P047. Conventional screening method of short term in vitro assay for nitric oxide scavengers

Nitric Oxide ◽  
2006 ◽  
Vol 14 (4) ◽  
pp. 32-33
Author(s):  
Harukuni Tokuda ◽  
T. Akihisa ◽  
R. Tanaka ◽  
J. Takayasu ◽  
E. Fumio ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Screening Method ◽  
In Vitro Assay ◽  
Short Term ◽  
Vitro Assay

P012. Conventional screening method of short-term in vitro assay for nitric oxide scavenger reagents

Nitric Oxide ◽  
2006 ◽  
Vol 14 (4) ◽  
pp. 20
Author(s):  
Harukuni Tokuda ◽  
Toshihiro Akihisa ◽  
Reiko Tanaka ◽  
Junko Takayasu ◽  
Hoyoku Nishino
Keyword(s):  
Nitric Oxide ◽  
Screening Method ◽  
In Vitro Assay ◽  
Short Term ◽  
Vitro Assay

scholarly journals Dietary Preference of Newly Weaned Pigs and Nutrient Interactions According to Copper Levels and Sources with Different Solubility Characteristics

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1133 ◽  
Author(s):  
Sandra Villagómez-Estrada ◽  
José Francisco Pérez ◽  
Sandra van Kuijk ◽  
Diego Melo-Durán ◽  
Razzagh Karimirad ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Feeding Preference ◽  
In Vitro Assay ◽  
Nutrient Interactions ◽  
Short Term ◽  
Vitro Assay ◽  
Dietary Preference ◽  
Weaning Pigs ◽  
Weaned Pig

Two feeding preference experiments and an in vitro assay were performed to assess the weaned pig preference for Cu doses and sources based on their sensorial perception and on the likely post-ingestive effects of Cu. At day 7 post-weaning, a total of 828 pigs were distributed into two different experiments. In Exp.1 (dose preference) a diet with a nutritional Cu level (15 mg/kg) of Cu sulfate (SF) was pair offered with higher Cu levels (150 mg/kg) of either SF or hydroxychloride (HCl). In Exp.2 (source preference), a diet supplemented with Cu-SF at 150 mg/kg was compared to a Cu-HCl (150 mg/kg) diet. At the short-term (day 7–9) and for the entire experimental week (day 7–14), pigs preferred diets with a high Cu level than with Cu at a nutritional dose (p < 0.05). Likewise, pigs preferred diets supplemented with a Cu-HCl source compared to diets with Cu-SF (p < 0.05). In vitro assay results showed a greater solubility and interaction of Cu-SF with phytic acid compared to Cu-HCl. In conclusion, pigs chose diets with higher levels of Cu probably to re-establish homeostasis after weaning. Pigs preferred diets with Cu-HCl compared to Cu-SF probably due to their solubilities and chemical differences.

scholarly journals Release from Quiescence of Primitive Human Hematopoietic Stem/Progenitor Cells by Blocking Their Cell-Surface TGF-β Type II Receptor in a Short-Term In Vitro Assay

Stem Cells ◽  
2000 ◽  
Vol 18 (2) ◽  
pp. 102-111 ◽  
Author(s):  
Nicolas Fortunel ◽  
Jacques Hatzfeld ◽  
Sergueï Kisselev ◽  
Marie-Noëlle Monier ◽  
Karin Ducos ◽  
...  
Keyword(s):  
Cell Surface ◽  
Progenitor Cells ◽  
In Vitro Assay ◽  
Type Ii ◽  
Short Term ◽  
Vitro Assay ◽  
Hematopoietic Stem

BEHAVIOURAL ACTION OF ANDROGEN IN THE DOVE: EFFECTS OF LONG-TERM CASTRATION ON RESPONSE SPECIFICITY AND BRAIN AROMATIZATION

1981 ◽  
Vol 90 (2) ◽  
pp. 167-178 ◽  
Author(s):  
J. B. HUTCHISON ◽  
TH. STEIMER ◽  
R. DUNCAN
Keyword(s):  
Preoptic Area ◽  
In Vitro Assay ◽  
Aromatase Activity ◽  
Male Courtship ◽  
Short Term ◽  
Vitro Assay ◽  
Vocal Behaviour ◽  
Response Specificity

Differences in the effectiveness of oestradiol-17β and testosterone on male courtship and vocal behaviour were examined in long-term castrated doves. Nest-orientated behaviour was restored by intramuscular injection of oestradiol-17β. Testosterone was effective in restoring aggressive courtship and vocal behaviour, but not for the nest-orientated behaviour. The effects of these hormones were separable, therefore, under conditions of prolonged androgen deficit, suggesting differences in their specificity of action. In-vitro assay of brain enzyme activity indicated that aromatization of testosterone to oestradiol-17β occurred in the preoptic area of long-term castrated doves. Preoptic aromatase activity of long- and short-term castrated doves did not differ. The ineffectiveness of testosterone in restoring nest-orientated behaviour in long-term castrated doves did not appear, therefore, to be due to a difference between the groups in the basal rate of testosterone aromatization in the preoptic area.

scholarly journals Establishment of a Short-Term In Vitro Assay for Mouse Spermatogonial Stem Cells1

2007 ◽  
Vol 77 (5) ◽  
pp. 897-904 ◽  
Author(s):  
Jonathan R. Yeh ◽  
Xiangfan Zhang ◽  
Makoto C. Nagano
Keyword(s):  
In Vitro Assay ◽  
Short Term ◽  
Vitro Assay

Inhibitory effect of iridoids on Epstein-Barr virus activation by a short-term in vitro assay for anti-tumor promoters

1996 ◽  
Vol 102 (1-2) ◽  
pp. 223-226 ◽  
Author(s):  
Govind J. Kapadia ◽  
Shekhar C. Sharma ◽  
Harukuni Tokuda ◽  
Hoyoku Nishino ◽  
Shinichi Ueda
Keyword(s):  
Epstein Barr Virus ◽  
Inhibitory Effect ◽  
In Vitro Assay ◽  
Tumor Promoters ◽  
Short Term ◽  
Vitro Assay ◽  
Barr Virus ◽  
Epstein Barr

A short-term in vitro assay for promoter substances using human lymphoblastoid cells latently infected with Epstein-Barr virus

1981 ◽  
Vol 13 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Yohei Ito ◽  
Sugio Yanase ◽  
Jun Fujita ◽  
Takashi Harayama ◽  
Matao Takashima ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
In Vitro Assay ◽  
Short Term ◽  
Lymphoblastoid Cells ◽  
Vitro Assay ◽  
Barr Virus ◽  
Latently Infected ◽  
Epstein Barr

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

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