Involvement of equilibrative and concentrative nucleoside transporters in hydrogen peroxide-induced thymidine incorporation into cultured astrocytes

2009 ◽  
Vol 65 ◽  
pp. S148
Author(s):  
Koh-ichi Tanaka ◽  
Junichi Kitanaka ◽  
Nobue Kitanaka ◽  
Tomoaki Sato ◽  
Takashige Nishikawa ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Thymidine Incorporation ◽  
Nucleoside Transporters ◽  
Cultured Astrocytes

scholarly journals Expression of nucleoside transporters and hydrogen peroxide-induced thymidine incorporation in astrocytes

2020 ◽  
Vol 93 (0) ◽  
pp. 3-P-277
Author(s):  
Koh-ichi Tanaka ◽  
Kento Igarashi ◽  
Kazuo Tomita ◽  
Nobue Kitanaka ◽  
Junichi Kita ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Thymidine Incorporation ◽  
Nucleoside Transporters

scholarly journals Oxidative Stress Posttranslationally Regulates the Expression of Ha-Ras and Ki-Ras in Cultured Astrocytes

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Samantha Messina ◽  
Luigi Frati ◽  
Antonio Porcellini
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Map Kinase ◽  
Protein Synthesis Inhibitor ◽  
Synthesis Inhibitor ◽  
Cultured Astrocytes ◽  
Map Kinase Pathway ◽  
Safe Mechanism ◽  
Fail Safe ◽  
Kinase Pathway

Addition of hydrogen peroxide to cultured astrocytes induced a rapid and transient increase in the expression of Ha-Ras and Ki-Ras. Pull-down experiments with the GTP-Ras-binding domain of Raf-1 showed that oxidative stress substantially increased the activation of Ha-Ras, whereas a putative farnesylated activated form of Ki-Ras was only slightly increased. The increase in both Ha-Ras and Ki-Ras was insensitive to the protein synthesis inhibitor, cycloheximide, and was occluded by the proteasomal inhibitor, MG-132. In addition, exposure to hydrogen peroxide reduced the levels of ubiquitinated Ras protein, indicating that oxidative stress leads to a reduced degradation of both isoforms through the ubiquitin/proteasome pathway. Indeed, the late reduction in Ha-Ras and Ki-Ras was due to a recovery of proteasomal degradation because it was sensitive to MG-132. The late reduction of Ha-Ras levels was abrogated by compound PD98059, which inhibits the MAP kinase pathway, whereas the late reduction of Ki-Ras was unaffected by PD98059. We conclude that oxidative stress differentially regulates the expression of Ha-Ras and Ki-Ras in cultured astrocytes, and that activation of the MAP kinase pathway by oxidative stress itself or by additional factors may act as a fail-safe mechanism limiting a sustained expression of the potentially detrimental Ha-Ras.

scholarly journals Induction of heat shock proteins (HSPs) by sodium arsenite in cultured astrocytes and reduction of hydrogen peroxide-induced cell death

2002 ◽  
Vol 83 (6) ◽  
pp. 1338-1348 ◽  
Author(s):  
Bernard Fauconneau ◽  
Valérie Petegnief ◽  
Coral Sanfeliu ◽  
Alain Piriou ◽  
Anna M. Planas
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Death ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Sodium Arsenite ◽  
Cultured Astrocytes ◽  
Shock Proteins

The aim of thymidine incorporation via nucleoside transporters on oxidative stress

2011 ◽  
Vol 71 ◽  
pp. e222
Author(s):  
Koh-ichi Tanaka ◽  
Nobue Kitanaka ◽  
Junichi Kitanaka ◽  
Yuriko Kishi ◽  
Shyohn Yokoyama ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thymidine Incorporation ◽  
Nucleoside Transporters

scholarly journals Hydrogen peroxide-induced thymidine incorporation into cultured rat cerebral astrocytes

1998 ◽  
Vol 76 ◽  
pp. 55
Author(s):  
Koh-ichi Tanaka ◽  
Yoshiko Ohnishi ◽  
Tomoaki Sato ◽  
Takashige Nishikawa
Keyword(s):  
Hydrogen Peroxide ◽  
Thymidine Incorporation

Heat shock inhibits hydrogen peroxide-induced apoptosis in cultured astrocytes

2002 ◽  
Vol 946 (2) ◽  
pp. 232-238 ◽  
Author(s):  
Kazuhiro Takuma ◽  
Koichi Mori ◽  
Eibai Lee ◽  
Riyo Enomoto ◽  
Akemichi Baba ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Heat Shock ◽  
Cultured Astrocytes ◽  
Induced Apoptosis

scholarly journals Hydrogen Peroxide-Induced Thymidine Incorporation Into Cultured Rat Astrocytes

2006 ◽  
Vol 102 (3) ◽  
pp. 296-304 ◽  
Author(s):  
Koh-ichi Tanaka ◽  
Tomoaki Sato ◽  
Yoshiko Ohnishi ◽  
Takashige Nishikawa
Keyword(s):  
Hydrogen Peroxide ◽  
Thymidine Incorporation ◽  
Rat Astrocytes

Sustained hydrogen peroxide stress decreases lactate production by cultured astrocytes

2009 ◽  
Vol 87 (12) ◽  
pp. 2696-2708 ◽  
Author(s):  
Jeff R. Liddell ◽  
Claudia Zwingmann ◽  
Maike M. Schmidt ◽  
Anette Thiessen ◽  
Dieter Leibfritz ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lactate Production ◽  
Cultured Astrocytes ◽  
Peroxide Stress
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