P.19.8 The effect of water-soluble fullerene with different number of hydroxyl groups in muscle regeneration process of experimental murine skeletal muscle

2013 ◽  
Vol 23 (9-10) ◽  
pp. 838 ◽  
Author(s):  
A. Ishii ◽  
N. Ohkoshi ◽  
M. Yoshida ◽  
A. Tamaoka
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Water Soluble ◽  
Regeneration Process ◽  
Hydroxyl Groups ◽  
Effect Of Water ◽  
Murine Skeletal Muscle

scholarly journals Monocyte/Macrophage-derived IGF-1 Orchestrates Murine Skeletal Muscle Regeneration and Modulates Autocrine Polarization

2015 ◽  
Vol 23 (7) ◽  
pp. 1189-1200 ◽  
Author(s):  
Joanne Tonkin ◽  
Lieve Temmerman ◽  
Robert D Sampson ◽  
Enrique Gallego-Colon ◽  
Laura Barberi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Skeletal Muscle Regeneration ◽  
Murine Skeletal Muscle

scholarly journals Characterization of the Skeletal Muscle Secretome Reveals a Role for Extracellular Vesicles and IL1α/IL1β in Restricting Fibro/Adipogenic Progenitor Adipogenesis

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1171
Author(s):  
Simone Vumbaca ◽  
Giulio Giuliani ◽  
Valeria Fiorentini ◽  
Flavia Tortolici ◽  
Andrea Cerquone Perpetuini ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Fate ◽  
Extracellular Vesicles ◽  
Muscle Regeneration ◽  
Adipogenic Differentiation ◽  
Control Cell ◽  
Regeneration Process ◽  
Adult Skeletal Muscle ◽  
Pathological Conditions ◽  
Cell Cell

Repeated mechanical stress causes injuries in the adult skeletal muscle that need to be repaired. Although muscle regeneration is a highly efficient process, it fails in some pathological conditions, compromising tissue functionality. This may be caused by aberrant cell–cell communication, resulting in the deposition of fibrotic and adipose infiltrates. Here, we investigate in vivo changes in the profile of skeletal muscle secretome during the regeneration process to suggest new targetable regulatory circuits whose failure may lead to tissue degeneration in pathological conditions. We describe the kinetic variation of expression levels of 76 secreted proteins during the regeneration process. In addition, we profile the gene expression of immune cells, endothelial cells, satellite cells, and fibro-adipogenic progenitors. This analysis allowed us to annotate each cell-type with the cytokines and receptors they have the potential to synthetize, thus making it possible to draw a cell–cell interaction map. We next selected 12 cytokines whose receptors are expressed in FAPs and tested their ability to modulate FAP adipogenesis and proliferation. We observed that IL1α and IL1β potently inhibit FAP adipogenesis, while EGF and BTC notably promote FAP proliferation. In addition, we characterized the cross-talk mediated by extracellular vesicles (EVs). We first monitored the modulation of muscle EV cargo during tissue regeneration. Using a single-vesicle flow cytometry approach, we observed that EVs differentially affect the uptake of RNA and proteins into their lumen. We also investigated the EV capability to interact with SCs and FAPs and to modulate their proliferation and differentiation. We conclude that both cytokines and EVs secreted during muscle regeneration have the potential to modulate adipogenic differentiation of FAPs. The results of our approach provide a system-wide picture of mechanisms that control cell fate during the regeneration process in the muscle niche.

scholarly journals TAM kinase signaling is indispensable for proper skeletal muscle regeneration in mice

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Nour Al-Zaeed ◽  
Zsófia Budai ◽  
Zsuzsa Szondy ◽  
Zsolt Sarang
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Kinase Inhibitor ◽  
Skeletal Muscle Regeneration ◽  
Regeneration Process ◽  
Phenotypic Change ◽  
C2c12 Myoblast ◽  
Kinase Signaling ◽  
Cell Debris ◽  
Myogenic Stem Cells

AbstractSkeletal muscle regeneration following injury results from the proliferation and differentiation of myogenic stem cells, called satellite cells, located beneath the basal lamina of the muscle fibers. Infiltrating macrophages play an essential role in the process partly by clearing the necrotic cell debris, partly by producing cytokines that guide myogenesis. Infiltrating macrophages are at the beginning pro-inflammatory, but phagocytosis of dead cells induces a phenotypic change to become healing macrophages that regulate inflammation, myoblast fusion and growth, fibrosis, vascularization and return to homeostasis. The TAM receptor kinases Mer and Axl are known efferocytosis receptors in macrophages functioning in tolerogenic or inflammatory conditions, respectively. Here we investigated their involvement in the muscle regeneration process by studying the muscle repair following cardiotoxin-induced injury in Mer−/− mice. We found that Axl was the only TAM kinase receptor expressed on the protein level by skeletal muscle and C2C12 myoblast cells, while Mer was the dominant TAM kinase receptor in the CD45+ cells, and its expression significantly increased during repair. Mer ablation did not affect the skeletal muscle weight or structure, but following injury it resulted in a delay in the clearance of necrotic muscle cell debris, in the healing phenotype conversion of macrophages and consequently in a significant delay in the full muscle regeneration. Administration of the TAM kinase inhibitor BMS-777607 to wild type mice mimicked the effect of Mer ablation on the muscle regeneration process, but in addition, it resulted in a long-persisting necrotic area. Finally, in vitro inhibition of TAM kinase signaling in C2C12 myoblasts resulted in decreased viability and in impaired myotube growth. Our work identifies Axl as a survival and growth receptor in the mouse myoblasts, and reveals the contribution of TAM kinase-mediated signaling to the skeletal muscle regeneration both in macrophages and in myoblasts.

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