Anemia associated with extraerythrocytic oxidative stress damage mediated by neutrophil superoxide anion production in chronic renal failure patients undergoing hemodialysis

Our goals were to determine whether acute exposure to nicotine alters neuronal nitric oxide synthase (nNOS)-dependent reactivity of cerebral arterioles and to identify a potential role for oxidative stress in nicotine-induced impairment in nNOS-dependent responses of cerebral arterioles. We measured in vivo diameter of cerebral arterioles to nNOS-dependent ( N-methyl-d-aspartate and kainate) and -independent (nitroglycerin) agonists before and during acute treatment with nicotine. We found that nNOS-dependent, but not -independent, vasodilatation was impaired during treatment with nicotine. In addition, treatment of the cerebral microcirculation with tempol (1 h before infusion of nicotine) prevented nicotine-induced impairment in nNOS-dependent vasodilatation. Furthermore, the production of superoxide anion (lucigenin chemiluminescence) was increased in parietal cortex tissue of rats by treatment with nicotine, and this increase in superoxide anion production could be inhibited by tempol. Our findings suggest that acute exposure to nicotine impairs nNOS-dependent dilatation of cerebral arterioles by a mechanism that appears to be related to the formation of superoxide anion.

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Eugenol prevents fMLF-induced superoxide anion production in human neutrophils by inhibiting ERK1/2 signaling pathway and p47phox phosphorylation

Scientific Reports ◽

10.1038/s41598-019-55043-8 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

Amina Chniguir ◽

Coralie Pintard ◽

Dan Liu ◽

Pham My-Chan Dang ◽

Jamel El-Benna ◽

...

Keyword(s):

Superoxide Anion ◽

Human Neutrophils ◽

Syzygium Aromaticum ◽

Superoxide Anion Production ◽

Anion Production ◽

Reduction Assay ◽

Cytochrome C Reduction ◽

Phenolic Group ◽

Anti Oxidant ◽

Neutrophil Superoxide

AbstractEugenol is a polyphenol extracted from Syzygium aromaticum essential oil. It is known to have anti-inflammatory and chemoprotective properties as well as a potent anti-oxidant activity due the presence of its phenolic group. In this study, we examined the effects of eugenol on neutrophil superoxide production, a key process involved in innate immunity and inflammation. Superoxide anion generationin human neutrophils was measured by cytochrome c reduction assay. Western blotting was used to analyze the phosphorylation of, p47phox, MAPKinases (p38 and ERK1/2), MEK1/2 and Raf, key proteins involved in the activation of NADPH oxidase. Pretreatment of neutrophils by increasing concentrations (2.5 µg/mL–20 µg/mL) of eugenol for 30 min, inhibited significantly (p < 0.001) superoxide anion generation induced by the chemotactic peptide formyl-Met-Leu-Phe (fMLF) with an IC50 of 5 µg/mL. Phorbolmyristate acetate (PMA)-stimulated O2− production was affected only at the highest eugenol concentration (20 µg/mL). Results showed that eugenol decreased the phosphorylation of p47phox onSer-345 and Ser-328, the translocation of p47phox to the membranesand the phosphorylation of Raf, MEK1/2 and ERK1/2 proteins. Taken together, our results suggest that eugenol inhibits the generation of superoxide anion by neutrophils via the inhibition of Raf/MEK/ERK1/2/p47phox-phosphorylation pathway.

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The Effect of Hypertonic and Colloid Resuscitation on Neutrophil Superoxide Anion Production and CD11b Expression After Burn

Journal of Burn Care & Rehabilitation ◽

10.1097/00004630-200103002-00123 ◽

2001 ◽

Vol 22 ◽

pp. S108

Author(s):

J. E. Stroh ◽

N. Fazal ◽

F. Haque ◽

R. L. Gamelli ◽

M. M. Sayeed

Keyword(s):

Superoxide Anion ◽

Superoxide Anion Production ◽

Cd11b Expression ◽

Anion Production ◽

Neutrophil Superoxide

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Plasma-Mediated Stimulation of Neutrophil Superoxide Anion Production During Coronary Artery Bypass Grafting: Role of Endothelin-1

The Thoracic and Cardiovascular Surgeon ◽

10.1055/s-2007-1013129 ◽

1999 ◽

Vol 47 (03) ◽

pp. 144-147 ◽

Cited By ~ 5

Author(s):

P. Bugajski ◽

R. Kalawski ◽

M. Baliński ◽

H. Wysocki ◽

R. Olszewski ◽

...

