Molecular identification and azole susceptibility testing of Aspergillus section Fumigati isolated from soil samples in Lebanon

2021 ◽  
pp. 101242
Author(s):  
Sara Khalife ◽  
Agustin Resendiz-Sharpe ◽  
Katrien Lagrou ◽  
Emilie Fréalle
Keyword(s):  
Molecular Identification ◽  
Susceptibility Testing ◽  
Soil Samples ◽  
Aspergillus Section

scholarly journals Distribution of Aspergillus Section Flavi among Field Soils from the Four Agroecological Zones of the Republic of Bénin, West Africa

Plant Disease ◽  
2002 ◽  
Vol 86 (4) ◽  
pp. 434-439 ◽  
Author(s):  
K. F. Cardwell ◽  
P. J. Cotty
Keyword(s):  
Cropping Systems ◽  
Fermentation Medium ◽  
Soil Samples ◽  
Aspergillus Section Flavi ◽  
The North ◽  
Agroecological Zones ◽  
Republic Of Benin ◽  
The Republic ◽  
Aflatoxin B ◽  
Aspergillus Section

Certain members of Aspergillus section Flavi produce carcinogenic and immunotoxic metabo-lites called aflatoxins. These fungi perennate in soils and infect maize grain in the field and in storage. The distribution of Aspergillus section Flavi across the four different agroecologies of Bénin Republic was determined. The four agroecological zones range from humid equatorial tropics in the south to the dry savanna near the Sahara desert in the north. Soil samples collected in 1994 to 1996 from 44 different maize fields in Bénin were assayed over 3 years (88 samples total) for fungi in Aspergillus section Flavi. All soils tested contained A. flavus. Isolates (1,454 total) were collected by dilution plate from the soils and existed in populations ranging from <10 to >200 CFU/g of soil. CFU counts did not differ from year to year or change significantly with cropping systems within a zone, but differed significantly among zones. Incidence of A. flavus strain isolations varied from south to north, with greater number of CFU of L strain isolates in southern latitudes and higher numbers of CFU of S strain isolates found in the north. The L strain isolates occurred in 81 of 88 samples, whereas S strain isolates were in only 41 of 88 soil samples. Of 96 L strain isolates tested, 44% produced aflatoxins. Only B toxins were produced, and toxigenic isolates averaged over 100 μg of aflatoxin B1 per 70 ml of fermentation medium (~1.4 ppm). All S strain isolates produced both B and G aflatoxins, averaging over 557 μg of aflatoxin B1 per 70 ml (8 ppm) and 197 μg of aflatoxin G1 per 70 ml of fermentation me- dium (2.8 ppm). A. parasiticus and A. tamarii were present in less than 10% of the fields and were not associated with any particular agroecological zone.

scholarly journals Molecular identification and antifungal susceptibility testing of Pucciniomycotina red yeast clinical isolates from Rio de Janeiro, Brazil

2019 ◽  
Vol 51 (1) ◽  
pp. 95-98
Author(s):  
Fabio Brito-Santos ◽  
Maria Helena Galdino Figueiredo-Carvalho ◽  
Rowena Alves Coelho ◽  
Jean Carlos Almeida de Oliveira ◽  
Raissa Vieira Monteiro ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Rio De Janeiro ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Clinical Isolates ◽  
Red Yeast

scholarly journals Real life turnaround time of blood cultures in the clinical microbiology laboratory: results of the first Italian survey, May 2015

2016 ◽  
Vol 31 (3) ◽  
Author(s):  
Fabio Arena ◽  
Marta Argentieri ◽  
Paola Bernaschi ◽  
Giacomo Fortina ◽  
Vesselina Kroumova ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Susceptibility Testing ◽  
Real Life ◽  
Turnaround Time ◽  
High Rate ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Italian Survey ◽  
Multiple Outcome ◽  
Reported Data

