scholarly journals Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development

Neuron ◽  
2019 ◽  
Vol 103 (5) ◽  
pp. 836-852.e5 ◽  
Author(s):  
Naoki Nakagawa ◽  
Charlotte Plestant ◽  
Keiko Yabuno-Nakagawa ◽  
Jingjun Li ◽  
Janice Lee ◽  
...  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mariko Umemura ◽  
Yasuyuki Kaneko ◽  
Ryoko Tanabe ◽  
Yuji Takahashi

AbstractActivating transcription factor 5 (ATF5) is a member of the cAMP response element binding protein (CREB)/ATF family of basic leucine zipper transcription factors. We previously reported that ATF5-deficient (ATF5−/−) mice exhibited behavioural abnormalities, including abnormal social interactions, reduced behavioural flexibility, increased anxiety-like behaviours, and hyperactivity in novel environments. ATF5−/− mice may therefore be a useful animal model for psychiatric disorders. ATF5 is highly expressed in the ventricular zone and subventricular zone during cortical development, but its physiological role in higher-order brain structures remains unknown. To investigate the cause of abnormal behaviours exhibited by ATF5−/− mice, we analysed the embryonic cerebral cortex of ATF5−/− mice. The ATF5−/− embryonic cerebral cortex was slightly thinner and had reduced numbers of radial glial cells and neural progenitor cells, compared to a wild-type cerebral cortex. ATF5 deficiency also affected the basal processes of radial glial cells, which serve as a scaffold for radial migration during cortical development. Further, the radial migration of cortical upper layer neurons was impaired in ATF5−/− mice. These results suggest that ATF5 deficiency affects cortical development and radial migration, which may partly contribute to the observed abnormal behaviours.


eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Kerstin Hasenpusch-Theil ◽  
Christine Laclef ◽  
Matt Colligan ◽  
Eamon Fitzgerald ◽  
Katherine Howe ◽  
...  

During the development of the cerebral cortex, neurons are generated directly from radial glial cells or indirectly via basal progenitors. The balance between these division modes determines the number and types of neurons formed in the cortex thereby affecting cortical functioning. Here, we investigate the role of primary cilia in controlling the decision between forming neurons directly or indirectly. We show that a mutation in the ciliary gene Inpp5e leads to a transient increase in direct neurogenesis and subsequently to an overproduction of layer V neurons in newborn mice. Loss of Inpp5e also affects ciliary structure coinciding with reduced Gli3 repressor levels. Genetically restoring Gli3 repressor rescues the decreased indirect neurogenesis in Inpp5e mutants. Overall, our analyses reveal how primary cilia determine neuronal subtype composition of the cortex by controlling direct versus indirect neurogenesis. These findings have implications for understanding cortical malformations in ciliopathies with INPP5E mutations.


2008 ◽  
pp. 159-169
Author(s):  
Eckart Förster ◽  
Shanting Zhao ◽  
Michael Frotscher

Sign in / Sign up

Export Citation Format

Share Document