Keyword(s):

Coronary Artery ◽

Coronary Artery Bypass Grafting ◽

Superoxide Anion ◽

Artery Bypass ◽

Bypass Grafting ◽

Superoxide Anion Production ◽

Anion Production ◽

Neutrophil Superoxide ◽

Stimulation Of

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In vitro characterisation of fresh and frozen sex-sorted bull spermatozoa

Reproduction Fertility and Development ◽

10.1071/rd16086 ◽

2017 ◽

Vol 29 (7) ◽

pp. 1415 ◽

Cited By ~ 4

Author(s):

Shauna A. Holden ◽

Craig Murphy ◽

Juan F. Moreno ◽

Stephen T. Butler ◽

Andrew R. Cromie ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Anion ◽

Superoxide Anion Production ◽

Holstein Friesian ◽

Anion Production ◽

And Oxidative Stress

This study sought to compare the in vitro characteristics of fresh and frozen non-sorted (NS) and sex-sorted (SS) bull spermatozoa. Experiment 1: Holstein–Friesian ejaculates (n = 10 bulls) were split across four treatments and processed: (1) NS fresh at 3 × 106 spermatozoa, (2) X-SS frozen at 2 × 106 spermatozoa, (3) X-SS fresh at 2 × 106 spermatozoa and (4) X-SS fresh at 1 × 106 spermatozoa. NS frozen controls of 20 × 106 spermatozoa per straw were sourced from previously frozen ejaculates (n = 3 bulls). Experiment 2: Aberdeen Angus ejaculates (n = 4 bulls) were split across four treatments and processed as: (1) NS fresh 3 × 106 spermatozoa, (2) Y-SS fresh at 1 × 106 spermatozoa, (3) Y-SS fresh at 2 × 106 spermatozoa and (4) X-SS fresh at 2 × 106 spermatozoa. Controls were sourced as per Experiment 1. In vitro assessments for progressive linear motility, acrosomal status and oxidative stress were carried out on Days 1, 2 and 3 after sorting (Day 0 = day of sorting. In both experiments SS fresh treatments had higher levels of agglutination in comparison to the NS fresh (P < 0.001), NS frozen treatments had the greatest PLM (P < 0.05) and NS spermatozoa exhibited higher levels of superoxide anion production compared with SS spermatozoa (P < 0.05). Experiment 1 found both fresh and frozen SS treatments had higher levels of viable acrosome-intact spermatozoa compared with the NS frozen treatments (P < 0.01).

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Lipoxins control neutrophil superoxide anion production by regulation of polyisoprenyl diphosphate phosphatase 1 activity

The FASEB Journal ◽

10.1096/fasebj.27.1_supplement.137.2 ◽

2013 ◽

Vol 27 (S1) ◽

Author(s):

Troy Carlo ◽

Hermann Kalwa ◽

Bruce D Levy

Keyword(s):

Superoxide Anion ◽

Superoxide Anion Production ◽

Anion Production ◽

Neutrophil Superoxide

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Superoxide anion production and degranulation of rat neutrophils in response to acetaldehyde-altered liver cell membranes

Clinical Science ◽

10.1042/cs0710313 ◽

1986 ◽

Vol 71 (3) ◽

pp. 313-318 ◽

Cited By ~ 11

Author(s):

A. J. K. Williams ◽

R. E. Barry

Keyword(s):

Liver Cell ◽

Superoxide Anion ◽

Cell Membranes ◽

Membrane Vesicles ◽

Enzyme Release ◽

Superoxide Anion Production ◽

Liver Membrane ◽

Anion Production ◽

Neutrophil Superoxide ◽

Altered Liver

1. Rat liver membrane vesicles were exposed to acetaldehyde, with or without reduction of the resultant adducts formed. 2. Superoxide anion production and degranulation of rat neutrophils, upon stimulation with the liver membrane vesicles, were measured by cytochrome c reduction before and after the addition of superoxide dismutase, and β-glucuronidase release respectively. 3. Preincubation with acetaldehyde significantly enhanced superoxide anion production by both the reduced and non-reduced membrane samples (1.7-fold and 4.4-fold, respectively). 4. Preincubation with acetaldehyde significantly enhanced degranulation (1.5-fold) of neutrophils in response to the non-reduced membranes only. The reductive process itself caused a marked increase (2.4-fold) in the ability of the membrane vesicles to stimulate degranulation. 5. Cytochalasin B, an inhibitor of phagocytosis, did not reduce degranulation, implying that it occurred as a consequence of cell surface stimulation. 6. Neutrophil superoxide anion production and lysosomal enzyme release in response to acetaldehyde-altered liver cell membranes could be an important mechanism of hepatocyte injury in alcoholic liver disease.

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