Expression and purification of human respiratory syncytial virus recombinant fusion protein

2008 ◽  
Vol 62 (2) ◽  
pp. 146-152 ◽  
Author(s):  
Helen A. Arcuri ◽  
Luciano H. Apponi ◽  
Sandro R. Valentini ◽  
Edison L. Durigon ◽  
Walter F. de Azevedo ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Fusion Protein ◽  
Human Respiratory Syncytial Virus ◽  
Recombinant Fusion Protein ◽  
Expression And Purification ◽  
Virus Recombinant ◽  
Syncytial Virus

Molecular characterization of the glycoprotein and fusion protein in human respiratory syncytial virus subgroup A: Emergence of ON-1 genotype in Iran

2019 ◽  
Vol 71 ◽  
pp. 166-178 ◽  
Author(s):  
Somayeh Shatizadeh Malekshahi ◽  
Shaghayegh Razaghipour ◽  
Yazdan Samieipoor ◽  
Farhad B. Hashemi ◽  
Ali Akbar Rahbari Manesh ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Fusion Protein ◽  
Molecular Characterization ◽  
Human Respiratory Syncytial Virus ◽  
Syncytial Virus

scholarly journals Molecular Epidemiology and Phylodynamics of the Human Respiratory Syncytial Virus Fusion Protein in Northern Taiwan

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e64012 ◽  
Author(s):  
Hsin Chi ◽  
Hsin-Fu Liu ◽  
Li-Chuan Weng ◽  
Nai-Yu Wang ◽  
Nan-Chang Chiu ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Fusion Protein ◽  
Molecular Epidemiology ◽  
Human Respiratory Syncytial Virus ◽  
Virus Fusion ◽  
Syncytial Virus ◽  
Northern Taiwan

Immunovirological studies on human respiratory syncytial virus structural proteins

1986 ◽  
Vol 32 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Michel Trudel ◽  
Francine Nadon ◽  
Cécile Séguin ◽  
Simone Ghoubril ◽  
Pierre Payment ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Fusion Protein ◽  
Neutralizing Antibodies ◽  
Sodium Dodecyl ◽  
Human Respiratory Syncytial Virus ◽  
Structural Proteins ◽  
Membrane Glycoproteins ◽  
Protein Dimer ◽  
Syncytial Virus ◽  
Nucleoprotein N

Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), the nucleoprotein N (42 000), and a protein (78 000) designated P that could be the equivalent of the polymerase of the morbillivirus and paramyxovirus genus. Neutralizing monoclonal antibodies showed, by immunoprecipitation and immunoblotting, that the fusion protein carries neutralizing epitopes. One monoclonal antibody, which shows a high neutralizing titer, immunoblotted directly with the F1 fragment (49 000) of the fusion protein. Analysis in mice of the immunogenicity of the structural proteins separated on sodium dodecyl sulphate gels indicated that, under our conditions, only the fusion protein dimer Fo and its F1 fragment were capable of inducing neutralizing antibodies.

scholarly journals Thermostability of the human respiratory syncytial virus fusion protein before and after activation: implications for the membrane-fusion mechanism

2004 ◽  
Vol 85 (12) ◽  
pp. 3677-3687 ◽  
Author(s):  
M. Begoña Ruiz-Argüello ◽  
Diana Martín ◽  
Steve A. Wharton ◽  
Lesley J. Calder ◽  
Steve R. Martín ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Fusion Protein ◽  
Membrane Fusion ◽  
Human Respiratory Syncytial Virus ◽  
Virus Fusion ◽  
Before And After ◽  
Syncytial Virus

scholarly journals Effect of Proteolytic Processing at Two Distinct Sites on Shape and Aggregation of an Anchorless Fusion Protein of Human Respiratory Syncytial Virus and Fate of the Intervening Segment

Virology ◽  
2002 ◽  
Vol 298 (2) ◽  
pp. 317-326 ◽  
Author(s):  
M. Begoña Ruiz-Argüello ◽  
Luis González-Reyes ◽  
Leslie J. Calder ◽  
Concepción Palomo ◽  
Diana Martı́n ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Fusion Protein ◽  
Human Respiratory Syncytial Virus ◽  
Proteolytic Processing ◽  
Syncytial Virus

scholarly journals Electron Microscopy of the Human Respiratory Syncytial Virus Fusion Protein and Complexes That It Forms with Monoclonal Antibodies

Virology ◽  
2000 ◽  
Vol 271 (1) ◽  
pp. 122-131 ◽  
Author(s):  
Leslie J. Calder ◽  
Luis González-Reyes ◽  
Blanca García-Barreno ◽  
Steve A. Wharton ◽  
John J. Skehel ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibodies ◽  
Respiratory Syncytial Virus ◽  
Fusion Protein ◽  
Human Respiratory Syncytial Virus ◽  
Virus Fusion ◽  
Syncytial Virus
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