scholarly journals Cell therapy with human induced pluripotent stem cell-derived retinal pigment epithelium and retinal precursor cells prevents visual function loss in a rat model of retinal degeneration

2021 ◽  
Author(s):  
Anna Salas ◽  
Anna Duarri ◽  
Laura Fontrodona ◽  
Diana Mora Ramírez ◽  
Anna Badia ◽  
...  
Keyword(s):  
Stem Cell ◽  
Retinal Pigment Epithelium ◽  
Cell Therapy ◽  
Rat Model ◽  
Pluripotent Stem Cell ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Induced Pluripotent Stem Cell ◽  
Function Loss ◽  
Induced Pluripotent

scholarly journals Soy Protein Nanofiber Scaffolds for Uniform Maturation of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

2020 ◽  
Vol 26 (8) ◽  
pp. 433-446
Author(s):  
Michael A. Phelan ◽  
Kamil Kruczek ◽  
John H. Wilson ◽  
Matthew J. Brooks ◽  
Charles T. Drinnan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Retinal Pigment Epithelium ◽  
Soy Protein ◽  
Pluripotent Stem Cell ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Induced Pluripotent Stem Cell ◽  
Nanofiber Scaffolds ◽  
Induced Pluripotent

Subretinal Transplant of Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium on Nanostructured Fibrin-Agarose

2019 ◽  
Vol 25 (9-10) ◽  
pp. 799-808 ◽  
Author(s):  
Ana B. García Delgado ◽  
Berta de la Cerda ◽  
Julia Alba Amador ◽  
Maria Lourdes Valdés Sánchez ◽  
Beatriz Fernández-Muñoz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Retinal Pigment Epithelium ◽  
Pluripotent Stem Cell ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

scholarly journals Transplantation of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium in a Swine Model of Geographic Atrophy

2021 ◽  
Vol 22 (19) ◽  
pp. 10497
Author(s):  
Anna Duarri ◽  
Eduardo Rodríguez-Bocanegra ◽  
Gema Martínez-Navarrete ◽  
Marc Biarnés ◽  
Miriam García ◽  
...  
Keyword(s):  
Stem Cell ◽  
Retinal Pigment Epithelium ◽  
Pluripotent Stem Cell ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Induced Pluripotent Stem Cell ◽  
Geographic Atrophy ◽  
Swine Model ◽  
Rpe Cells ◽  
Induced Pluripotent

Background: The aim of this study was to test the feasibility and safety of subretinal transplantation of human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (RPE) cells into the healthy margins and within areas of degenerative retina in a swine model of geographic atrophy (GA). Methods: Well-delimited selective outer retinal damage was induced by subretinal injection of NaIO3 into one eye in minipigs (n = 10). Thirty days later, a suspension of hiPSC-derived RPE cells expressing green fluorescent protein was injected into the subretinal space, into the healthy margins, and within areas of degenerative retina. In vivo follow-up was performed by multimodal imaging. Post-mortem retinas were analyzed by immunohistochemistry and histology. Results: In vitro differentiated hiPSC-RPE cells showed a typical epithelial morphology, expressed RPE-related genes, and had phagocytic ability. Engrafted hiPSC-RPE cells were detected in 60% of the eyes, forming mature epithelium in healthy retina extending towards the border of the atrophy. Histological analysis revealed RPE interaction with host photoreceptors in the healthy retina. Engrafted cells in the atrophic zone were found in a patchy distribution but failed to form an epithelial-like layer. Conclusions: These results might support the use of hiPSC-RPE cells to treat atrophic GA by providing a housekeeping function to aid the overwhelmed remnant RPE, which might improve its survival and therefore slow down the progression of GA.

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