Preparation and characterization of PLA/PCL/HA composite scaffolds using indirect 3D printing for bone tissue engineering

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

Download Full-text

Preparation and characterization of nano-sized hydroxyapatite/alginate/chitosan composite scaffolds for bone tissue engineering

Materials Science and Engineering C ◽

10.1016/j.msec.2015.04.033 ◽

2015 ◽

Vol 54 ◽

pp. 20-25 ◽

Cited By ~ 131

Author(s):

Hye-Lee Kim ◽

Gil-Yong Jung ◽

Jun-Ho Yoon ◽

Jung-Suk Han ◽

Yoon-Jeong Park ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Composite Scaffolds ◽

Chitosan Composite

Download Full-text

A review on the 3D printing of composite scaffolds for bone tissue engineering

10.1016/b978-0-12-823893-6.00001-2 ◽

2022 ◽

pp. 201-241

Author(s):

Catherine Seo ◽

Chaoxing Zhang ◽

Huinan Liu

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Composite Scaffolds

Download Full-text

Synthesis and characterization of CaP/Col composite scaffolds for load-bearing bone tissue engineering

Composites Part B Engineering ◽

10.1016/j.compositesb.2014.03.008 ◽

2014 ◽

Vol 62 ◽

pp. 242-248 ◽

Cited By ~ 14

Author(s):

C.C. Zhou ◽

X.J. Ye ◽

Y.J. Fan ◽

F.Z. Qing ◽

H.J. Chen ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Synthesis And Characterization ◽

Composite Scaffolds ◽

Load Bearing

Download Full-text

Synthesis and characterization of nano-hydroxyapatite rods/poly(l-lactide acid) composite scaffolds for bone tissue engineering

Composites Part A Applied Science and Manufacturing ◽

10.1016/j.compositesa.2008.05.018 ◽

2008 ◽

Vol 39 (10) ◽

pp. 1589-1596 ◽

Cited By ~ 118

Author(s):

E. Nejati ◽

H. Mirzadeh ◽

M. Zandi

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Synthesis And Characterization ◽

Composite Scaffolds

Download Full-text

Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold

RSC Advances ◽

10.1039/c6ra17718b ◽

2016 ◽

Vol 6 (112) ◽

pp. 110557-110565 ◽

Cited By ~ 49

Author(s):

Yinxian Yu ◽

Sha Hua ◽

Mengkai Yang ◽

Zeze Fu ◽

Songsong Teng ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Composite Scaffold ◽

Tissue Engineering Scaffold ◽

Fabrication And Characterization

A composite scaffold was fabricated with a method involving both electrospinning and 3D printing to give microscale pores and good mechanical properties. Biocompatibility and cell infiltration on the scaffold was evaluated by an in vitro study.

Download Full-text