The prevention of transmission of Babesia canis canis by Dermacentor reticulatus ticks to dogs using a novel combination of fipronil, amitraz and (S)-methoprene

2011 ◽  
Vol 179 (4) ◽  
pp. 343-350 ◽  
Author(s):  
Frans Jongejan ◽  
Josephus J. Fourie ◽  
S. Theodore Chester ◽  
Coralie Manavella ◽  
Yasmina Mallouk ◽  
...  
Keyword(s):  
Dermacentor Reticulatus ◽  
Babesia Canis ◽  
Babesia Canis Canis

West-to-east differences of Babesia canis canis prevalence in Dermacentor reticulatus ticks in Slovakia

2011 ◽  
Vol 180 (3-4) ◽  
pp. 191-196 ◽  
Author(s):  
Michaela Kubelová ◽  
Emil Tkadlec ◽  
Marek Bednář ◽  
Eva Roubalová ◽  
Pavel Široký
Keyword(s):  
Dermacentor Reticulatus ◽  
Babesia Canis ◽  
Babesia Canis Canis

Molecular detection of Babesia canis in Dermacentor reticulatus ticks collected in Slovakia

Biologia ◽  
2006 ◽  
Vol 61 (2) ◽  
Author(s):  
Darja Duh ◽  
Mirko Slovák ◽  
Ana Saksida ◽  
Katja Strašek ◽  
Miroslav Petrovec ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Vector Competence ◽  
Dermacentor Reticulatus ◽  
Molecular Evidence ◽  
Babesia Canis ◽  
Aetiological Agent ◽  
Canine Babesiosis ◽  
Babesia Canis Canis

AbstractDermacentor reticulatus ticks are recognized as the most important vectors of Babesia canis, the aetiological agent of canine babesiosis occurring throughout Europe. Vector competence of D. reticulatus for B. canis is well described and experimentally determined; however, by using molecular analysis it was proven so by one recent study in Russia. Herein, the additional molecular evidence of B. canis infection in D. reticulatus ticks collected in Slovakia is provided. Using PCR followed by sequencing of distinctive amplicons we determined the presence of Babesia canis canis in one of 100 tested adult ticks. Two zoonotic pathogens, Francisella tularensis and Coxiella burnetii, were previously isolated from D. reticulatus ticks in Slovakia. In our samples, we detected only the presence of F. tularensis.

Molecular characterisation of Babesia canis canis and Babesia canis vogeli from naturally infected European dogs

2002 ◽  
Vol 106 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Simone M. Cacciò ◽  
Boris Antunovic ◽  
Annabella Moretti ◽  
Vittorio Mangili ◽  
Albert Marinculic ◽  
...  
Keyword(s):  
Babesia Canis ◽  
Molecular Characterisation ◽  
Babesia Canis Canis

The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs

2013 ◽  
Vol 16 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Ł. Adaszek ◽  
M. Jankowska ◽  
M. Kalinowski ◽  
T. Banach ◽  
D. Wułupek ◽  
...  
Keyword(s):  
High Specificity ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Babesia Canis ◽  
Canine Babesiosis ◽  
Loop Mediated Isothermal Amplification ◽  
Specificity And Sensitivity ◽  
Amplification Assay ◽  
Analytical Laboratories ◽  
Babesia Canis Canis

Abstract The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.

Molecular evidence of natural infection with Babesia canis canis in Croatia

2010 ◽  
Vol 58 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Mirna Brkljačić ◽  
Vesna Matijatko ◽  
Ivana Kiš ◽  
Nada Kučer ◽  
Jadranka Foršek ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genetic Variant ◽  
Natural Infection ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Babesia Canis ◽  
Nucleotide Difference ◽  
Dark Urine ◽  
Blood Smears ◽  
Babesia Canis Canis

The aim of the present study was to detect and characterise the species and subspecies of Babesia spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of Babesia canis . The results showed the detection of one subspecies, namely Babesia canis canis using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 samples, 99.7% identity (one nucleotide difference) in 11 samples and 99.4% identity (two nucleotides difference) in 5 samples with B. canis canis . The results of this study confirm the presence of B. canis canis in infected dogs in Croatia and demonstrate a slightly new genetic variant of Babesia subspecies.

scholarly journals The southernmost foci of Dermacentor reticulatus in Italy and associated Babesia canis infection in dogs

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Emanuela Olivieri ◽  
Sergio A. Zanzani ◽  
Maria S. Latrofa ◽  
Riccardo P. Lia ◽  
Filipe Dantas-Torres ◽  
...  
Keyword(s):  
Dermacentor Reticulatus ◽  
Babesia Canis

scholarly journals Over 20 years of research on canine babesiosis at the Faculty of Veterinary Medicine of WULS-SGGW in Warsaw