<em>Background and aims</em>: Blood culture (BC) results are essential to guide antimicrobial chemotherapy for patients with sepsis. However, BC is a time-consuming exam, which can take several days. Reducing BCs turn around time (TAT) could impact on multiple outcome parameters and TAT monitoring is an important tool for measurement of microbiology laboratory performance. The aim of this study was to provide an overview of BC TATs among Italian microbiology laboratories. <br /><em>Materials and methods</em>: Five laboratories collected and recorded, for a month period, date and time of the BC processing events. Cumulative TATs were analysed using the GraphPad software. <br /><em>Results</em>: Participating laboratories reported data from 302 sepsis episodes. The median time from when the BC system produced a positive signal until Gram-stain results were reported was 7.6 hours. A rapid molecular identification and antimicrobial susceptibility testing (AST) was performed in 26.5% of BCs. Mean TAT for identification report was significantly lower when a molecular approach was adopted (12 vs. 28.7 hours, P&lt;0.001). Similarly, results of the molecular AST were obtained more than 24 hours in advance compared with phenotypic AST (mean 13.2 vs. 47.6, P&lt;0.001). TATs from BC positivity of laboratories opened 7 days/week were not significantly lower than those of laboratories opened 6 days/week. <br /><em>Conclusions</em>: BC is a time-consuming exam, however, molecular identification and AST methods can drastically reduce time to results. The lack of difference between TATs observed for laboratories working 7 days/week and 6 days/week, coupled with a high rate of BCs turning positive during the night enable to conclude that the most urgent measure to reduce TATs is the expansion of laboratory regular duty hours.

scholarly journals Molecular Identification and Amphotericin B Susceptibility Testing of Clinical Isolates of Aspergillus From 11 Hospitals in Korea

2015 ◽  
Vol 35 (6) ◽  
pp. 602-610 ◽  
Author(s):  
Min Seok Heo ◽  
Jong Hee Shin ◽  
Min Ji Choi ◽  
Yeon-Joon Park ◽  
Hye Soo Lee ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Molecular Identification ◽  
Susceptibility Testing ◽  
Clinical Isolates

scholarly journals Molecular Identification and Susceptibility Pattern of ClinicalNocardiaSpecies: Emergence ofNocardia crassostreaeas an Agent of Invasive Nocardiosis

2013 ◽  
Vol 24 (2) ◽  
pp. e33-e38 ◽  
Author(s):  
Saad J Taj-Aldeen ◽  
Anand Deshmukh ◽  
Sanjay Doiphode ◽  
Atqah Abdul Wahab ◽  
Mona Allangawi ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Ribosomal Rna ◽  
Antimicrobial Agents ◽  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
16S Ribosomal Rna ◽  
Resistant Organism ◽  
Human Pathogen ◽  
Resistant Species ◽  
Nocardia Otitidiscaviarum

BACKGROUND:Nocardiaspecies are rare, opportunistic organisms that cause disease in both immunocompetent and immunocompromised individuals.OBJECTIVE: To investigate the clinical presentations of variousNocardiainfections based on the 16S ribosomal RNA gene of the isolate, as well as related risk factors and susceptibility patterns to antimicrobial agentsMETHODS: Thirteen patients with a diagnosis of nocardiosis were included in the present study. SevenNocardiaspecies were identified by 16S ribosomal RNA. Susceptibility testing was performed using six antimicrobial agents.RESULTS: Five patients were immunocompromised, and eight were immunocompetent with predisposing factors including cystic fibrosis, tuberculosis and ophthalmic infections.Nocardiacaused pulmonary infections in eight patients (61.5%), invasive systemic infections in three patients (23%) and local (ophthalmic) infections in two patients (15.4%). In the patients with pulmonary disease, nocardiosis was caused by six species (Nocardia cyriacigeorgica,Nocardia otitidiscaviarum,Nocardia farcinica,Nocardia carnea,Nocardia testaceaandNocardia asiatica). The seventh species identified in the present study wasNocardia crassostreae.DISCUSSION:N crassostreaeis a multidrug-resistant organism that was reported to be an emerging human pathogen causing invasive nocardiosis in a patient with non-Hodgkin’s lymphoma.N farcinicawas isolated from blood in a patient with breast cancer. None of theNocardiaisolates were resistant to linezolid. OneN otitidiscaviarumisolate was a multidrug-resistant organism. All patients in the present study were treated with the appropriate antibiotics and their condition resolved without further sequelae.CONCLUSIONS: The present study is the first report onN crassostreaeas a human pathogen. The detection of multidrug-resistant species necessitate molecular identification and susceptibility testing, and should be performed for allNocardiainfections. Nocardiosis manifests various clinical features depending on theNocardiaspecies and underlying conditions.

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