A novel approach employing ultrasound guidance for percutaneous cardiac muscle injection to retrograde label rat stellate ganglion neurons

2004 ◽  
Vol 363 (3) ◽  
pp. 252-256 ◽  
Author(s):  
Brad C. Fuller ◽  
Andrew D. Sumner ◽  
Michelle A. Kutzler ◽  
Victor Ruiz-Velasco
Respiration ◽  
2003 ◽  
Vol 70 (3) ◽  
pp. 299-301 ◽  
Author(s):  
Craig P. Hersh ◽  
David Feller-Kopman ◽  
Momen Wahidi ◽  
Robert Garland ◽  
Felix Herth ◽  
...  

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Harsha Shanthanna

We present this report of a young patient with chronic severe atypical facial pain who was successfully controlled with stellate ganglion block under ultrasound guidance. The patient had a history of severe disabling, unilateral, facial neuropathic pain with minimal response to analgesic medications. Upon assessment the patient had features suggestive of trigeminal neuralgia, although postherpetic neuralgia could not be ruled out. As a diagnostic test intervention, stellate ganglion block was tried under ultrasound guidance. The patient showed significant improvement in pain control and functional disability lasting beyond 10 weeks. Subsequent blocks reinforced the analgesia. Atypical facial pain has several differential diagnoses. The involvement of sympathetic system in its causation or sustenance is uncertain. Stellate ganglion block achieves sympathetic block of cervicofacial structures, and its blockade has been shown to affect chronic pain conditions. Although its mechanism is not clear, one has to consider its possible role in conditions of stress apart from directly controlling the sympathetic activity. There is certainly a role in exploring the potential benefits of stellate ganglion block in such clinical conditions. The technique of stellate block under ultrasound is also described, as it influences the safety and precision of the block.


2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Gaia Calamera ◽  
Dan Li ◽  
Andrea Hembre Ulsund ◽  
Jeong Joo Kim ◽  
Oliver C. Neely ◽  
...  

Abstract Several FRET (fluorescence resonance energy transfer)-based biosensors for intracellular detection of cyclic nucleotides have been designed in the past decade. However, few such biosensors are available for cGMP, and even fewer that detect low nanomolar cGMP concentrations. Our aim was to develop a FRET-based cGMP biosensor with high affinity for cGMP as a tool for intracellular signaling studies. We used the carboxyl-terminal cyclic nucleotide binding domain of Plasmodium falciparum cGMP-dependent protein kinase (PKG) flanked by different FRET pairs to generate two cGMP biosensors (Yellow PfPKG and Red PfPKG). Here, we report that these cGMP biosensors display high affinity for cGMP (EC50 of 23 ± 3 nM) and detect cGMP produced through soluble guanylyl cyclase and guanylyl cyclase A in stellate ganglion neurons and guanylyl cyclase B in cardiomyocytes. These biosensors are therefore optimal tools for real-time measurements of low concentrations of cGMP in living cells.


2020 ◽  
Vol 47 (12) ◽  
pp. 1932-1942
Author(s):  
Lijun Cheng ◽  
Huaying Fu ◽  
Xinghua Wang ◽  
Lan Ye ◽  
Ishan Lakhani ◽  
...  

2009 ◽  
Vol 87 (6) ◽  
pp. 1334-1342 ◽  
Author(s):  
Renato Albuquerque de Oliveira Cavalcanti ◽  
Demilto Yamaguchi da Pureza ◽  
Mariana Pereira de Melo ◽  
Romeu Rodrigues de Souza ◽  
Cássia T. Bergamaschi ◽  
...  

2008 ◽  
Vol 75 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Chunping Zhang ◽  
Guilin Li ◽  
Shangdong Liang ◽  
Changshui Xu ◽  
Gaochun Zhu ◽  
...  

2005 ◽  
Vol 15 (4) ◽  
pp. 671-675 ◽  
Author(s):  
K. Sieunarine ◽  
A. S. Cowie ◽  
J. D. Bartlett ◽  
I. Lindsay ◽  
J. R. Smith

Adenomatoid tumors of the uterus are uncommon benign lesions derived from mesothelium, with a prevalence of 1.2% in one study of 1 000 unselected hysterectomy specimens. They are usually small and near the serosal surface; however, they may be large and diffuse (giant adenomatoid tumors). They coexist with leiomyomas in 60% of cases. A 33-year-old nulliparous woman was referred for severe menorrhagia and dysmenorrhea, thought to be due to a submucosal fibroid on ultrasound. This transpired to be an adenomatoid tumor, and she underwent three transcervical resections of the tumor (TCRT) over a period of 12 months for tumor recurrence and failure of symptom resolution. The last TCRT was performed with ultrasound guidance and laparoscopic visualization of the uterus to the resection point of blanching of the serosal surface. She failed to respond to a GnRH analogue throughout. A specialist opinion on the suitability of vascular embolization of the tumor judged that it would be ineffective for this lesion. She then underwent a Strassman procedure and removal of the adenomatoid tumor. This involved dissection of ureters and pelvic vasculature, selective temporary ligation of uterine arteries, hemisection of the uterus, and excision of the tumor with frozen sections to ensure clear tumor margins and resuturing of the uterine halves. Temporary vascular occlusion of the uterine arteries and ovarian vessels allowed a Strassman procedure, which resulted in successful resection of a recurrent giant adenomatoid tumor of the uterus, with fertility preservation in a young nulliparous woman. Two and a half years on there is no evidence of tumor recurrence.


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