2017 ◽  
Vol 73 (10) ◽  
pp. 606-612
Author(s):  
Wojciech Zygner ◽  
Olga Gójska-Zygner ◽  
Paweł Górski ◽  
Justyna Bartosik
Keyword(s):  
Veterinary Medicine ◽  
Case Reports ◽  
Dermacentor Reticulatus ◽  
Endocrine Disorders ◽  
Babesia Canis ◽  
Pathological Changes ◽  
Canine Babesiosis ◽  
Euthyroid Sick Syndrome ◽  
Veterinary Surgeons ◽  
Pituitary Thyroid Axis

Canine babesiosis is a tick-borne disease caused by infection with protozoa of the genus Babesia. During over 20 years of studies on canine babesiosis at the Faculty of Veterinary Medicine of the Warsaw University of Life Sciences, the researchers have identified the species of the parasite and its vector in Poland, determined the cause of azotemia and observed other pathological changes, such as endocrine disorders, changes in the de Ritis quotient, as well as biochemical and hematological changes. It was shown that the tick Dermacentor reticulatus is the only vector of canine babesiosis in Poland and the disease is caused by Babesia canis (formerly known as Babesia canis canis). The first studies of pathological changes confirmed the results of previous research in other countries, in which the most prevalent changes in canine babesiosis were thrombocytopenia, anemia leucopenia, increased activity of liver enzymes (ALT, AST, ALP), azotemia, hypoalbuminemia, hypoglycemia, hyponatremia and hypokalemia. Research on azotemia in dogs infected with B. canis showed the contribution of TNF-α overproduction to hypotension and its influence on renal ischemia, hypoxia and the development of azotemia. Moreover, in research on endocrine disorders in canine babesiosis, it was shown that IL-6 overproduction leads to the suppression of the hypothalamic-pituitary-thyroid axis resulting in euthyroid sick syndrome, and that secondary hyperaldosteronism results from decreased renal blood flow and leads to hypokalemia. The results of the research were published in 27 original papers, 6 case reports and many review articles in veterinary journals. These studies provided veterinary surgeons in Poland with improved procedures for diagnosing canine babesiosis, as well as allowed them to better understand some pathogenic mechanisms of the disease and to develop more efficacious therapies..

scholarly journals Epizootiological Study of the Occurrence of Canine Babesiosisin Southwestern Slovakia

2016 ◽  
Vol 60 (3) ◽  
pp. 39-42
Author(s):  
K. Řeháčková ◽  
M. Haláková ◽  
B. Víchová ◽  
A. Kočišová
Keyword(s):  
Ixodes Ricinus ◽  
Clinical Signs ◽  
Dermacentor Reticulatus ◽  
Babesia Canis ◽  
Molecular Method ◽  
Canine Babesiosis ◽  
Blood Smears ◽  
Loss Of Appetite ◽  
Representative Samples

Abstract This epizootiological study was carried out to investigate the occurrence of canine babesiosis in southwestern Slovakia. The study focused on the proportion of the species of ticks serving as babesia vectors of babesiosis in the ticks collected from selected locations close to Komárno, in southwestern Slovakia. Additionally, observations were made on the health and overall clinical signs in dogs suspected of having babesiosis. In 2014 we collected ticks from vegetation by the drag cloth (flagging) method and also directly from dogs. A totally of 622 ticks were collected: 491 Dermacentor reticulatus and 131 Ix-odes ricinus. Representative samples of ticks (n = 103) were examined by the molecular method and the presence of Babesia spp. DNA was identified in 12.5 % of the Ixodes ricinus ticks collected by flagging and in 9.5 % of the Ixodes ricinus ticks collected from the dogs. Babesia canis (KU681325) with 90 bp sequence, 100 % identical with Babesia canis isolates from dogs for example from: Turkey (KF499115), Rumania (HQ662634), Croatia (FJ209025), Poland (EU622792) and Russia (AY962186), was confirmed after sequencing in one Ixodes ricinus female obtained from a dog. This was the first confirmation of the occurrence of B. canis in Ixodes ricinus ticks in Slovakia. In 2.2 % of the Dermacentor reticulatus ticks obtained from vegetation by flagging, we were able to diagnose the DNA of Babesia canis. In 4.8 % of the Dermacentor reticulatus ticks collected from dogs, the presence of Babesia spp. was confirmed. Thirty three dogs with suspicion of babesiosis were observed in an ambulance by their health and clinical signs. The loss of appetite was observed in 22 patients (66.7 %), apathy in 19 cases (57.6 %), and fever in 19 cases. Closer specification indicating babesiosis was finding engorged ticks on the dog bodies which occurred in 21 cases (63.6 %), haematuria in 8 cases (24.2 %), anaemia in 4 cases (12.1 %), and tremor in 6 cases (18.2 %). Other non-specific signs, such as diarrhoea, vomitus, slowed-down movement, ataxia and lacrimation were also observed in less than 18 % of the examined dogs. On the basis of the specific clinical signs, blood was withdrawn from 33 dogs for preparation of blood smears and in 19 of them (57.6 %) babesia in erythrocytes were confirmed microscopically.

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