽  
pp. 305-312 ◽  
Author(s):  
Rosa De Vincenzo ◽  
Cristiano Ferlini ◽  
Mariagrazia Distefano ◽  
Cristiana Gaggini ◽  
Antonella Riva ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
In Vitro Evaluation ◽  
Human Cancer Cells

scholarly journals Design, synthesis and in vitro evaluation against human cancer cells of 5-methyl-5-styryl-2,5-dihydrofuran-2-ones, a new series of goniothalamin analogues

2013 ◽  
Vol 21 (17) ◽  
pp. 5107-5117 ◽  
Author(s):  
Marjorie Bruder ◽  
Débora Barbosa Vendramini-Costa ◽  
João Ernesto de Carvalho ◽  
Ronaldo Aloise Pilli
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Design Synthesis ◽  
In Vitro Evaluation ◽  
Human Cancer Cells

scholarly journals Optimisation of Folate-Mediated Liposomal Encapsulated Arsenic Trioxide for Treating HPV-Positive Cervical Cancer Cells In Vitro

2019 ◽  
Vol 20 (9) ◽  
pp. 2156 ◽  
Author(s):  
Akhtar ◽  
Ghali ◽  
Wang ◽  
Bell ◽  
Li ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Folic Acid ◽  
Cancer Cells ◽  
Arsenic Trioxide ◽  
Folate Receptor ◽  
Hpv Infection ◽  
Cytotoxicity Studies ◽  
Cervical Cancer Cells ◽  
Infected Cells

High-risk human papilloma virus (HPV) infection is directly associated with cervical cancer development. Arsenic trioxide (ATO), despite inducing apoptosis in HPV-infected cervical cancer cells in vitro, has been compromised by toxicity and poor pharmacokinetics in clinical trials. Therefore, to improve ATO’s therapeutic profile for HPV-related cancers, this study aims to explore the effects of length of ligand spacers of folate-targeted liposomes on the efficiency of ATO delivery to HPV-infected cells. Fluorescent ATO encapsulated liposomes with folic acid (FA) conjugated to two different PEG lengths (2000 Da and 5000 Da) were synthesised, and their cellular uptake was examined for HPV-positive HeLa and KB and HPV-negative HT-3 cells using confocal microscopy, flow cytometry, and spectrophotometer readings. Cellular arsenic quantification and anti-tumour efficacy was evaluated through inductively coupled plasma-mass spectrometry (ICP-MS) and cytotoxicity studies, respectively. Results showed that liposomes with a longer folic acid-polyethylene glycol (FA-PEG) spacer (5000 Da) displayed a higher efficiency in targeting folate receptor (FR) + HPV-infected cells without increasing any inherent cytotoxicity. Targeted liposomally delivered ATO also displayed superior selectivity and efficiency in inducing higher cell apoptosis in HPV-positive cells per unit of arsenic taken up than free ATO, in contrast to HT-3. These findings may hold promise in improving the management of HPV-associated cancers.

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