Comparative effects of α-tocopherol and γ-tocotrienol against hydrogen peroxide induced apoptosis on primary-cultured astrocytes

2006 ◽  
Vol 243 (1-2) ◽  
pp. 5-12 ◽  
Author(s):  
Musalmah Mazlan ◽  
Sue-Mian Then ◽  
Gapor Mat Top ◽  
Wan Zurinah Wan Ngah
Keyword(s):  
Hydrogen Peroxide ◽  
Cultured Astrocytes ◽  
Induced Apoptosis ◽  
Primary Cultured Astrocytes

scholarly journals Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaodan Xu ◽  
Hong Zhang ◽  
Ke Wang ◽  
Tao Tu ◽  
Yuan Jiang
Keyword(s):  
Carbon Monoxide ◽  
Membrane Potential ◽  
Protective Effect ◽  
Caspase 3 ◽  
Prolonged Exposure ◽  
Apoptosis Pathway ◽  
Apoptosis Rate ◽  
Cultured Astrocytes ◽  
Induced Apoptosis ◽  
Primary Cultured Astrocytes

Objective. To observe the protective effect of edaravone (Eda) on astrocytes after prolonged exposure to carbon monoxide (CO) and further to investigate the potential mechanisms of Eda against CO-induced apoptosis. Methods. The rat primary cultured astrocytes were cultured in vitro and exposed to 1% CO for 24 h after being cultured with different concentrations of Eda. MTT assay was used to detect the cytotoxicity of CO. Flow cytometry was used to detect the apoptosis rate, membrane potential of mitochondria, and ROS level. The mRNA and protein expressions of Bcl-2, Bax, and caspase-3 were assessed by real-time PCR and Western blotting analysis, respectively. Results. Eda can significantly suppress cytotoxicity of CO, and it can significantly increase membrane potential of mitochondria and Bcl-2 expressions and significantly suppress the apoptosis rate, ROS level, Bax, and caspase-3 expressions. Conclusion. Eda protects against CO-induced apoptosis in rat primary cultured astrocytes through decreasing ROS production and subsequently inhibiting mitochondrial apoptosis pathway.

Heat shock inhibits hydrogen peroxide-induced apoptosis in cultured astrocytes

2002 ◽  
Vol 946 (2) ◽  
pp. 232-238 ◽  
Author(s):  
Kazuhiro Takuma ◽  
Koichi Mori ◽  
Eibai Lee ◽  
Riyo Enomoto ◽  
Akemichi Baba ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Heat Shock ◽  
Cultured Astrocytes ◽  
Induced Apoptosis

scholarly journals Anethole prevents hydrogen peroxide-induced apoptosis and collagen metabolism alterations in human skin fibroblasts

2014 ◽  
Vol 394 (1-2) ◽  
pp. 217-224 ◽  
Author(s):  
Anna Galicka ◽  
Rafał Krętowski ◽  
Jolanta Nazaruk ◽  
Marzanna Cechowska-Pasko
Keyword(s):  
Hydrogen Peroxide ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Collagen Metabolism ◽  
Human Skin Fibroblasts ◽  
Induced Apoptosis
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