Molecular detection and genetic diversity of Babesia canis canis in pet dogs in Henan Province, China

2019 ◽  
Vol 71 ◽  
pp. 37-40 ◽  
Author(s):  
Jinming Wang ◽  
Junlong Liu ◽  
Jifei Yang ◽  
Zhijie Liu ◽  
Xiaoxing Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Detection ◽  
Henan Province ◽  
Babesia Canis ◽  
Pet Dogs ◽  
Babesia Canis Canis

Molecular detection of Babesia canis in Dermacentor reticulatus ticks collected in Slovakia

Biologia ◽  
2006 ◽  
Vol 61 (2) ◽  
Author(s):  
Darja Duh ◽  
Mirko Slovák ◽  
Ana Saksida ◽  
Katja Strašek ◽  
Miroslav Petrovec ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Vector Competence ◽  
Dermacentor Reticulatus ◽  
Molecular Evidence ◽  
Babesia Canis ◽  
Aetiological Agent ◽  
Canine Babesiosis ◽  
Babesia Canis Canis

AbstractDermacentor reticulatus ticks are recognized as the most important vectors of Babesia canis, the aetiological agent of canine babesiosis occurring throughout Europe. Vector competence of D. reticulatus for B. canis is well described and experimentally determined; however, by using molecular analysis it was proven so by one recent study in Russia. Herein, the additional molecular evidence of B. canis infection in D. reticulatus ticks collected in Slovakia is provided. Using PCR followed by sequencing of distinctive amplicons we determined the presence of Babesia canis canis in one of 100 tested adult ticks. Two zoonotic pathogens, Francisella tularensis and Coxiella burnetii, were previously isolated from D. reticulatus ticks in Slovakia. In our samples, we detected only the presence of F. tularensis.

Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs

2002 ◽  
Vol 106 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Simone M. Cacciò ◽  
Boris Antunovic ◽  
Annabella Moretti ◽  
Vittorio Mangili ◽  
Albert Marinculic ◽  
...  
Keyword(s):  
Babesia Canis ◽  
Molecular Characterisation ◽  
Babesia Canis Canis

scholarly journals Molecular Detection and Genetic Diversity of Casuarina Moth, Lymantria xylina (Lepidoptera: Erebidae)

2018 ◽  
Vol 18 (3) ◽  
Author(s):  
Rong Wang ◽  
Zhihan Zhang ◽  
Xia Hu ◽  
Songqing Wu ◽  
Jinda Wang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Detection

The prevention of transmission of Babesia canis canis by Dermacentor reticulatus ticks to dogs using a novel combination of fipronil, amitraz and (S)-methoprene

2011 ◽  
Vol 179 (4) ◽  
pp. 343-350 ◽  
Author(s):  
Frans Jongejan ◽  
Josephus J. Fourie ◽  
S. Theodore Chester ◽  
Coralie Manavella ◽  
Yasmina Mallouk ◽  
...  
Keyword(s):  
Dermacentor Reticulatus ◽  
Babesia Canis ◽  
Babesia Canis Canis

The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

2013 ◽  
Vol 16 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Ł. Adaszek ◽  
M. Jankowska ◽  
M. Kalinowski ◽  
T. Banach ◽  
D. Wułupek ◽  
...  
Keyword(s):  
High Specificity ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Loop Mediated Isothermal Amplification ◽  
Specificity And Sensitivity ◽  
Amplification Assay ◽  
Analytical Laboratories ◽  
Babesia Canis Canis

Abstract The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.

Molecular detection and genetic diversity of norovirus genogroup IV: a yearlong monitoring of sewage throughout Italy

2010 ◽  
Vol 155 (4) ◽  
pp. 589-593 ◽  
Author(s):  
Giuseppina La Rosa ◽  
Marcello Iaconelli ◽  
Manoochehr Pourshaban ◽  
Marta Fratini ◽  
Michele Muscillo
Keyword(s):  
Genetic Diversity ◽  
Molecular Detection

Molecular evidence of natural infection with Babesia canis canis in Croatia

2010 ◽  
Vol 58 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Mirna Brkljačić ◽  
Vesna Matijatko ◽  
Ivana Kiš ◽  
Nada Kučer ◽  
Jadranka Foršek ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genetic Variant ◽  
Natural Infection ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Babesia Canis ◽  
Nucleotide Difference ◽  
Dark Urine ◽  
Blood Smears ◽  
Babesia Canis Canis

The aim of the present study was to detect and characterise the species and subspecies of Babesia spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of Babesia canis . The results showed the detection of one subspecies, namely Babesia canis canis using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 samples, 99.7% identity (one nucleotide difference) in 11 samples and 99.4% identity (two nucleotides difference) in 5 samples with B. canis canis . The results of this study confirm the presence of B. canis canis in infected dogs in Croatia and demonstrate a slightly new genetic variant of Babesia subspecies.

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