scholarly journals Species misidentification in local markets: Discrepancies between reporting and molecular identification of bushmeat species in northern Uganda

One Health ◽  
2021 ◽  
Vol 13 ◽  
pp. 100251
Author(s):  
BreeAnna Dell ◽  
Charles Masembe ◽  
Richard Gerhold ◽  
Adam Willcox ◽  
Chika Okafor ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Northern Uganda ◽  
Local Markets

scholarly journals Molecular Identification of Shark Meat From Local Markets in Southern Brazil Based on DNA Barcoding: Evidence for Mislabeling and Trade of Endangered Species

2018 ◽  
Vol 9 ◽  
Author(s):  
Fernanda Almerón-Souza ◽  
Christian Sperb ◽  
Carolina L. Castilho ◽  
Pedro I. C. C. Figueiredo ◽  
Leonardo T. Gonçalves ◽  
...  
Keyword(s):  
Endangered Species ◽  
Dna Barcoding ◽  
Molecular Identification ◽  
Southern Brazil ◽  
Local Markets

scholarly journals Molecular Identification of Onchocerca spp. Larvae in Simulium damnosum sensu lato Collected in Northern Uganda

2017 ◽  
Author(s):  
Guilherme G. Verocai ◽  
Philbert Clouds ◽  
Thomson Lakwo ◽  
James Katamanywa ◽  
Thomas R. Unnasch ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Northern Uganda ◽  
Simulium Damnosum

scholarly journals Molecular identification of shark fins in Malaysian Borneo’s local markets

2018 ◽  
Vol 19 (3) ◽  
pp. 1035-1043 ◽  
Author(s):  
BADRUL MUNIR MD-ZAIN ◽  
SITI NUR AZMINA ABID-KAMAL ◽  
NOR RAHMAN AIFAT ◽  
MUHAMMAD ABU BAKAR ABDUL-LATIFF ◽  
ABU MOHD- HASHIM ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Dna Sequences ◽  
Molecular Study ◽  
Genetic Identification ◽  
Coi Gene ◽  
Local Markets ◽  
Sphyrna Lewini ◽  
Phylogeny Analysis ◽  
Tree Topologies ◽  
Malaysian Borneo

Abstract. Md-Zain BM, Abid-Kamal SNA, Aifat NR, Abdul-Latiff MAB, Mohd-Hashim A, Ampeng A, Yaakop S, Samat A. 2018.Molecular identification of shark fins in Malaysian Borneo’s local markets. Biodiversitas 19: 1035-1043. A molecular study was carriedout to investigate the potential of the cytochrome c oxidase subunit I (COI) gene as a molecular marker for the genetic identification ofshark fin samples that have gone through various preservation processes. A total number of 17 shark fin samples were collected fromlocal markets in Sabah and Sarawak (Malaysian Borneo). The DNA sequences of the 17 samples were amplified by using polymerasechain reaction. The results from the analysis showed that, in the 17 sequences, there were 16 haplotypes present, with 244 sites from 688bp of the sequences. For phylogeny analysis, tree topologies were reconstructed using the neighbor-joining (NJ) and maximumparsimony (MP) methods. DNA barcoding technique successfully identifies shark fins collected in local markets in Malaysian Borneo atspecies level employed during this study. Phylogenetic analysis showed that there were four clades that distinguish the four differentorders present in the sample species. These clades had bootstrap values higher than 80. In addition, results indicated that 88.2% of theindividuals are listed as endangered (Lamiopsis tephrodes, Sphyrna mokarran, and Sphyrna lewini), vulnerable (Alopias pelagicus andRhynchobatus australiae), and near threatened (Carcharhinus limbatus, Chiloscyllium griseum, Carcharhinus sorrah, and Carcharhinusbrevipinna), in the International Union for Conservation of Nature (IUCN) Red Data List.

scholarly journals Molecular Identification of Onchocerca spp. Larvae in Simulium damnosum sensu lato Collected in Northern Uganda

2017 ◽  
Vol 97 (6) ◽  
pp. 1843-1845
Author(s):  
Guilherme G. Verocai ◽  
Hassan K. Hassan ◽  
Thomson Lakwo ◽  
Peace Habomugisha ◽  
Moses N. Katabarwa ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Northern Uganda ◽  
Simulium Damnosum

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

Isolation and Diagnosis of Fungi from Samples of Spices in the City of Nasiriya

2020 ◽  
pp. 7-9
Keyword(s):  
Chronic Diseases ◽  
Dilution Method ◽  
Local Markets ◽  
White Pepper ◽  
Black And White ◽  
Different Types ◽  
Potential Risks ◽  
The City ◽  
Isolated Species

Examination of (35) samples of spices obtained from local markets for the purposes of isolating and diagnosing fungi growing on them. Anine isolates belonging to 13 different types of fungi were diagnosed by the standard dilution method with three replications, and it has been observed that the most samples from which the fungi were isolated is ginger. It was found that the most isolated species of fungi are Penicillium, Aspergillus, and Rizupes spp. A rare colony of fungi was observed, which indicates contamination of the spices under study with the fungus. The present study aims to identify the potential risks of the presence of fungi in spices and what may result from mycotoxins that may be the cause of many chronic diseases as a result of using these spices in large quantities. The study recommends limiting the use of contaminated spices, especially ginger, in preparing food and its uses, in addition to other types such as cloves, black and white pepper, and other types of spices found in the local markets, especially the expired ones.

Sign in / Sign up

Export Citation Format

Share Document