From slow to fast: effects of growth rate on global gene expression and recombinant protein secretion in Pichia pastoris

2014 ◽  
Vol 31 ◽  
pp. S6
Author(s):  
Corinna Rebnegger ◽  
Alexandra B. Graf ◽  
Minoska Valli ◽  
Brigitte Gasser ◽  
Michael Maurer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Rate ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Secretion ◽  
Global Gene Expression ◽  
Recombinant Protein Secretion

A New Signal Sequence for Recombinant Protein Secretion in Pichia pastoris

2014 ◽  
Vol 24 (3) ◽  
pp. 337-345 ◽  
Author(s):  
Nagaraj Govindappa ◽  
Manjunatha Hanumanthappa ◽  
Krishna Venkatarangaiah ◽  
Sankar Periyasamy ◽  
Suma Sreenivas ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Secretion ◽  
Signal Sequence ◽  
Recombinant Protein Secretion

Isolation of Pichia pastoris PIR genes and their utilization for cell surface display and recombinant protein secretion

Yeast ◽  
2010 ◽  
Vol 28 (3) ◽  
pp. 213-226 ◽  
Author(s):  
Yogender Pal Khasa ◽  
Sara Conrad ◽  
Mustafa Sengul ◽  
Sarah Plautz ◽  
Michael M. Meagher ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Cell Surface ◽  
Protein Secretion ◽  
Surface Display ◽  
Cell Surface Display ◽  
Recombinant Protein Secretion

scholarly journals Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

2014 ◽  
Vol 13 (1) ◽  
pp. 120
Author(s):  
Claudia Ruth ◽  
Markus Buchetics ◽  
Viktorija Vidimce ◽  
Daniela Kotz ◽  
Stefan Naschberger ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Secretion ◽  
Recombinant Protein Secretion

scholarly journals Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Claudia Ruth ◽  
Markus Buchetics ◽  
Viktorija Vidimce ◽  
Daniela Kotz ◽  
Stefan Naschberger ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Secretion ◽  
Recombinant Protein Secretion

Cross-species comparison of recombinant protein secretion in CHO cells and Pichia pastoris

2014 ◽  
Vol 31 ◽  
pp. S4
Author(s):  
Nils Landes ◽  
Andreas Maccani ◽  
Christian Leitner ◽  
Michael Maurer ◽  
Alexandra B. Graf ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Secretion ◽  
Cho Cells ◽  
Species Comparison ◽  
Recombinant Protein Secretion ◽  
Cross Species Comparison

scholarly journals The impact of ERAD on recombinant protein secretion in Pichia pastoris (syn Komagataella spp.)

Microbiology ◽  
2018 ◽  
Vol 164 (4) ◽  
pp. 453-463 ◽  
Author(s):  
Richard J. Zahrl ◽  
Diethard Mattanovich ◽  
Brigitte Gasser
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Protein Secretion ◽  
Recombinant Protein Secretion ◽  
The Impact

scholarly journals Transcriptome Analysis of Recombinant Protein Secretion by Aspergillus nidulans and the Unfolded-Protein Response In Vivo

2005 ◽  
Vol 71 (5) ◽  
pp. 2737-2747 ◽  
Author(s):  
Andrew H. Sims ◽  
Manda E. Gent ◽  
Karin Lanthaler ◽  
Nigel S. Dunn-Coleman ◽  
Stephen G. Oliver ◽  
...  
Keyword(s):  
Gene Expression ◽  
Recombinant Protein ◽  
Unfolded Protein Response ◽  
Aspergillus Nidulans ◽  
Filamentous Fungi ◽  
Protein Secretion ◽  
Unfolded Protein ◽  
The Unfolded Protein Response ◽  
Protein Response

ABSTRACT Filamentous fungi have a high capacity for producing large amounts of secreted proteins, a property that has been exploited for commercial production of recombinant proteins. However, the secretory pathway, which is key to the production of extracellular proteins, is rather poorly characterized in filamentous fungi compared to yeast. We report the effects of recombinant protein secretion on gene expression levels in Aspergillus nidulans by directly comparing a bovine chymosin-producing strain with its parental wild-type strain in continuous culture by using expressed sequence tag microarrays. This approach demonstrated more subtle and specific changes in gene expression than those observed when mimicking the effects of protein overproduction by using a secretion blocker. The impact of overexpressing a secreted recombinant protein more closely resembles the unfolded-protein response in vivo.

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